| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,296,606 | G→A | 30.7% | intergenic (‑353/‑213) | nhaA‑I ← / → PP_1133 | Na(+)/H(+) antiporter NhaA/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,296,606 | 0 | G | A | 30.7% | 25.7 / 15.7 | 26 | intergenic (‑353/‑213) | nhaA‑I/PP_1133 | Na(+)/H(+) antiporter NhaA/transposase |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base A (4/4); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTACAGGGGACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTCGTGTGCGCGGGACAGAGATCGTAGCCAGCACTGGTGTGTGTCTGCACACCCGCGTAGGAGAGTGATCCGCACGGTATCGGCGTGAAGTCCCT > NC_002947/1296462‑1296722 | cTACAGGGGACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgccgcc > 2:233702/1‑150 (MQ=255) aCAGGGGACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCtt > 2:694151/1‑150 (MQ=255) ggggACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTc < 1:916704‑M1/150‑2 (MQ=255) gggACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCCCGGTGACATGTCTGCCAGCAAACGTGAGGGGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGc > 1:528968/1‑150 (MQ=14) gACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTAACGCGTCTGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCtt > 1:435546/1‑150 (MQ=255) aCCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCttt < 1:187695/150‑1 (MQ=39) cATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTCCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGt < 2:146777/150‑1 (MQ=38) aTTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGtt > 1:1166309/1‑150 (MQ=37) gTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAgg < 2:53692/150‑1 (MQ=34) ttCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgcc > 1:1637128/1‑131 (MQ=33) ttCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgcc < 2:1637128/131‑1 (MQ=33) ccGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGtt < 1:1220477/150‑1 (MQ=32) cGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGACAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTc < 2:72148/150‑1 (MQ=14) tgtAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTcttt < 1:1654504‑M1/137‑5 (MQ=255) tgtAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTcttt > 2:1654504‑M1/1‑133 (MQ=255) gCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCTACCGGGCCAACCGCCTTTTctt > 1:1335812‑M1/1‑117 (MQ=255) cGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCTACCGGGCCAACCGCCTTTTctt > 1:1464753‑M1/1‑75 (MQ=255) cGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCTACCGGGCCAACCGCCTTTTctt < 2:1464753‑M1/78‑4 (MQ=255) gAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacagtggtcgcgt > 1:1210988‑M1/1‑43 (MQ=255) gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaa < 2:587805‑M1/108‑78 (MQ=255) gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaa > 1:587805‑M1/1‑31 (MQ=255) ggTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcgaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgc < 2:1452819‑M1/84‑56 (MQ=255) ggTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcgaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgc > 1:1452819‑M1/1‑29 (MQ=255) ggTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacgggcga < 2:1679281‑M1/131‑103 (MQ=255) ggTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacgggcga > 1:1679281‑M1/1‑29 (MQ=255) ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacac > 2:812894‑M1/1‑29 (MQ=255) ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacac < 1:812894‑M1/127‑99 (MQ=255) gTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagg > 2:150851‑M1/1‑28 (MQ=255) gTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagg < 1:150851‑M1/107‑80 (MQ=255) | CTACAGGGGACCATTGTTCCGTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTCGTGTGCGCGGGACAGAGATCGTAGCCAGCACTGGTGTGTGTCTGCACACCCGCGTAGGAGAGTGATCCGCACGGTATCGGCGTGAAGTCCCT > NC_002947/1296462‑1296722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |