Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,509,444 C→A 6.4% Q106H (CAG→CAT)  PP_1324 ← endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,509,4440CA 6.4% 188.9 / 2.5 63Q106H (CAG→CAT) PP_1324endonuclease
Reads supporting (aligned to +/- strand):  ref base C (30/28);  new base A (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

TCCATCTGGTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCG  >  NC_002947/1509296‑1509593
                                                                                                                                                    |                                                                                                                                                     
tcaaTCTGGTAACTTTAAAGGGGGCGGGGGCCTTTGCTACGCCAAACAACGACAAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCt                                                                                                                                                      <  2:1348337/147‑1 (MQ=255)
   aTCTGGTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCa                                                                                                                                                   >  1:73757/1‑150 (MQ=255)
   aTCTGGTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCa                                                                                                                                                   <  2:1704748/150‑1 (MQ=255)
    tCTGGTGACCTTAAATGGGGCGGTAGCCTTTGCTACGCGCAACAACGCCCAGCCCGCGATCTAGAGAAGGTAGAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCAGGGTAGCCACCATGCAg                                                                                                                                                  <  1:526714/150‑1 (MQ=255)
          gACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGAc                                                                                                                                            >  1:817182/1‑150 (MQ=255)
          gACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGAc                                                                                                                                            <  1:1673331/150‑1 (MQ=255)
            ccTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTGGTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCa                                                                                                                                          >  1:1166615/1‑150 (MQ=255)
             cTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCAc                                                                                                                                         >  1:838037/1‑150 (MQ=255)
              ttAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACg                                                                                                                                        >  2:682621/1‑150 (MQ=255)
                             gCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGC‑AAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGt                                                                                                                        >  1:1374234/1‑150 (MQ=255)
                               cTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTa                                                                                                                       >  1:1503795/1‑150 (MQ=255)
                                   gCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTg                                                                                                                   <  2:1533365/150‑1 (MQ=255)
                                        gcgcAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAg                                                                                                                            <  2:1710486/136‑1 (MQ=255)
                                        gcgcAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAg                                                                                                                            >  1:1710486/1‑136 (MQ=255)
                                         cgcAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAg                                                                                                             <  2:1503795/150‑1 (MQ=255)
                                          acaacaaCGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGt                                                                                                            <  1:677028/149‑1 (MQ=255)
                                            aacaacGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTgg                                                                                                          >  1:418229/1‑150 (MQ=255)
                                             acaacGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTggg                                                                                                         <  1:315448/150‑1 (MQ=255)
                                              caacCACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGc                                                                                                        <  1:1380936/150‑1 (MQ=255)
                                               aacGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGcc                                                                                                       >  1:751833/1‑150 (MQ=255)
                                               aacGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGcc                                                                                                       >  1:1378239/1‑150 (MQ=255)
                                                  gACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGcctcc                                                                                                    <  2:1842231/150‑1 (MQ=255)
                                                     cAGCCGGCGATACAAAGGGCAAAAAGGCCGGAGTGGTTTCAGCGTTGGAAGGGGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTc                                                                                                 <  1:1884195/150‑1 (MQ=255)
                                                     cAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTc                                                                                                 <  1:192497/150‑1 (MQ=255)
                                                                      agCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTggc                                                                                <  1:48423/150‑1 (MQ=255)
                                                                        cAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTggcgg                                                                              >  2:773571/1‑150 (MQ=255)
                                                                          aaaaaaaCGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAg                                                                                                                            <  2:1049666/102‑1 (MQ=255)
                                                                          aaaaaaaCGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAg                                                                                                                            >  1:1049666/1‑102 (MQ=255)
                                                                              aaaCGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCa                                                                        >  2:1428991/1‑150 (MQ=255)
                                                                              aaaCGAAGTGGGTTCAGCATTCGAAGACGTTTTTCAGCCATTGAAGCGCTCTGCCTGCATGGTGGCTACCCTGCGGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGTGCTCCTACTGCAAGAACAGGTTGGCGGCaacct                                                                        >  2:251822/1‑147 (MQ=255)
                                                                                     gTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCg                                                                 >  1:1520182/1‑150 (MQ=255)
                                                                                      tGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGt                                                                >  1:377646/1‑150 (MQ=255)
                                                                                        ggTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCCAGTACCAACCGttt                                                              <  2:73757/150‑1 (MQ=255)
                                                                                          ttCAGCATTCGAAGGCGTTTTTCTTCTTTTTAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTTGCGGCAAGCACCAACCGTTTCt                                                            >  2:97056/1‑150 (MQ=255)
                                                                                              gCATTCGAAGGCGTTTTTCAGCCATTGAAGCCGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCtt                                                        <  1:413315/150‑1 (MQ=255)
                                                                                               cATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGc                                                                                                                  >  2:584275/1‑91 (MQ=255)
                                                                                               cATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGc                                                                                                                  <  1:584275/91‑1 (MQ=255)
                                                                                                aTTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCACTGACTTGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCATTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTgc                                                      <  1:1477900/150‑1 (MQ=255)
                                                                                                 ttCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTgcg                                                     >  1:293254/1‑150 (MQ=255)
                                                                                                     aaGGCGTTTTTCAGCCCTTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCg                                                 >  2:163155/1‑150 (MQ=255)
                                                                                                        gCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCaa                                                                           >  1:276997/1‑121 (MQ=255)
                                                                                                        gCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCaa                                                                           <  2:276997/121‑1 (MQ=255)
                                                                                                        gCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCAGGGTAGCCACCATGCAgg                                                                                                                                                 <  1:1463641/51‑1 (MQ=18)
                                                                                                        gCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCAGGGTAGCCACCATGCAgg                                                                                                                                                 >  2:1463641/1‑51 (MQ=18)
                                                                                                          gTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGAt                                            >  1:69368/1‑150 (MQ=255)
                                                                                                          gTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGAt                                            >  1:996169/1‑150 (MQ=255)
                                                                                                           tttttCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATa                                           <  2:1378239/150‑1 (MQ=255)
                                                                                                                cAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTc                                      <  1:103002/150‑1 (MQ=255)
                                                                                                                 aGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAAcc                                                                  >  2:1714539/1‑121 (MQ=255)
                                                                                                                 aGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAAcc                                                                  <  1:1714539/121‑1 (MQ=255)
                                                                                                                  gCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCga                                    <  2:1374234/150‑1 (MQ=255)
                                                                                                                     aTTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGagag                                 <  2:293254/150‑1 (MQ=255)
                                                                                                                      ttGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCgag                                   <  1:803289/147‑1 (MQ=255)
                                                                                                                      ttGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCgag                                   >  2:803289/1‑147 (MQ=255)
                                                                                                                      ttGAAGCGGTCTGCCTGCATGGAGGCTCAGTTGCAGGACGAGCACGTCGAGGTGGCAGGGCTAGTTGCCCGAGTGGGCCTCCTTCTGTAGCAACAGGGTGGGGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCAATACTGGCgcgcgc                                >  2:1730502/1‑145 (MQ=255)
                                                                                                                           gCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTc                           >  1:635281/1‑150 (MQ=255)
                                                                                                                              gtcgccctgcAGGGTAGCCACCATGCAGGCAGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGACAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGa                        >  2:1700465/6‑150 (MQ=255)
                                                                                                                              gTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGa                        >  1:816553/1‑150 (MQ=255)
                                                                                                                               tCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCTGTCGATACTGTCGAGAGCGCCTCCGaa                       <  1:773571/150‑1 (MQ=255)
                                                                                                                                   cctgcATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGCCGGCAAGCACCAACGGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGccc                   >  1:33467/1‑150 (MQ=255)
                                                                                                                                    ctgcATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGATAGCGCCTCCGAAGCCcg                  >  1:409559/1‑150 (MQ=255)
                                                                                                                                       cATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGt               <  2:838037/150‑1 (MQ=255)
                                                                                                                                            tggCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAAc          <  1:682621/150‑1 (MQ=255)
                                                                                                                                                   ccTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTc                                      >  2:1356771/1‑115 (MQ=255)
                                                                                                                                                   ccTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTc                                      <  1:1356771/115‑1 (MQ=255)
                                                                                                                                                    cTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCg  >  2:107499/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCATCTGGTGACCTTAAATGGGGCGGTGGCCTTTGCTACGCGCAACAACGACCAGCCCGCGATACATAGAGCAAAAAAAACGAAGTGGGTTCAGCATTCGAAGGCGTTTTTCAGCCATTGAAGCGGTCTGCCTGCATGGTGGCTACCCTGCAGGGCGACCACGTCGAAGCGGCAGGGGTAGTTGCCCCAGTGGGCCTCCTTCTGCAGGAACAGGGTGGCGGCAAGCACCAACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCG  >  NC_002947/1509296‑1509593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑