Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,140,040 A→C 11.6% I397L (ATT→CTT)  cusB → CzcB family copper RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,140,0400AC11.6% 91.7 / 6.2 41I397L (ATT→CTT) cusBCzcB family copper RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (21/15);  new base C (2/3);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

GACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGAT  >  NC_002947/6139896‑6140188
                                                                                                                                                |                                                                                                                                                    
gACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGAGCGACTCCGAAGCAAGCCTCAAGGGTAttgag                                                                                                                                                 >  1:640658‑M2/1‑145 (MQ=255)
gACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCAACGGGGCAATTCCTGATCGACTCCGAAGCAAGCCGCAAGGGTAttgag                                                                                                                                                 >  1:449744‑M2/1‑145 (MQ=255)
   cAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAAAGAAGTTTAAGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGCAAATCGTGGCATTCCGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGTTATTGAGGcc                                                                                                                                              >  1:1806805/1‑150 (MQ=255)
     aGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGccac                                                                                                                                            <  1:1280753/150‑1 (MQ=255)
      ggCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGccac                                                                                                                                            >  1:475217/1‑149 (MQ=255)
      ggCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGccac                                                                                                                                            <  2:475217/149‑1 (MQ=255)
              ccGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATc                                                                                                                                   <  1:1794022/150‑1 (MQ=255)
              atctccAATATAGGTCGTGCTAGCCCAGGAGAACGGCTGCCACTTCTCAATCTTCAAAGGCCTGCAGGCCGGGCAGAAACTCGTGGCATCCTTGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATc                                                                                                                                   <  1:927338/146‑1 (MQ=255)
                 cccAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGa                                                                                                                                >  1:302978/1‑150 (MQ=255)
                 cccAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGa                                                                                                                                <  1:1075130/150‑1 (MQ=255)
                 cccAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGa                                                                                                                                <  2:656930/150‑1 (MQ=255)
                   cAATAGAGGTGGTGCTAGGCAAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGGCTGCAGGCCGGGCAGAAAGTCGTGGCATCAGGGCAATTCCTGATCGACTCCGAAGAAAGACTCAAGGGTATTGAGGCCACAAGAGCAtagagat                                                                                                                              >  2:927338/1‑144 (MQ=255)
                     aTAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCa                                                                                                                            >  1:1470994/1‑150 (MQ=255)
                             ggtgCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCgcctgc                                                                                                                    <  1:1208491/150‑1 (MQ=255)
                                              aCGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTACTGAGGCTACCACAGCATCGGTTTCACCGCCTGCCTCGCAAGCACCTGAAc                                                                                                   <  2:221627/150‑1 (MQ=255)
                                                ggcggcCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAAccc                                                                                                 >  2:360023/1‑150 (MQ=255)
                                                          tCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcggagtcgatcaggaattgcccggatgccac                                                                                                          >  1:1553627‑M2/1‑87 (MQ=255)
                                                          tCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcggagtcgatcaggaattgcccggatgccac                                                                                                          <  2:1553627‑M2/131‑45 (MQ=255)
                                                            gCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCAc                                                                                                                           >  1:1202097/1‑112 (MQ=255)
                                                            gCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCAc                                                                                                                           <  2:1202097/112‑1 (MQ=255)
                                                                  ttGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcgga                                                                                                                                     <  1:1401226‑M2/96‑18 (MQ=255)
                                                                  ttGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcgga                                                                                                                                     >  2:1401226‑M2/1‑79 (MQ=255)
                                                                   tGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATg                                                                              <  1:916347/150‑1 (MQ=255)
                                                                           cTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTAttgag                                                                                                                                                 >  2:456940‑M2/1‑70 (MQ=255)
                                                                           cTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTAttgag                                                                                                                                                 <  1:456940‑M2/75‑6 (MQ=255)
                                                                                 gCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGAAAGACTGGCGGGGAGTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACaa                                                                >  2:1719593/1‑150 (MQ=255)
                                                                                         gAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAgg                                                        <  2:302978/150‑1 (MQ=255)
                                                                                                gTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAg                                                 >  1:162673/1‑150 (MQ=255)
                                                                                                      tCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCgtgt                                                                       >  1:350319/1‑122 (MQ=255)
                                                                                                      tCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCgtgt                                                                       <  2:350319/122‑1 (MQ=255)
                                                                                                       ccGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCa                                          <  1:1329770/150‑1 (MQ=255)
                                                                                                                   tGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGc                              >  2:1857449/1‑150 (MQ=255)
                                                                                                                   tGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGc                              >  1:90915/1‑150 (MQ=255)
                                                                                                                    gATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAACCGCAGGCACCCAGCTGACCATCGACCACGGGcc                             >  1:499946/1‑150 (MQ=255)
                                                                                                                        caCTCCGAAGAAAGCCTTAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGTACCCATCTTACCATCGACCACGGGCCATTc                         <  2:689036/149‑1 (MQ=255)
                                                                                                                           tCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTc                      >  2:1198425/1‑150 (MQ=255)
                                                                                                                             cGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCAc                    >  1:564805/1‑150 (MQ=255)
                                                                                                                             cGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCATGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCAc                    >  2:1927436/1‑150 (MQ=255)
                                                                                                                                        tCAAGGGTCTTTACGATACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGc         <  2:1806805/150‑1 (MQ=255)
                                                                                                                                        tCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGc         >  1:568619/1‑150 (MQ=255)
                                                                                                                                               tATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGATCATGCGCCATTCGTCACCTTGGGAATGCCTGGGat  >  2:1610122/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGCCTGGGAT  >  NC_002947/6139896‑6140188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑