Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,013,746 C→A 7.0% A107S (GCA→TCA)  PP_1793 ← group 2 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,013,7460CA 7.0% 175.7 / 3.2 57A107S (GCA→TCA) PP_1793group 2 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base A (2/2);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

TGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCAGTTTGCATGGCGCGCTCA  >  NC_002947/2013600‑2013888
                                                                                                                                                  |                                                                                                                                              
tgtgGCGGGGGGCTCGGACTGCCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCTCGACCTACCAGTGCCTg                                                                                                                                             <  2:995598/150‑1 (MQ=255)
    gCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACa                                                                                                                                         >  1:464042/1‑150 (MQ=255)
            cTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGc                                                                                                                                                <  2:1444328/135‑1 (MQ=255)
            cTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGc                                                                                                                                                >  1:1444328/1‑135 (MQ=255)
            cTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCTTGGa                                                                                                                                           >  2:354922/1‑140 (MQ=255)
            cTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGa                                                                                                                                           <  1:354922/140‑1 (MQ=255)
               ggACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTc                                                                                                                              >  2:912090/1‑150 (MQ=255)
                   tGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCgg                                                                                                                           <  2:123527/149‑1 (MQ=255)
                   tGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCgg                                                                                                                           >  1:123527/1‑149 (MQ=255)
                   tGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGt                                                                                                                          >  1:530954/1‑150 (MQ=255)
                       tACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCGATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACa                                                                                                                      <  1:1877247/150‑1 (MQ=255)
                       tACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACa                                                                                                                      >  1:1449904/1‑150 (MQ=255)
                       tACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACa                                                                                                                      >  2:1042114/1‑150 (MQ=255)
                                    aaGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGtt                                                                                                         <  1:929423/150‑1 (MQ=255)
                                     agcagcATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTg                                                                                                        >  2:358031/1‑150 (MQ=255)
                                     agcagcATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTg                                                                                                        <  1:1042114/150‑1 (MQ=255)
                                            tACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGa                                                                                                 <  2:530954/150‑1 (MQ=255)
                                                cAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATaa                                                                                             <  1:1360870/150‑1 (MQ=255)
                                                 aaGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCa                                                                                                                                      >  2:1064679/1‑108 (MQ=255)
                                                 aaGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCa                                                                                                                                      <  1:1064679/108‑1 (MQ=255)
                                                         gTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGACTGGACAt                                                                                                                                        >  2:962536/1‑98 (MQ=255)
                                                         gTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGACTGGACAt                                                                                                                                        >  2:962509/1‑98 (MQ=255)
                                                         gTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGACTGGACAt                                                                                                                                        <  1:962509/98‑1 (MQ=255)
                                                         gTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGACTGGACAt                                                                                                                                        <  1:962536/98‑1 (MQ=255)
                                                                            cATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAAt                                                                 >  1:556055/1‑150 (MQ=255)
                                                                            cATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAAt                                                                 <  2:1680272/150‑1 (MQ=255)
                                                                                ccTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTg                                                                                                                                             >  1:206415/1‑70 (MQ=255)
                                                                                ccTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTg                                                                                                                                             <  2:206415/70‑1 (MQ=255)
                                                                                     gTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGAtt                                                                           >  2:488561/1‑131 (MQ=255)
                                                                                     gTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGAtt                                                                           <  1:488561/131‑1 (MQ=255)
                                                                                            aTCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTcc                                                 >  2:1170144/1‑150 (MQ=255)
                                                                                               gggTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTgc                                                                       >  1:1596511/1‑125 (MQ=255)
                                                                                               gggTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTgc                                                                       <  2:1596511/125‑1 (MQ=255)
                                                                                                 gTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTc                                                  <  2:1707680/144‑1 (MQ=255)
                                                                                                 gTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTc                                                  >  1:1707680/1‑144 (MQ=255)
                                                                                                       aGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTaa                                                                                                                        <  2:13004/68‑1 (MQ=255)
                                                                                                       aGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTaa                                                                                                                        >  1:13004/1‑68 (MQ=255)
                                                                                                        gCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTAGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCct                                     >  1:1607884/1‑150 (MQ=255)
                                                                                                          aCTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCctct                                   >  2:1298712/1‑150 (MQ=255)
                                                                                                            tGCAGGCCCTTGTGCCAGCAAGGCGCGACCTATCCGTGCCTGGACATCAGCCCGGATCGGTAACAAGTGGGCCGCGTTGATAAAGAATAACAATGCCAGCTTAGGATTGTGCGATAATGCCGCCATACCTTTCCATACCAGTGCCTCTgg                                 >  2:595676/1‑150 (MQ=255)
                                                                                                            tGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCAt                                                         >  2:1766781/1‑126 (MQ=255)
                                                                                                            tGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCAt                                                         <  1:1766781/126‑1 (MQ=255)
                                                                                                            tGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTgg                                 >  1:1758588/1‑150 (MQ=255)
                                                                                                            tGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGGTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGACGCCATGCCTTTCCCTACCCGTGCCTCTgg                                 >  2:7492/1‑150 (MQ=255)
                                                                                                             gCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGc                                <  2:1758588/150‑1 (MQ=255)
                                                                                                               aaGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCg                              <  1:358031/150‑1 (MQ=255)
                                                                                                                 gCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGt                            >  2:1940609/1‑150 (MQ=255)
                                                                                                                 gCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGt                            <  2:1128136/150‑1 (MQ=255)
                                                                                                                     ttGTGCCAGCAAGGCGCGACCTAGCAGGGCCTGGACATCAGCACGGACCGGGAACAATTGGGCTGCGTTAATAAAGAATAAAAATGCCAGAATAGGCTTGTGCGCTAATGCTGCCATACCTTTCCATACCAGGACATCTGCCCGGTGCTc                        >  2:188406/1‑150 (MQ=255)
                                                                                                                     ttGTGCCAGCAAGGCGCGACCTACCAGTGCCTAGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTc                        <  2:1607884/150‑1 (MQ=255)
                                                                                                                            aGCAAGGCGCGACCTACCAGAGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCAGtt                 <  1:912090/150‑1 (MQ=255)
                                                                                                                               aaGGCGCGACCTACCACAGCATGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCAGTTTGc              >  2:1928349/1‑150 (MQ=255)
                                                                                                                               aaGGCGCGACCCACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGa                                                                             >  2:860977/1‑87 (MQ=255)
                                                                                                                               aaGGCGCGACCCACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGa                                                                             <  1:860977/87‑1 (MQ=255)
                                                                                                                                  gcgcGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCAGTTTGCATg           <  2:780066/150‑1 (MQ=255)
                                                                                                                                    gcgACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCCATTTGCATGgc         >  1:1782184/1‑150 (MQ=255)
                                                                                                                                           aCCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCAGTTTGCATGGCGCGCTCa  >  1:1688656/1‑150 (MQ=255)
                                                                                                                                             cAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCttt                                                   <  1:491641/99‑1 (MQ=255)
                                                                                                                                             cAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCttt                                                   >  2:491641/1‑99 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TGTGGCGGGGGGCTCGGACTGGCTACGTGCCTGCCAAAGCAGCATACGCAAGGAGGCGTCATCGGGTAGTTTCTGCCATGCCTGCGTGAGGTATCGGGTGGCGAGCACTGCAAGCCCTTGTGCCAGCAAGGCGCGACCTACCAGTGCCTGGACATCAGCACGGTCCGGTAACAATTGGGCTGCGTTGATAAAGAATAAAAATGCCAGCTTAGGATTGTGCGCTAATGCCGCCATGCCTTTCCATACCAGTGCCTCTGGCCGGTGCTCGGCAGTTTGCATGGCGCGCTCA  >  NC_002947/2013600‑2013888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑