Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	88,649	T→A	10.1%	A223A (GCA→GCT)	betC ←	choline‑sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	88,649	0	T	A	10.1%	204.4 / 13.4	69	A223A (GCA→GCT)	betC	choline‑sulfatase
Reads supporting (aligned to +/- strand):  ref base T (29/33);  new base A (4/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GTCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGT  >  NC_002947/88503‑88797                                                                                                                                                   |                                                                                                                                                     gTCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGcc                                                                                                                                                   <  1:55725/150‑1 (MQ=255)  tCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCt                                                                                                                                                  <  1:1633040/150‑1 (MQ=255)  tCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCt                                                                                                                                                  >  2:1644714/1‑150 (MQ=255)    tGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAAGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTgg                                                                                                                                                <  2:1662418/150‑1 (MQ=255)     gcagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCCCAGACAGCGTCTTGTTCTAATGTTCTAAGATGTGCAGCATGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGc                                                                                                                                               <  1:1492994/150‑1 (MQ=255)     gcagcaATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGc                                                                                                                                               >  1:970656/1‑150 (MQ=255)             tGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCg                                                                                                                                       <  1:1158097/150‑1 (MQ=255)               gCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTGGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGc                                                                                                                                     >  1:1838034/1‑150 (MQ=255)               gCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGc                                                                                                                                     >  1:964676/1‑150 (MQ=255)                           aTCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGCACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAAGGCAGTTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                         >  2:707106/1‑150 (MQ=255)                            tCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                        <  2:292188/150‑1 (MQ=255)                                   aGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCca                                                                                                                 <  2:1274011/150‑1 (MQ=255)                                     cTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCcaca                                                                                                               <  1:431030/150‑1 (MQ=255)                                           gcgcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                        >  2:1728658/1‑135 (MQ=255)                                           gcgcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                        <  1:1728658/135‑1 (MQ=255)                                             gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGCCCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                       <  2:1612247/150‑1 (MQ=255)                                             gcCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCAGCACGGGGCATGGGGATATCCACACCTTCGTa                                                                                                       >  1:1311673/1‑150 (MQ=255)                                               cGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATg                                                                                                                           >  1:1417999/1‑128 (MQ=255)                                               cGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATg                                                                                                                           <  2:1417999/128‑1 (MQ=255)                                               cGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGc                                                                                                     <  2:1659949/150‑1 (MQ=255)                                               cGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGc                                                                                                     >  1:1524937/1‑150 (MQ=255)                                                gAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGAAATCCCGGTGAATGCGGGTCGGGCTTTGCAGGACTGGACGCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCg                                                                                                    >  1:1399676/1‑150 (MQ=255)                                                       gcgcGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                              <  2:1109099/149‑1 (MQ=255)                                                       gcgcGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCa                                                                                              >  1:1109099/1‑149 (MQ=255)                                                                gCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                              >  2:115951/1‑92 (MQ=255)                                                                gCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCt                                                                                                                                              <  1:115951/92‑1 (MQ=255)                                                                gCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                        <  2:690569/114‑1 (MQ=255)                                                                gCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgggg                                                                                                                        >  1:690569/1‑114 (MQ=255)                                                                  aTCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGc                                                                                  <  2:1819796/150‑1 (MQ=255)                                                                     gcgAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTCgac                                                                                                                                     <  2:1043817/96‑3 (MQ=39)                                                                     gcgAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCAGCCAGGCAGAACTCgac                                                                                                                                     >  1:1043817/1‑94 (MQ=39)                                                                        aaCCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGgt                                                                            >  1:1619953/1‑150 (MQ=255)                                                                        aaCCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTCGCAAGGgt                                                                            <  2:964676/150‑1 (MQ=255)                                                                          ccTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGgtgt                                                                          >  2:1520762/1‑150 (MQ=255)                                                                           cTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACggg                                                                                                                                >  2:1220237/1‑95 (MQ=255)                                                                           cTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACggg                                                                                                                                <  1:1220237/95‑1 (MQ=255)                                                                            ttATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGat                                                                                                                      <  2:1395272/104‑1 (MQ=255)                                                                            ttATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGat                                                                                                                      >  1:1395272/1‑104 (MQ=255)                                                                            ttATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAg                                                                        >  2:1174398/1‑150 (MQ=255)                                                                              aTCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGGGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAggg                                                                      <  1:707106/150‑1 (MQ=255)                                                                                 cacaGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                         <  2:1347091/96‑1 (MQ=255)                                                                                 cacaGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATggg                                                                                                                         >  1:1347091/1‑96 (MQ=255)                                                                                  acaGGCAGTGGCTTGTTCCAAAGGTCGTGGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCa                                                                  <  2:1838034/150‑1 (MQ=255)                                                                                  acaGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCTTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCa                                                                  >  2:57412/1‑150 (MQ=255)                                                                                    aGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAg                                                                                                      >  1:1159850/1‑112 (MQ=255)                                                                                    aGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAg                                                                                                      <  2:1159850/112‑1 (MQ=255)                                                                                      gCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCg                                                              >  2:743083/1‑150 (MQ=255)                                                                                           ggCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                         <  2:1619953/150‑1 (MQ=255)                                                                                            gCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgg                                                                                                                          <  2:1822685/84‑1 (MQ=255)                                                                                            gCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATgg                                                                                                                          >  1:1822685/1‑84 (MQ=255)                                                                                                     aaaGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGaaa                                               <  2:1524937/150‑1 (MQ=255)                                                                                                            gTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAg                                        <  1:1644714/150‑1 (MQ=255)                                                                                                            gTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAg                                        <  2:662228/150‑1 (MQ=255)                                                                                                              aGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGc                                      >  2:1936016/1‑150 (MQ=255)                                                                                                                  cTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCaaaa                                  >  1:180406/1‑150 (MQ=255)                                                                                                                       gcagGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGTCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATggcc                             <  2:1399676/150‑1 (MQ=255)                                                                                                                          agcagtgtgAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGAGATGCGGGTGGGTCATTGAAACGCTCAGGCAAAATggccggc                          >  1:1037064/6‑150 (MQ=255)                                                                                                                               tgtgAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCg                     >  1:1278702/1‑150 (MQ=255)                                                                                                                                 tgAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACCCCTTCGTCGCGGTCTCTTTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGtt                   <  1:723285/150‑1 (MQ=255)                                                                                                                                    aTGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTTGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTc                <  1:547441/150‑1 (MQ=255)                                                                                                                                       cGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                         >  1:743618/1‑106 (MQ=255)                                                                                                                                       cGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCgggtgg                                                         <  2:743618/106‑1 (MQ=255)                                                                                                                                            cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCggg                                                            <  2:1408882/98‑1 (MQ=255)                                                                                                                                            cGAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCggg                                                            >  1:1408882/1‑98 (MQ=255)                                                                                                                                            cGAGTTCAGCCAGGCAGAACTCGACACGGGGCATGGGGATATc                                                                                                                   >  2:864440/1‑43 (MQ=14)                                                                                                                                            cGAGTTCAGCCAGGCAGAACTCGACACGGGGCATGGGGATATc                                                                                                                   <  1:864440/43‑1 (MQ=2)                                                                                                                                             gAGTTCAGCCAGGCAGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATg       >  2:1888497/1‑150 (MQ=255)                                                                                                                                                ttCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTc    <  2:1393867/150‑1 (MQ=255)                                                                                                                                                  cTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTTTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGt  >  2:1200617/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTCTGCAGCAATTGGCCGATGTTGTCATCGATATAGCTGCACGCGCCGAAGTAGGCGCGGCGGGCATCGCGAACCTTATCCACAGGCAGTGGCTTGTTCCAAAGGTCGTAGACCTTCAGCAGGCGCTGTGAATGCGGGTCGAGTTCTGCCTGGCTGAACTCGGCACGGGGCATGGGGATATCCACACCTTCGTAGCGGTCCCAGTAGCGTTTGGCAATGGTGTAGGGGTCATGCGGGTGGGTCATCGAAACGGTCAGGCAAAATGGCCGGCCATCGTTTTCGCGCACATGGTCGT  >  NC_002947/88503‑88797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑