Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	484,203	C→G	5.9%	G80A (GGC→GCC)	yeaG ←	protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	484,203	0	C	G	5.9%	237.2 / 2.9	77	G80A (GGC→GCC)	yeaG	protein kinase
Reads supporting (aligned to +/- strand):  ref base C (33/39);  new base G (3/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGATCC  >  NC_002947/484056‑484352                                                                                                                                                    |                                                                                                                                                      ttttCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTccatgcca                                                                                                                                                     <  1:201549/150‑1 (MQ=255) ttttCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTccatgcca                                                                                                                                                     <  1:1861771/150‑1 (MQ=255)    tCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATgg                                                                                                                                                  <  2:1419223/150‑1 (MQ=255)         cAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTc                                                                                                                                             <  2:259783/150‑1 (MQ=255)          aGCCGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCg                                                                                                                                            <  2:961694/150‑1 (MQ=255)           gctgctTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCgg                                                                                                                                           <  1:1271502/150‑1 (MQ=255)            ctgctTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGAGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGc                                                                                                                                          >  2:608084/1‑150 (MQ=255)                  gAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTGTTCCATGCCATGGAAGTCGGCAAAGGc                                                                                                                                    <  1:1103813/150‑1 (MQ=255)                  gAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGGCATGGAAGTCGGCAAAGGc                                                                                                                                    >  1:1823515/1‑150 (MQ=255)                  gAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGc                                                                                                                                    >  1:1215719/1‑150 (MQ=255)                   aggttttCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGcc                                                                                                                                   <  1:507607/147‑1 (MQ=255)                   aGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGcc                                                                                                                                   <  1:1003645/150‑1 (MQ=255)                   aGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGcc                                                                                                                                   >  1:502814/1‑150 (MQ=255)                   aGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGcc                                                                                                                                   <  2:1406114/150‑1 (MQ=255)                    gTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCg                                                                                                                                  >  2:239779/1‑150 (MQ=255)                    gTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCg                                                                                                                                  <  2:502814/150‑1 (MQ=255)                     tttttCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCgg                                                                                                                                 >  2:287595/1‑150 (MQ=255)                             cAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAgcggc                                                                                                                         >  2:1711073/1‑150 (MQ=255)                             cAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAgcg                                                                                                                           <  1:553405/148‑1 (MQ=255)                             cAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAgcg                                                                                                                           >  2:553405/1‑148 (MQ=255)                             cAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGAGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAgcggc                                                                                                                         <  1:608084/150‑1 (MQ=255)                                 cGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGAt                                                                                                                     >  1:197186/1‑150 (MQ=255)                                 cGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGAt                                                                                                                     <  2:734715/150‑1 (MQ=255)                                   aGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGCTATTTCTCCGTTGCTCCAGTGCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATaa                                                                                                                   <  2:1390069/150‑1 (MQ=255)                                    ggACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAAc                                                                                                                  <  1:756167/150‑1 (MQ=255)                                    ggACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCCTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGCCGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAAc                                                                                                                  <  1:839713/150‑1 (MQ=255)                                       cTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCtt                                                                                                               <  1:349363/150‑1 (MQ=255)                                        ttGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCttg                                                                                                              >  2:475370/1‑150 (MQ=255)                                         tGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCttgt                                                                                                             <  2:1316801/150‑1 (MQ=255)                                          gccgccgccTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCttgtt                                                                                                            <  2:197186/150‑1 (MQ=255)                                            cgccgccTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCCTGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTgg                                                                                                          >  1:174574/1‑150 (MQ=255)                                                gccTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGagaa                                                                                                      <  1:1299424/150‑1 (MQ=255)                                                 ccTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGagaag                                                                                                     >  2:137374/1‑150 (MQ=255)                                                  cTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGGCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGagaaga                                                                                                    <  2:1823515/150‑1 (MQ=255)                                                       gggCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATgcgc                                                                                               <  2:1414929/150‑1 (MQ=255)                                                              gcagATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGcc                                                                                        >  1:1032937/1‑150 (MQ=255)                                                                  aTAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCg                                                                                                                                  <  2:819614/104‑1 (MQ=255)                                                                  aTAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCg                                                                                                                                  >  1:819614/1‑104 (MQ=255)                                                                      agGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTg                                                                                <  1:810986/150‑1 (MQ=255)                                                                         tcttGTTTCTTTTCTTCCAGGCCTTGGGGGGCGTGGCGGAAGTACGACACGAGCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGATGGTa                                                                             <  2:1783105/147‑1 (MQ=255)                                                                         aTCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTa                                                                             >  1:1121820/1‑150 (MQ=255)                                                                           cTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTaga                                                                           >  2:27217/1‑150 (MQ=255)                                                                            tGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTagag                                                                          <  1:239779/150‑1 (MQ=255)                                                                             gTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTagaga                                                                         >  1:1783105/1‑149 (MQ=255)                                                                                 cttctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTagag                                                                          <  2:1524556/145‑1 (MQ=255)                                                                                 cttctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTagag                                                                          >  1:1524556/1‑145 (MQ=255)                                                                                  ttctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATCGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCg                                                                    >  2:1717446/1‑150 (MQ=255)                                                                                  ttctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCg                                                                    >  1:1170133/1‑150 (MQ=255)                                                                                        tcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGc                                                              >  1:482021/1‑150 (MQ=255)                                                                                          cAGGCCTTGGGCGGCGTGGCAGAAGTACGAACCGATGTGGTAGATGCACCCCGGCAGGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGctc                                                            <  2:51068/150‑1 (MQ=255)                                                                                           aGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGctct                                                           >  1:1374428/1‑150 (MQ=255)                                                                                             gCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTgg                                                         <  2:1032937/150‑1 (MQ=255)                                                                                              ccTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGc                                                        <  1:475370/150‑1 (MQ=255)                                                                                              ccTCGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgt                                                                        >  1:1788591/1‑134 (MQ=255)                                                                                              ccTCGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgt                                                                        <  2:1788591/134‑1 (MQ=255)                                                                                                        cgTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATgg                                              <  2:482021/150‑1 (MQ=255)                                                                                                        cgTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATgg                                              <  1:27217/150‑1 (MQ=255)                                                                                                        cgTGGCGCAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAAGCCGCAGATCTGCGGACCCTCTTGTTGGAGAAGATGCGCGACAGCCTTGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATgg                                              <  1:137374/150‑1 (MQ=255)                                                                                                         gTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGc                                             <  1:287595/150‑1 (MQ=255)                                                                                                                  gTACGACACGATCTGGGCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAg                                    >  1:670116/1‑150 (MQ=255)                                                                                                                   tACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGga                                                                                                         >  2:992003/1‑80 (MQ=255)                                                                                                                   tACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGga                                                                                                         <  1:992003/80‑1 (MQ=255)                                                                                                                    aCGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGGTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGcc                                  >  1:624008/1‑150 (MQ=255)                                                                                                                      gACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTa                                                                             >  1:1002886/1‑105 (MQ=255)                                                                                                                      gACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTa                                                                             <  2:1002886/105‑1 (MQ=255)                                                                                                                         acGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCCTAAGCCTGGCGTTGCTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCACCGGCCGTTCg                             <  2:174574/150‑1 (MQ=255)                                                                                                                           gATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGgc                           >  2:1621357/1‑150 (MQ=255)                                                                                                                               tGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgtgt                                                                      >  1:564951/1‑103 (MQ=255)                                                                                                                               tGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgtgt                                                                      <  2:564951/103‑1 (MQ=255)                                                                                                                                 gTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATg                                               <  1:411631/124‑1 (MQ=255)                                                                                                                                 gTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATg                                               >  2:411631/1‑124 (MQ=255)                                                                                                                                   cGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTAGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATg                   <  2:1121820/150‑1 (MQ=255)                                                                                                                                     aTGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCg                 >  2:1308342/1‑150 (MQ=255)                                                                                                                                       gCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTa               >  1:1444594/1‑150 (MQ=255)                                                                                                                                       gCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGCATCGCGGCGCATAATCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTa               >  1:1494167/1‑150 (MQ=255)                                                                                                                                        cACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAg              >  2:55207/1‑150 (MQ=255)                                                                                                                                         cgactTCCATGGCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAgg             >  1:48497/4‑150 (MQ=255)                                                                                                                                          gactTCCATGGCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGc                                   >  2:1128761/3‑127 (MQ=255)                                                                                                                                          gactTCCATGGCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGc                                   <  1:1128761/125‑1 (MQ=255)                                                                                                                                                catgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTgg                                                         <  2:250396/99‑1 (MQ=255)                                                                                                                                                catgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTgg                                                         >  1:250396/1‑99 (MQ=255)                                                                                                                                                catgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCg      >  2:1895357/1‑150 (MQ=255)                                                                                                                                                 atgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTgg                                                                               >  1:1785782/1‑76 (MQ=255)                                                                                                                                                 atgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTgg                                                                               <  2:1785782/76‑1 (MQ=255)                                                                                                                                                 atgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGa     <  2:624008/150‑1 (MQ=255)                                                                                                                                                   gccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGATc   <  2:1170133/150‑1 (MQ=255)                                                                                                                                                    ccatgGAAGTCGGCAACGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGATcc  >  1:1033172/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TTTTCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGATCC  >  NC_002947/484056‑484352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑