Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,903,568 G→C 9.4% intergenic (+51/‑220) PP_1703 → / → nirB assimilatory nitrate reductase/sulfite reductase/nitrite reductase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,903,5680GC 9.4% 191.7 / 9.0 63intergenic (+51/‑220)PP_1703/nirBassimilatory nitrate reductase/sulfite reductase/nitrite reductase large subunit
Reads supporting (aligned to +/- strand):  ref base G (30/27);  new base C (3/3);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTGTCGCCGAGCATGGCCGGATGGATGCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACC  >  NC_002947/1903423‑1903717
                                                                                                                                                 |                                                                                                                                                     
ttGTCGCCGAGCATGGCCGGATGGATGCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGccc                                                                                                                                                   >  1:1931734/1‑150 (MQ=255)
   tCGCCGAGCATGGCCGGATGGATGCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTgggcgggcatagc                                                                                                                                                <  1:1894378/150‑6 (MQ=255)
       cGAGCATGGCCGGATGGATGCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCCCGCCCAgccaat                                                                                                                                            <  1:231510/150‑5 (MQ=255)
             gggCCGGATGGATGCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAg                                                                                                                                      <  2:109620/149‑1 (MQ=255)
                   gatggatgCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCgg                                                                                                                                <  2:1864938/150‑1 (MQ=255)
                   gatggatgCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCgg                                                                                                                                <  2:215242/150‑1 (MQ=255)
                     gggaggCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGt                                                                                                                              <  1:812704/149‑1 (MQ=255)
                                     tgcggctTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCCCGCCCAgccaatgc                                                                                                                                          <  2:1835191/122‑7 (MQ=255)
                                     tgcggctTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCCCGCCCAgccaatgc                                                                                                                                          >  1:1835191/1‑116 (MQ=255)
                                            tATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGAcaca                                                                                                       <  2:1931734/150‑1 (MQ=255)
                                                   aaGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGa                                                                                                           <  2:1774969/139‑1 (MQ=255)
                                                   aaGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGa                                                                                                           >  1:1774969/1‑139 (MQ=255)
                                                   aaGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcaggcaacc                                                                                                >  2:13107/1‑150 (MQ=255)
                                                      gCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGcc                                                                                                             <  2:571949/134‑1 (MQ=255)
                                                      gCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGcc                                                                                                             >  1:571949/1‑134 (MQ=255)
                                                      gCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcaggcaaccca                                                                                              <  1:152768/149‑1 (MQ=255)
                                                      gCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcaggcaaccca                                                                                              >  2:152768/1‑149 (MQ=255)
                                                        ttGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGGTTACCCGCGAAGAGGCCGACAcaggcaacccgggc                                                                                           >  1:189539/1‑150 (MQ=255)
                                                             cAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGaa                                                                                                                   >  1:1722936/1‑121 (MQ=255)
                                                             cAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGaa                                                                                                                   <  2:1722936/121‑1 (MQ=255)
                                                              aaGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAggcaacccaggcaaccca                                                                                     >  2:1655300/1‑150 (MQ=255)
                                                                         ttATCGGCGCGATGTGTACTGAAGTGCTAGAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGAt                                                                          <  1:1830471/150‑1 (MQ=255)
                                                                            tCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcag                                                                                                      >  1:750168/1‑119 (MQ=255)
                                                                            tCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcag                                                                                                      <  2:750168/119‑1 (MQ=255)
                                                                            tCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCGTGAATGTGCATTGTCTGGGCGGGCGCAGCGCAAGTGTAAGAACGGGTTTACCCGCGACGAGGCCGACACAGGCAACCCAGGCAACCCGCGCAACCCGATTgg                                                                       >  1:1150379/1‑150 (MQ=255)
                                                                              ggCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCa                                                                     >  1:569744/1‑150 (MQ=255)
                                                                               gcgcGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCAc                                                                    >  1:1065136/1‑150 (MQ=255)
                                                                               gcgcGATGTGTACTGAAGTGCGCCAAGGTGGGGGGTTGTGCACCTTGTATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGCGACCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCAc                                                                    >  2:1574524/1‑150 (MQ=255)
                                                                                    aTGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGACCAGACTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGGAACCCAGGCAACCCACGCAACCCGATTGGCACGCTcc                                                               <  1:1574524/150‑1 (MQ=255)
                                                                                       tgtACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGAcaca                                                                                                       >  2:1345458/1‑107 (MQ=255)
                                                                                       tgtACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGAcaca                                                                                                       <  1:1345458/107‑1 (MQ=255)
                                                                                       tgtACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTg                                                            <  2:1785536/150‑1 (MQ=255)
                                                                                         tACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcaggcaacccaggc                                                                                           <  1:716068/117‑1 (MQ=255)
                                                                                         tACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACAcaggcaacccaggc                                                                                           >  2:716068/1‑117 (MQ=255)
                                                                                          aCTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAAccc                                                                             >  2:1243823/1‑130 (MQ=255)
                                                                                          aCTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAAccc                                                                             <  1:1243823/130‑1 (MQ=255)
                                                                                           cTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTa                                                        >  1:506504/1‑150 (MQ=255)
                                                                                            tGAAGTGCTCCAAGATGGGGTGTTTTGCACCGTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTaa                                                       >  2:172030/1‑150 (MQ=255)
                                                                                               aGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCt                                                    >  2:1896244/1‑150 (MQ=255)
                                                                                                gTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTc                                                   <  1:899975/150‑1 (MQ=255)
                                                                                                   cTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGa                                                <  2:506504/150‑1 (MQ=255)
                                                                                                   cTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGa                                                <  1:1009619/150‑1 (MQ=255)
                                                                                                      cAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCaa                                                                                >  1:810269/1‑115 (MQ=255)
                                                                                                      cAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCaa                                                                                <  2:810269/115‑1 (MQ=255)
                                                                                                      cAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATac                                             >  2:1194794/1‑150 (MQ=255)
                                                                                                           tGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATAcaacaa                                        >  2:886910/1‑150 (MQ=255)
                                                                                                              ggTGTTGTGCACCTTGTATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAg                                                                                                                                      >  1:559184/1‑53 (MQ=255)
                                                                                                              ggTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAg                                                                                                                                      <  2:559184/53‑1 (MQ=255)
                                                                                                               gtgtTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGcc                                    >  1:854422/1‑150 (MQ=255)
                                                                                                                 gttgtGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCa                                  <  1:13107/150‑1 (MQ=255)
                                                                                                                 gttgtGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCa                                  >  1:674126/1‑150 (MQ=255)
                                                                                                                  ttgtGCACCTTGAATGTGCATTGGCTGGGTGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                              <  2:1404699/73‑1 (MQ=255)
                                                                                                                  ttgtGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                              >  1:1404699/1‑73 (MQ=255)
                                                                                                                     tgCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGa                              <  1:1194794/150‑1 (MQ=255)
                                                                                                                       cACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATc                            <  2:1065136/150‑1 (MQ=255)
                                                                                                                          cTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAAc                         >  1:1003093/1‑150 (MQ=255)
                                                                                                                           ttGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACg                        >  2:485496/1‑150 (MQ=255)
                                                                                                                                 gtgCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATc                  >  1:1410436/1‑150 (MQ=255)
                                                                                                                                    cATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAAc               <  1:886910/150‑1 (MQ=255)
                                                                                                                                     atgggCTGGGCGGGCCCAGCGCAAGTGTAGTAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGATCACTGGTTAAGATAGCATAAGATAGACGCCACGGAACAACGGCGATCAAcc              >  2:1181420/4‑150 (MQ=25)
                                                                                                                                      tgcgCTGGGCCCGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCg             >  2:949902/4‑150 (MQ=255)
                                                                                                                                         gCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGc          <  1:199691/150‑1 (MQ=255)
                                                                                                                                         gCTGGGCCCGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCa                                  <  1:1793955/126‑1 (MQ=255)
                                                                                                                                         gCTGGGCCCGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCa                                  >  2:1793955/1‑126 (MQ=255)
                                                                                                                                               cgggcCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTa    <  2:1410436/150‑1 (MQ=255)
                                                                                                                                                 ggcCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTccc  <  2:1150379/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TTGTCGCCGAGCATGGCCGGATGGATGCGGCGGCGGCTGCGGCTTATGTCGAAGGCTTGAGCAAGGCCAGGCGTTATCGGCGCGATGTGTACTGAAGTGCTCCAAGATGGGGTGTTGTGCACCTTGAATGTGCATTGGCTGGGCGGGCCCAGCGCAAGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGACACAGGCAACCCAGGCAACCCACGCAACCCGATTGGCACGCTCCCTGCTTAACCTCGGATACAACAAAGCCCACTGATCAACGGCGATCAACCGGGCCCCCTACC  >  NC_002947/1903423‑1903717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑