Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,256,890 A→T 7.6% noncoding (431/451 nt) PP_mr14 ← cspA regulatory region

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,256,8900AT 7.6% 191.7 / 3.8 63noncoding (431/451 nt)PP_mr14cspA regulatory region
Reads supporting (aligned to +/- strand):  ref base A (31/27);  new base T (2/3);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAT  >  NC_002947/1256741‑1257031
                                                                                                                                                     |                                                                                                                                             
tACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGaa                                                                                                                                               <  2:930849/150‑1 (MQ=255)
   cATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAACAAACCCGCCAGAAGGc                                                                                                                                            >  1:271537/1‑150 (MQ=255)
            aGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGGGCGCGAGCCTGCGATGTGCCAATCTGCGATTGCTGGTAGGCATAAAAAAACCCGCCTTCTGGCGGGtttttt                                                                                                                                   <  1:1007213/150‑1 (MQ=255)
            aGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGtttttt                                                                                                                                   <  2:1832888/150‑1 (MQ=38)
                tGACCTGCCATACCGAGTTTGCCCGCACACGGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGATTTTTTGaa                                                                                                                               >  2:379338/1‑150 (MQ=255)
                   ccTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGc                                                                                                                            >  2:458315/1‑150 (MQ=255)
                     tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                          >  2:259364/1‑150 (MQ=255)
                     tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                          >  1:176304/1‑150 (MQ=255)
                     tGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                          <  1:1503763/150‑1 (MQ=255)
                       ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttttttt                                                                                                                                  <  2:1375635/140‑1 (MQ=38)
                       ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCTTCTGGCGGGttttttt                                                                                                                                  >  1:1375635/1‑140 (MQ=255)
                       ccATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAgg                                                                                                                        <  1:458315/150‑1 (MQ=255)
                          tACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAg                                                                                                                     <  1:1482732/150‑1 (MQ=255)
                             cGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTa                                                                                                                  >  1:1054492/1‑150 (MQ=255)
                             cGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGACCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTa                                                                                                                  <  2:1613706/150‑1 (MQ=255)
                                   tGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGa                                                                                                            <  1:259364/150‑1 (MQ=255)
                                      ccGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACtt                                                                                                         >  2:247815/1‑150 (MQ=255)
                                            acaGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGa                                                                                                   >  2:1747095/1‑150 (MQ=255)
                                                       gcCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCTGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCACAAAAAAACCCGCCTTCTGGCGGGttttt                                                                                                                                    >  1:1910665/1‑106 (MQ=25)
                                                        ccGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAActtctt                                                                                       <  1:1428485/150‑1 (MQ=255)
                                                         cGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttt                                                                                                                                      <  1:168359/102‑1 (MQ=255)
                                                         cGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGttt                                                                                                                                      >  2:168359/1‑102 (MQ=255)
                                                         cGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                      >  2:1815446/1‑150 (MQ=255)
                                                         cGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACttcttc                                                                                      >  2:1812982/1‑150 (MQ=255)
                                                            aCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGAt                                                                                                               <  2:533778/122‑1 (MQ=255)
                                                            aCTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGAt                                                                                                               >  1:533778/1‑122 (MQ=255)
                                                             cTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGcc                                                                                  <  1:247815/150‑1 (MQ=255)
                                                                gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                          <  2:574090/107‑1 (MQ=255)
                                                                gAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTa                                                                                                                          >  1:574090/1‑107 (MQ=255)
                                                                  aGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCAt                                                                             <  2:1582443/150‑1 (MQ=255)
                                                                     cTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGcc                                                                          >  1:563117/1‑150 (MQ=255)
                                                                          cAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGTAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTCGCAGACTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCt                                                                     <  1:1505834/150‑1 (MQ=39)
                                                                           aGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTg                                                                    <  1:1747095/150‑1 (MQ=255)
                                                                             tCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGc                                                                  >  1:1687802/1‑150 (MQ=255)
                                                                               ccTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcg                                                                >  2:1051362/1‑150 (MQ=255)
                                                                                 tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                       <  1:32113/109‑1 (MQ=255)
                                                                                 tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAg                                                                                                       >  2:32113/1‑109 (MQ=255)
                                                                                 tgtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgcg                                                              <  2:1054492/150‑1 (MQ=255)
                                                                                  gtgGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTTCATGCCTTTCTGGCCGCgg                                                             >  1:591631/1‑150 (MQ=255)
                                                                                    gggAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGt                                                           >  1:1767679/1‑150 (MQ=255)
                                                                                                 cccGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGaa                                                                                                                                               <  1:1017877/53‑1 (MQ=255)
                                                                                                 cccGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGaa                                                                                                                                               >  2:1017877/1‑53 (MQ=255)
                                                                                                  ccGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTaa                                             >  1:1737844/1‑150 (MQ=255)
                                                                                                   cgcgAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAAc                                            <  1:1467815/150‑1 (MQ=255)
                                                                                                         gagGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCt                                                                         >  2:1299540/1‑115 (MQ=255)
                                                                                                         gagGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCt                                                                         <  1:1299540/115‑1 (MQ=255)
                                                                                                            gCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGcc                                   >  1:850191/1‑150 (MQ=255)
                                                                                                             ccAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCct                                  >  2:275100/1‑150 (MQ=255)
                                                                                                               aGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCcttc                                >  1:398501/1‑150 (MQ=255)
                                                                                                                gcgcCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCTGCGGGTAGCGACGAAAGTAACAGCCTGGCcttct                               <  2:176304/150‑1 (MQ=255)
                                                                                                                   ccTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTc                            >  2:1072080/1‑150 (MQ=255)
                                                                                                                     tATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAg                          >  2:785905/1‑150 (MQ=255)
                                                                                                                           gAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                    >  2:165983/1‑150 (MQ=255)
                                                                                                                           gAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGtttt                    <  1:1812982/150‑1 (MQ=255)
                                                                                                                              ctggCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGaa                 <  1:1368427/148‑1 (MQ=255)
                                                                                                                              cAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAAc                                            >  1:346593/1‑123 (MQ=255)
                                                                                                                              cAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAAc                                            <  2:346593/123‑1 (MQ=255)
                                                                                                                                ggCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGc               <  1:1072080/150‑1 (MQ=255)
                                                                                                                                 gCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGcc              <  1:74580/150‑1 (MQ=255)
                                                                                                                                      aaaaaaCCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAg         >  2:1835438/1‑150 (MQ=255)
                                                                                                                                            cccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGa   <  2:386851/150‑1 (MQ=255)
                                                                                                                                             ccGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAt  <  2:1687802/150‑1 (MQ=255)
                                                                                                                                               gCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgc                                                               <  2:1536614/87‑1 (MQ=255)
                                                                                                                                               gCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCcgc                                                               >  1:1536614/1‑87 (MQ=255)
                                                                                                                                               gCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGttt                     <  1:839594/129‑1 (MQ=255)
                                                                                                                                               gCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGttt                     >  2:839594/1‑129 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
TACCATCAGCGAAGACTGACCTGCCATACCGAGTTTGCCCGCACACAGCGACTGCGCCGTACTTGAAGCCTGTGCAGTCCCTGTGGGAGCGGGCGAGCCCGCGAAGAGGCCAGCGCCTATTTCGAGCAGGCATAAAAAAACCCGCCAGAAGGCGGGTTTTTTTGAAAGCTAGGAAGCTAGATCGACTTAGGCGATCTGAACTTCTTCAGCCTGCATGCCTTTCTGGCCGCGGGTAGCGACGAAAGTAACAGCCTGGCCTTCTTTCAGGGTTTTGAAGCCGTCAGCTTGGAT  >  NC_002947/1256741‑1257031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑