Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,683,293 C→G 6.9% intergenic (+60/‑171) recJ → / → PP_1478 ssDNA‑specific exonuclease RecJ/xenobiotic reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,683,2930CG 6.9% 160.1 / 2.6 52intergenic (+60/‑171)recJ/PP_1478ssDNA‑specific exonuclease RecJ/xenobiotic reductase
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base G (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGC  >  NC_002947/1683147‑1683429
                                                                                                                                                  |                                                                                                                                        
gTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCcgcgcg                                                                                                                                       <  2:743911/150‑1 (MQ=255)
 tGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGGGTTTAGCGGCAGGTGCCTGCCTGGGATGTTGCGTTGGGGCACAGATCGGGCGGCGCCCCCgcgg                                                                                                                                      >  2:408372/1‑150 (MQ=255)
  ggAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCgcggc                                                                                                                                     <  2:720415/150‑1 (MQ=255)
      ttGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                 >  1:1766780/1‑150 (MQ=255)
                           aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc                                                                                                            >  1:892058/1‑150 (MQ=255)
                           aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc                                                                                                            >  2:275568/1‑150 (MQ=255)
                           aaCGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCGGCAAGCGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc                                                                                                            >  1:1304740/1‑150 (MQ=39)
                                 tttCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCg                                                                                                      <  1:1454496/150‑1 (MQ=255)
                                                 gtggGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCAt                                                                                      <  2:1078201/150‑1 (MQ=255)
                                                     gCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCca                                                                                  <  1:1345707/150‑1 (MQ=255)
                                                      cAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGTCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCACTGCAAACCTTTCGGCGAACCCCTCGCCATCCcac                                                                                 >  2:606411/1‑150 (MQ=255)
                                                         ttGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGa                                                                              >  2:1626391/1‑150 (MQ=255)
                                                             tgatTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCt                                                                          >  1:114914/1‑150 (MQ=255)
                                                              gatTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGACATCCCACAGAACCTc                                                                         <  2:1304740/150‑1 (MQ=255)
                                                                 tGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTcccc                                                                      <  2:869951/150‑1 (MQ=255)
                                                                     cATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCccac                                                                  <  2:892058/150‑1 (MQ=255)
                                                                            aGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTg                                                           <  1:1626391/150‑1 (MQ=255)
                                                                              ccGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGt                                                         <  2:1878912/150‑1 (MQ=255)
                                                                                gcgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca                                                                   <  2:262193/138‑1 (MQ=255)
                                                                                gcgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca                                                                   >  1:262193/1‑138 (MQ=255)
                                                                                 cgcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTa                                                      >  2:1362904/1‑150 (MQ=255)
                                                                                  gcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAg                                                     <  1:275568/150‑1 (MQ=255)
                                                                                        tttAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCAt                                               >  2:1525024/1‑150 (MQ=255)
                                                                                               tCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAGTGAc                                        >  1:1442298/1‑150 (MQ=255)
                                                                                               tCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAc                                        <  2:114914/150‑1 (MQ=255)
                                                                                                 aGGTCCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCg                                      <  1:408372/150‑1 (MQ=255)
                                                                                                   gTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGc                                    >  1:1158249/1‑150 (MQ=255)
                                                                                                        tgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCgg                               <  2:1766780/150‑1 (MQ=255)
                                                                                                        tgcctTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCAAAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCgg                               <  1:636700/150‑1 (MQ=255)
                                                                                                            ttGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc                                                                                                            <  1:796985/69‑1 (MQ=255)
                                                                                                            ttGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAAc                                                                                                            >  2:796985/1‑69 (MQ=255)
                                                                                                            ttGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCa                           >  2:904950/1‑150 (MQ=255)
                                                                                                               gATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAc                                        >  2:1812358/1‑134 (MQ=255)
                                                                                                               gATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAc                                        <  1:1812358/134‑1 (MQ=255)
                                                                                                                 tCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt                      <  1:1370452/150‑1 (MQ=255)
                                                                                                                           gggCACAAATCGAGGGCGTCCCGCGCGGGGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGCACGCCTGGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa            <  1:119676/150‑1 (MQ=255)
                                                                                                                           gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa            <  2:1158249/150‑1 (MQ=255)
                                                                                                                             gCACAAATCGAGCGCCGCCCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAgt          >  1:1799295/1‑150 (MQ=255)
                                                                                                                             ccgatcttCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAgt          <  1:901233/143‑1 (MQ=255)
                                                                                                                              cacaAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt                      <  1:353836/137‑1 (MQ=255)
                                                                                                                              cacaAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt                      >  2:353836/1‑137 (MQ=255)
                                                                                                                               acaAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAcc                                                                           <  1:310285/83‑1 (MQ=255)
                                                                                                                               acaAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAAcc                                                                           >  2:310285/1‑83 (MQ=255)
                                                                                                                                 aaaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTcc      >  2:579679/1‑150 (MQ=255)
                                                                                                                                  gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGACCCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAcc                                       <  2:1140770/115‑1 (MQ=38)
                                                                                                                                  gaTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGAcc                                       >  1:1140770/2‑116 (MQ=255)
                                                                                                                                  aaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCg     <  1:1525024/150‑1 (MQ=255)
                                                                                                                                   aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTACCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCgg    >  1:1277148/1‑150 (MQ=255)
                                                                                                                                    tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTccc                                                                       >  1:1883575/1‑82 (MQ=255)
                                                                                                                                    tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTccc                                                                       <  2:1883575/82‑1 (MQ=255)
                                                                                                                                    tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCggg   >  1:240803/1‑150 (MQ=255)
                                                                                                                                    ctgAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCggg   <  1:1380868/148‑1 (MQ=255)
                                                                                                                                     cGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGCTGTCTACTCGAgtgagaccgg  >  2:901233/1‑143 (MQ=39)
                                                                                                                                                  |                                                                                                                                        
GTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGC  >  NC_002947/1683147‑1683429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑