| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 550,552 | C→G | 16.9% | intergenic (+30/‑112) | tufB → / → rpsJ | elongation factor Tu‑B/30S ribosomal protein S10 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 550,552 | 0 | C | G | 16.9% | 145.4 / 23.4 | 60 | intergenic (+30/‑112) | tufB/rpsJ | elongation factor Tu‑B/30S ribosomal protein S10 |
| Reads supporting (aligned to +/- strand): ref base C (24/25); new base G (5/5); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AACATTCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAA > NC_002947/550403‑550695 | aacaTTCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTc > 1:1559439/1‑150 (MQ=255) ttCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCg < 1:1550729/150‑1 (MQ=255) cAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg < 1:1924224/150‑1 (MQ=255) gATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggggg > 1:77472/1‑150 (MQ=255) gACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAACCCCCCGCTCAGCGGGGGttt < 2:1176554/150‑1 (MQ=255) aCTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGtttt < 1:1669464/150‑1 (MQ=255) ttACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGttttttta < 2:1559439/150‑2 (MQ=255) ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg < 1:1576136/136‑1 (MQ=255) ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCggg > 2:1576136/1‑136 (MQ=255) ccTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGCTTTTTTTATTg > 2:911596/1‑150 (MQ=255) aTCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GACCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGtt < 2:422721/150‑1 (MQ=255) tCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGCAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGCCCGATTGAAAGCACCCCCGCTCAGCGGGGGTTTTTAGTTTTGGGTTg > 1:1341833/1‑150 (MQ=25) aGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGaca > 1:914595/1‑150 (MQ=255) aGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGaca < 2:77472/150‑1 (MQ=255) cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGGttttttcaatc < 1:1534667/137‑6 (MQ=255) cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTGAGCGGGGGttttttcaatc > 2:1534667/1‑132 (MQ=255) cATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTaa < 1:1177062/150‑1 (MQ=255) cAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTggg > 1:607831/1‑150 (MQ=255) aGACGGTCTGCGATTCGCTATTAGTGCAGGCGGTAGTAACGTCGGCGCCGGTGTTGT‑TGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGTGGGTTTTGTTTATTGGGTTGACACTAATTGGGGGCGTCt > 2:1477996/1‑150 (MQ=255) gACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGATTTTTTTATTGGGTTGACACTAATTGGGGGCGTCta > 1:1897937/1‑150 (MQ=255) tCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGG‑TTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAAt < 2:364316/150‑1 (MQ=255) tGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATc > 1:865821/1‑150 (MQ=255) gcgcTTCGCTAGCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCa > 2:812152/1‑150 (MQ=255) cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCAc < 2:1322897/150‑1 (MQ=255) cgcTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCAc < 2:865821/150‑1 (MQ=255) ccGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTa < 2:607831/150‑1 (MQ=255) gTGAAGGCGGCCCTACCGTGCGCTCCCGC‑TTGTCGGCGAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGc < 1:1879117/150‑1 (MQ=255) gAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCgg > 2:447029/1‑150 (MQ=255) cGGTCGTCCCGTCCGCGCCGTCTTTGT‑TTCCAGAAGCTTCGAGTAATCCCTCGCCCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTAGTGGGTTGACACTAATTGGGGGCGTCTATAGAATCTCGCCTCCTTTTAGCGGGCGTa < 1:911596/150‑1 (MQ=255) cGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACa > 2:1025202/1‑150 (MQ=255) cGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACa < 2:914595/150‑1 (MQ=255) gCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTAAGTTTTTGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGc < 1:1477996/150‑1 (MQ=255) gTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCt < 1:689451/150‑1 (MQ=255) ggCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGt > 1:1140298/1‑150 (MQ=255) gCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc > 1:1662265/1‑150 (MQ=255) gCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc > 1:162736/1‑150 (MQ=255) cAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGaa > 2:5238/1‑96 (MQ=255) cAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGaa < 1:5238/96‑1 (MQ=255) aaaTCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGaa < 2:162736/150‑1 (MQ=255) atcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCa > 1:1669922/1‑150 (MQ=255) tcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTgg < 1:1403001/125‑1 (MQ=255) tcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTgg > 2:1403001/1‑125 (MQ=255) tcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa < 1:1937967/150‑1 (MQ=255) tcGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCaa < 1:447029/150‑1 (MQ=255) aGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGc > 2:783170/1‑150 (MQ=255) aCTCGACCGATTGAAAAAACCACCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGAGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCa > 1:1551897/1‑150 (MQ=255) cGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATc > 1:150589/1‑150 (MQ=255) ttGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGt > 2:975874/1‑150 (MQ=255) tGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTa < 1:1025202/150‑1 (MQ=255) aaaaaaCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc > 1:201380/1‑117 (MQ=255) aaaaaaCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTc < 2:201380/117‑1 (MQ=255) aaaaCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGt > 2:357313/1‑89 (MQ=255) aaaaCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGt < 1:357313/89‑1 (MQ=255) aaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAg < 2:1140298/150‑1 (MQ=255) aaaaCCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAg < 1:783170/150‑1 (MQ=255) aCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAAc < 1:1658171/101‑1 (MQ=255) aCCCCCGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAAc > 2:1658171/1‑101 (MQ=255) cccGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGa < 1:1868758/106‑1 (MQ=255) cccGCTGAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGa > 2:1868758/1‑106 (MQ=255) cccGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGaa > 2:587597/1‑150 (MQ=255) cccGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGaa > 2:1757696/1‑150 (MQ=255) | AACATTCAGATGACTGTTACCCTGATCAAGACCATCGCAATGGAAGACGGTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTTGT‑GGCCAAAATCATCGAGTAATCACTCGACCGATTGAAAAAACCCCCGCTCAGCGGGGGTTTTTTTTATTGGGTTGACACTAATTGGGGGCGTCTATAGAATCACGCCTCCTTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAA > NC_002947/550403‑550695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |