Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,330,507 G→A 7.4% intergenic (+32/‑275) PP_5SE → / → PP_1157 5S ribosomal RNA/acetolactate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,330,5070GA 7.4% 150.0 / 2.8 54intergenic (+32/‑275)PP_5SE/PP_11575S ribosomal RNA/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base G (28/22);  new base A (2/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

GATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTG  >  NC_002947/1330388‑1330652
                                                                                                                       |                                                                                                                                                 
gATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAAtt                                                                                                                     <  1:1010714/150‑1 (MQ=31)
 atcccatcccGAACTCAGTAGTGAAACGATGCATCGCTGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTCGGTGATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTg                                                                                                                    >  1:919578/1‑150 (MQ=12)
 atcccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTg                                                                                                                    <  1:1899033/150‑1 (MQ=32)
   cccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAAtt                                                                                                                     <  1:1325321/147‑1 (MQ=31)
   cccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAAtt                                                                                                                     >  2:1325321/1‑147 (MQ=31)
     catcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGAcc                                                                                                                >  2:768477/1‑150 (MQ=35)
       tcccGAACTCAGTAGTGAAACGATGGATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTg                                                                                                              >  1:897263/1‑150 (MQ=21)
        cccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGc                                                                                                             >  2:90131/1‑150 (MQ=35)
          cGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTg                                                                                                                    >  1:1123407/1‑141 (MQ=32)
          cGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTg                                                                                                                    <  2:1123407/141‑1 (MQ=32)
           gAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCg                                                                                                          >  2:539412/1‑150 (MQ=38)
                        aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCTTGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATgg                                                                                             <  1:101511/150‑1 (MQ=255)
                        aaaCGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATgg                                                                                             >  2:101511/1‑150 (MQ=255)
                               gCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGc                                                                                      <  1:90131/150‑1 (MQ=255)
                                       gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCgg                                                                              <  1:460708/150‑1 (MQ=255)
                                                gtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCgg                                                                     <  2:1309678/150‑1 (MQ=255)
                                                          cccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGc                                                           <  1:545017/150‑1 (MQ=255)
                                                           ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCt                                                          >  1:1860587/1‑150 (MQ=255)
                                                            cATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTc                                                         >  2:687794/1‑150 (MQ=255)
                                                              tgtgAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCt                                                       >  2:1808537/1‑150 (MQ=255)
                                                               gtgAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCTGGTAAACCCGCTCCTa                                                      >  1:554258/1‑150 (MQ=255)
                                                                 gagaGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACa                                                    >  2:231809/1‑150 (MQ=255)
                                                                               gTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTTTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGaa                                      >  2:195977/1‑150 (MQ=255)
                                                                                   aGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAAcc                                                              >  1:420387/1‑122 (MQ=255)
                                                                                   aGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAAcc                                                              <  2:420387/122‑1 (MQ=255)
                                                                                   aGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCa                                  <  2:897263/150‑1 (MQ=255)
                                                                                    gATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAg                                 <  1:1229547/150‑1 (MQ=255)
                                                                                     aTTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAAtt                                                                                                                     <  2:521483/65‑1 (MQ=33)
                                                                                     aTTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAAtt                                                                                                                     >  1:521483/1‑65 (MQ=33)
                                                                                     aTTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCgg                                                                     >  2:373068/1‑113 (MQ=255)
                                                                                     aTTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCgg                                                                     <  1:373068/113‑1 (MQ=255)
                                                                                     aTTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAgg                                <  1:768477/150‑1 (MQ=255)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTgg                                                                                                                   >  1:1552585/1‑62 (MQ=37)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTgg                                                                                                                   <  2:1552585/62‑1 (MQ=37)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCgaga                                                                                                       >  2:956559/1‑74 (MQ=255)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCgaga                                                                                                       <  1:956559/74‑1 (MQ=255)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCgg                                                                                       <  1:1353492/90‑1 (MQ=255)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCgg                                                                                       >  2:1353492/1‑90 (MQ=255)
                                                                                          tttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAAGTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGa                           >  1:523724/1‑150 (MQ=255)
                                                                                           ttCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGaa                          <  2:919578/150‑1 (MQ=255)
                                                                                             cGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTCCAGAGGATGGTGCGAAGCCAGGCTAGAATg                        <  1:1808537/150‑1 (MQ=255)
                                                                                             cGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATg                        <  1:231809/150‑1 (MQ=255)
                                                                                              gCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATgg                       >  2:858044/1‑150 (MQ=255)
                                                                                                 aaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTgg                                                                                                                   <  2:1722147/55‑1 (MQ=38)
                                                                                                 aaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTgg                                                                                                                   >  1:1722147/1‑55 (MQ=38)
                                                                                                     cccTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAAc                >  2:1658970/1‑150 (MQ=255)
                                                                                                     cccTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATAGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAAc                >  1:516604/1‑150 (MQ=255)
                                                                                                     cccTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGgtg                                                                            <  2:195707/90‑1 (MQ=37)
                                                                                                     cccTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGgtg                                                                            >  1:195707/1‑90 (MQ=37)
                                                                                                      ccTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACg               >  1:830580/1‑150 (MQ=255)
                                                                                                      ccTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGAGTTCGCGGGTAAACACGCTCGAAAAGAAGATGGTACGAAGCCAGGCTAGAATGGCCTCAAcc               >  2:1475169/1‑149 (MQ=37)
                                                                                                      ccTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACg               >  2:233658/1‑150 (MQ=38)
                                                                                                                 acgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGATATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACg    >  2:1256581/1‑150 (MQ=255)
                                                                                                                   gcaGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTg  <  1:233658/150‑1 (MQ=255)
                                                                                                                       |                                                                                                                                                 
GATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCGGGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTG  >  NC_002947/1330388‑1330652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑