Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 978,168 G→A 5.8% R51H (CGC→CAC)  iscR → DNA‑binding transcriptional regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947978,1680GA 5.8% 201.7 / 2.2 69R51H (CGC→CAC) iscRDNA‑binding transcriptional regulator IscR
Reads supporting (aligned to +/- strand):  ref base G (33/32);  new base A (2/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

TGACTACCAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCATGCCG  >  NC_002947/978024‑978317
                                                                                                                                                |                                                                                                                                                     
tgactACCAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTGCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCgcagca                                                                                                                                                  <  2:466571/150‑1 (MQ=255)
     aCCAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTg                                                                                                                                             <  2:871576/150‑1 (MQ=255)
      ccAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCt                                                                                                                                              >  1:1045222/1‑148 (MQ=255)
      ccAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCt                                                                                                                                              <  2:1045222/148‑1 (MQ=255)
       cAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGt                                                                                                                                           >  1:103292/1‑150 (MQ=255)
       cAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGt                                                                                                                                           >  2:1633931/1‑150 (MQ=255)
       cAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGt                                                                                                                                           <  1:1900655/150‑1 (MQ=255)
       cAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCAGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCACAGCAGCCTGGt                                                                                                                                           >  1:1690064/1‑150 (MQ=255)
            gccattACGCCGTGAACGCCATGCTCGACCTGGCGTTGCACCCGAAGCATGGGCCTGTGTCTTTGCCCGATATTTCCGAGCGCCAGGGCATGTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCa                                                                                                                                      <  1:1447744/145‑1 (MQ=255)
            gCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCa                                                                                                                                      <  2:528068/150‑1 (MQ=255)
            gCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCa                                                                                                                                      <  2:1429079/150‑1 (MQ=255)
              cGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAgt                                                                                                                                    <  1:929529/150‑1 (MQ=255)
                aTACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCACAGCAGCCTGGTTTCCAgtgt                                                                                                                                  <  2:1690064/150‑1 (MQ=255)
                     ccGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGcgcg                                                                                                                             <  2:1325862/150‑1 (MQ=255)
                         gACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTcc                                                                                                                         >  2:1234852/1‑150 (MQ=255)
                           ccGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCGCTCTTATCTGGAGCAGCGGTTCGCCAACCTGCGCCGCAGCAGCCTGGCTTCCAGTTTCCGCGGtgacg                                                                                                                       >  2:891734/1‑146 (MQ=255)
                              caaTGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCg                                                                                                                    >  2:867299/3‑150 (MQ=255)
                               cATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCgg                                                                                                                   >  1:1422368/1‑150 (MQ=255)
                                aTGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGt                                                                                                                  <  1:1102187/150‑1 (MQ=255)
                                aTGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGt                                                                                                                  <  1:1940956/150‑1 (MQ=255)
                                 tggtCGACCTGGTGTTGCACGCGAAGCATGGGCCGATCTCTGTGGCCGATATTTCCGAGCGCCAAGGCCTTTCCCTCTCATATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTg                                                                                                                 <  1:18584/147‑1 (MQ=255)
                                   cTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTGTCTTATCTGGAGCAGCTGTCCGTCCCCATGCGGGGCAACAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGc                                                                                                               >  1:1398800/1‑150 (MQ=255)
                                   cTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGc                                                                                                               >  2:1053557/1‑150 (MQ=255)
                                   cTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCACAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGc                                                                                                               >  1:1343225/1‑150 (MQ=255)
                                       aCCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTAcc                                                                                                           >  1:330018/1‑150 (MQ=255)
                                        ccTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCa                                                                                                          >  1:1703782/1‑150 (MQ=255)
                                         cTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGttt                                                                                                                                         <  1:583505/118‑1 (MQ=255)
                                         cTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGttt                                                                                                                                         >  2:583505/1‑118 (MQ=255)
                                         cTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAg                                                                                                         <  2:1415490/150‑1 (MQ=255)
                                            gCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTg                                                                                                      >  1:218854/1‑150 (MQ=255)
                                             cGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGt                                                                                                     <  1:928621/150‑1 (MQ=255)
                                                   aCGCGAAGCATGGGCCGGTGTCTTTGGGCGATATTTCCGAGCGCCAGGGCATTTCCCTATCTTATTTGGAGCAGCTGTTCGCGACGGTGCACCGGAAAAGCCTGGTTTCAAGAGCTCGCGGGCGAGGCGGTTGCTTCACGCTGTcacgtg                                                                                               >  2:550251/1‑148 (MQ=255)
                                                      cgAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCa                                                                                            <  1:372422/150‑1 (MQ=255)
                                                       gAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCAt                                                                                           >  1:379815/1‑150 (MQ=255)
                                                       gAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCAt                                                                                           <  2:103292/150‑1 (MQ=255)
                                                        aaGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATg                                                                                          <  2:330018/150‑1 (MQ=255)
                                                           cATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCACAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGaa                                                                                       <  2:1343225/150‑1 (MQ=255)
                                                                gCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCAt                                                                                  <  2:1586241/150‑1 (MQ=255)
                                                                    gtgtCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTg                                                                                                                 <  2:336231/115‑1 (MQ=255)
                                                                    gtgtCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTg                                                                                                                 >  1:336231/1‑115 (MQ=255)
                                                                     tgtCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAgg                                                                             <  1:867299/150‑1 (MQ=255)
                                                                             gCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAg                                                                     <  2:628670/150‑1 (MQ=255)
                                                                             gCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAg                                                                     >  2:1448874/1‑150 (MQ=255)
                                                                              cagctatCTCCGAGCGCCAGGGCATTTCCCGCTCTCATCTTGAGCAGCTGCCCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCCGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAgg                                                                    <  1:891734/146‑1 (MQ=255)
                                                                               cGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGt                                                                   >  1:1738409/1‑150 (MQ=255)
                                                                               cGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGt                                                                   >  2:1768367/1‑150 (MQ=255)
                                                                                               cAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAAc                                                   <  1:1448874/150‑1 (MQ=255)
                                                                                               cAGGGCATTTCCCTCTCTTATATGGAGCAGCTGTTCGCTAAGCTGCGCCGCAGAAGCATGGTTTCGAGTGTGCGCGGTCCAGACGGTGGCTACCAGCTGTCGCGGGGCATGGAAACTTTCCAGGTGGCCCCGGTTATCGACGCGGTaaaa                                                   >  2:274423/1‑149 (MQ=255)
                                                                                                aGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACg                                                  >  1:106596/1‑150 (MQ=255)
                                                                                                  ggCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGaa                                                >  2:1216568/1‑150 (MQ=255)
                                                                                                   gCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAAt                                               <  2:1201642/150‑1 (MQ=255)
                                                                                                     aTTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCgg                                                                                                <  1:1365482/99‑1 (MQ=255)
                                                                                                     aTTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCgg                                                                                                >  2:1365482/1‑99 (MQ=255)
                                                                                                     aTTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCg                                             <  1:1633931/150‑1 (MQ=255)
                                                                                                          ccTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGa                                        >  1:258006/1‑150 (MQ=255)
                                                                                                          ccTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGa                                        >  1:1061727/1‑150 (MQ=255)
                                                                                                               ctTATCTGGAGCCGCTGTTCGCCAAGCTGCGCCGCACCAGTATGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCa                                   <  2:1859204/150‑1 (MQ=255)
                                                                                                                ttATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCAc                                  >  2:192967/1‑150 (MQ=255)
                                                                                                                ttATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGACATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCAc                                  >  2:251032/1‑150 (MQ=255)
                                                                                                                         gcagcTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTAc                                                                                                            <  2:332553/67‑1 (MQ=255)
                                                                                                                         gcagcTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTAc                                                                                                            >  1:332553/1‑67 (MQ=255)
                                                                                                                         gcagcTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAg                                                                     <  2:1500404/106‑1 (MQ=255)
                                                                                                                         gcagcTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAg                                                                     >  1:1500404/1‑106 (MQ=255)
                                                                                                                           agcTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAgg                       <  2:1422368/150‑1 (MQ=255)
                                                                                                                           agcTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTCATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAgg                       >  2:245935/1‑150 (MQ=255)
                                                                                                                                   gCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATcc                                                                                <  1:1195046/85‑1 (MQ=255)
                                                                                                                                   gCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATcc                                                                                >  2:1195046/1‑85 (MQ=255)
                                                                                                                                   gCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGt               >  1:1004883/1‑150 (MQ=255)
                                                                                                                                         cTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGAcgcg                                                         >  1:1258766/1‑102 (MQ=255)
                                                                                                                                         cTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTCGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGAcgcg                                                         <  2:1258766/102‑1 (MQ=255)
                                                                                                                                            cgccgcAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCAt      <  2:1738409/150‑1 (MQ=255)
                                                                                                                                             gccgcAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCATg     <  2:1004883/150‑1 (MQ=255)
                                                                                                                                               cgcAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCATGcc   <  1:1768367/150‑1 (MQ=255)
                                                                                                                                               cgcAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTCATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCATGcc   <  1:245935/150‑1 (MQ=255)
                                                                                                                                                gcagcaGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCATGCCg  >  1:57741/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGACTACCAAAGGCCGATACGCCGTGACCGCCATGCTCGACCTGGCGTTGCACGCGAAGCATGGGCCGGTGTCTTTGGCCGATATTTCCGAGCGCCAGGGCATTTCCCTCTCTTATCTGGAGCAGCTGTTCGCCAAGCTGCGCCGCAGCAGCCTGGTTTCCAGTGTGCGCGGTCCAGGCGGTGGCTACCAGCTGTCGCGGGGCATGGAAACCATCCAGGTGGCCCAGGTTATCGACGCGGTCAACGAATCGGTCGACGCCACCCGCTGCCAGGGGCTCGGTGACTGCCATGCCG  >  NC_002947/978024‑978317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑