| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,504,077 | T→A | 13.5% | intergenic (+35/‑228) | rpsI → / → petA | 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,504,077 | 0 | T | A | 13.5% | 138.9 / 13.9 | 52 | intergenic (+35/‑228) | rpsI/petA | 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/23); new base A (3/4); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
CTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGACG > NC_002947/1503929‑1504225 | cTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTg < 1:200152/150‑1 (MQ=255) tGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTgg > 1:27534/1‑150 (MQ=255) ggCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCg > 2:1172293/1‑150 (MQ=255) cgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCggg < 2:842685/150‑1 (MQ=255) cgtcgtGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCGGGCtttttt < 1:1043934/150‑1 (MQ=255) tGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGAGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAg > 1:237287/1‑150 (MQ=255) cTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGcc < 2:455476/150‑1 (MQ=255) ggCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCtt < 1:1762705/150‑1 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTg < 2:492995/150‑1 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGGCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTg > 1:452295/1‑150 (MQ=255) tACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGaa < 1:1412012/150‑1 (MQ=255) ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTg > 1:274156/1‑150 (MQ=255) ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTg > 2:1740989/1‑150 (MQ=255) gccgcgcgGGAAGTTGAGCTAAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGCCCTCATTACTCCAAGCGTTAATACCGCTTCGGCAGCCGAAAAAAGCCCGGTTCCTTGGACCCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcag < 2:105346/148‑1 (MQ=255) cGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcagca < 1:1117814/150‑1 (MQ=255) gAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTa > 1:762748/1‑121 (MQ=255) gAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTa < 2:762748/121‑1 (MQ=255) gTAAGAATGTGGGTATGAGTAAAGCGCGTAAGCGCCCTCATTACTCCAAGCGTTAATACCGCTGCGGCAGTCGAAAAAAGCCCGGTTCCTTTGTATCGCGCTTTTTGTTTAGCCTTCAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTc < 2:271313/150‑1 (MQ=255) aGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCa > 1:677363/1‑150 (MQ=255) aGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGaa > 2:517888/1‑112 (MQ=255) aGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGaa < 1:517888/112‑1 (MQ=255) gCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCa > 1:1172004/1‑150 (MQ=255) gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAg < 2:237287/150‑1 (MQ=255) attatgTATGCTTCCTCAGTCCTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTACCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCaaa < 2:700217/145‑1 (MQ=255) cgcgTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGc < 2:1172004/150‑1 (MQ=255) tAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg < 1:1172293/150‑1 (MQ=255) aaGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTggg < 2:27534/150‑1 (MQ=255) aGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAAGCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGt > 1:257493/1‑150 (MQ=255) cTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCgg > 2:1486269/1‑63 (MQ=255) cTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCgg < 1:1486269/63‑1 (MQ=255) cTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTg > 2:390377/1‑146 (MQ=255) cTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTg < 1:390377/146‑1 (MQ=255) aGTACTCCAAGAGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCt > 1:805742/1‑150 (MQ=255) gTACTCCAGGCTTTAATCCCGTTTCGGCAGTCGAAAAAGGCCCGGTTCCTTGGAACGGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCtt < 2:3979/150‑1 (MQ=255) tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg > 2:1563966/1‑150 (MQ=255) aaGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcagt < 1:152853/92‑1 (MQ=255) aaGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcagt > 2:152853/1‑92 (MQ=255) aaGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGc < 1:177039/95‑1 (MQ=255) aaGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGc > 2:177039/1‑95 (MQ=255) gCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTa > 1:1561870/1‑150 (MQ=255) gTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGAGCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGAGTaaa > 1:638850/1‑149 (MQ=255) cGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTAGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGAt > 1:1846743/1‑150 (MQ=255) aaaGCCCGGTTCCTTGGACCCGGGCTTTTTTTTTACCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATAAACCGAAGCGTGGGATATCCACCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGtt < 2:1036808/150‑1 (MQ=255) aaGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGttt < 2:274156/150‑1 (MQ=255) gCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCt > 2:1524323/1‑150 (MQ=255) gCCCGGTTCCAAGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCCTGCCGGGGTAATCACCTTGTCAGGTGGTGGATTTGTTTCt > 2:1668521/1‑150 (MQ=255) cccGGTTCCAAGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAgtgtgt < 1:487121/137‑1 (MQ=255) cccGGTTCCAAGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAgtgtgt > 2:487121/1‑137 (MQ=255) cccGGTTCCAAGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCtt > 2:1541066/1‑150 (MQ=255) cGGTTCCTTGGAACCGGGCTTTTCTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTAc < 2:452295/150‑1 (MQ=255) cGGTTCCAAGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTAc < 1:1541066/150‑1 (MQ=255) ggTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTAcc > 2:335825/1‑150 (MQ=255) ttGGACCCGGGCTTTTTTTTTAGCCTTGAACTAAACTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGACg < 2:202957/150‑1 (MQ=255) ttGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCa < 1:566936/64‑1 (MQ=255) ttGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCa > 2:566936/1‑64 (MQ=255) ttGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGtt < 2:1040216/137‑1 (MQ=255) ttGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGtt > 1:1040216/1‑137 (MQ=255) ttGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGACg > 1:24825/1‑150 (MQ=255) | CTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGACG > NC_002947/1503929‑1504225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |