Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,244 G→A 5.0% intergenic (+58/‑149) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2440GA 5.0% 275.7 / 2.1 93intergenic (+58/‑149)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (46/42);  new base A (2/3);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCA  >  NC_002947/2063097‑2063392
                                                                                                                                                   |                                                                                                                                                     
aTGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAggg                                                                                                                                                     <  1:507213/150‑1 (MQ=255)
    tCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCg                                                                                                                                                 >  1:1393982/1‑150 (MQ=255)
      aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑a                                                                                                                                              <  1:387473/150‑1 (MQ=255)
             tGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTtgtg                                                                                                                                       <  1:1503954/150‑1 (MQ=255)
              ggAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGc                                                                                                                                      >  1:1349889/1‑150 (MQ=255)
               gAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAgg                                                                                                                                                      >  1:1183042/1‑134 (MQ=255)
               gAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAgg                                                                                                                                                      <  2:1183042/134‑1 (MQ=255)
                aaGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCACGCCCCCAGAGGCCTTGCAC‑GGGCTCGGCAGCTTGTGCAg                                                                                                                                    >  2:1277292/1‑150 (MQ=255)
                 aGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCTGCTCCCACAGGCATTGCACAAGGTCCGA‑ACCCTGTGCaat                                                                                                                                   <  1:1852968/150‑3 (MQ=38)
                   cGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGACCACGCACAGGGTTCGG‑ACCTTGTGCAGGTc                                                                                                                                 <  1:1322240/150‑1 (MQ=255)
                    gTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTcc                                                                                                                                >  1:547265/1‑150 (MQ=255)
                         tCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGc                                                                                                                           <  1:1447071/150‑1 (MQ=255)
                            aGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACACACGCTGCACAAGGTCCGA‑ACCCTGTGCAATGCCTGTGGGAg                                                                                                                        >  1:18755/1‑150 (MQ=21)
                                   gACGAAGATCCTGCTGGATAACACCGCCGGGGTGATGCACGGCACACCGAAGTAAACCGGGGCCGCGTTACGGCCCATTCGCGGGCTTGCCCGCTCGCACAGGACCTGCACAAGGTCCGA‑ACCCTGTGCAATGCCTGTGGGAGCGGGCaa                                                                                                                 <  2:283229/150‑1 (MQ=14)
                                     ccAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGTACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGc                                                                                                               >  2:1619620/1‑150 (MQ=255)
                                        agagCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCcg                                                                                                            >  2:707844/1‑150 (MQ=255)
                                            ccTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCaa                                                                                                                 >  1:1856215/1‑141 (MQ=255)
                                            ccTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCaa                                                                                                                 <  2:1856215/141‑1 (MQ=255)
                                              tgctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                      <  1:1501838/150‑1 (MQ=255)
                                              tgctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                      >  1:385670/1‑150 (MQ=255)
                                              tgctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCGGGTCGGCACTCAGAGGCATTGCACAGGGTTCGG‑AACTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                                      >  2:557918/1‑150 (MQ=255)
                                               gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAAGGTCCGA‑Acc                                                                                                                                            <  2:469969/111‑1 (MQ=255)
                                                  cgatctcacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGcc                                                                                                  <  1:1384679/144‑1 (MQ=255)
                                                      aaCACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGCGCGGGCAAGCCCGCGAATGGGcgccgc                                                                                              >  1:1734571/1‑150 (MQ=255)
                                                         acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTATGGGAGCGGGGAAGCCCGCGAAGGGGcccagatcg                                                                                           >  2:1384679/1‑144 (MQ=38)
                                                          ccgccgGCGTGCGACACGGCACACCGAAGTAAACCGGCGCCGCTTTGCGCCCCATTCGCGGGCTTCCCCGCGCCCACGGCCATTGCACGGGGTTCGG‑CCTTTGGGCGGGTCCTGTGCGAGCGGGCTAGCCCGCGAATGGGCCCCGCACaa                                                                                          <  1:557918/150‑1 (MQ=255)
                                                           cgccgGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAAt                                                                                         <  1:1760161/150‑1 (MQ=255)
                                                            gccgGGGTGGGACACGGCACACCGAAGTAAGCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCGACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGGGCGAGCGGGCAAGCCCGCGAATTGGCCCCGCACAAtt                                                                                        <  2:1734571/150‑1 (MQ=38)
                                                                      tgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                              <  2:385670/150‑1 (MQ=255)
                                                                      tgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                              <  1:1202254/150‑1 (MQ=255)
                                                                      tgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACt                                                                              >  1:531917/1‑150 (MQ=255)
                                                                         aCGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCCCAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGaa                                                                           <  2:547265/150‑1 (MQ=255)
                                                                          cGGTACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGCCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAAc                                                                          <  1:1619620/150‑1 (MQ=255)
                                                                           ggCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCTGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACa                                                                         >  2:1603515/1‑150 (MQ=255)
                                                                                  cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCCAGGCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATccc                                                                  >  1:701722/1‑150 (MQ=255)
                                                                                   gAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTCTcccc                                                                 <  2:1349889/150‑1 (MQ=255)
                                                                                    aaGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                                <  1:673750/134‑1 (MQ=255)
                                                                                    aaGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGa                                                                                >  2:673750/1‑134 (MQ=255)
                                                                                      gTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGcc                                                              >  2:1655783/1‑150 (MQ=255)
                                                                                         aaCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt                                                                                                       >  1:663874/1‑106 (MQ=255)
                                                                                         aaCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAAt                                                                                                       <  2:663874/106‑1 (MQ=255)
                                                                                         aaCCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCa                                                           >  1:273859/1‑150 (MQ=255)
                                                                                            cGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAgg                                                        >  2:371517/1‑150 (MQ=255)
                                                                                                gCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACAtt                                                                       <  1:1061566/131‑1 (MQ=255)
                                                                                                gCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACAtt                                                                       >  2:1061566/1‑131 (MQ=255)
                                                                                                  cgcgTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGAATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGtt                                                  >  1:1848920/1‑150 (MQ=255)
                                                                                                   gcgTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTa                                                 >  1:132081/1‑150 (MQ=255)
                                                                                                     gTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAATCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTAtt                                               <  2:1393982/150‑1 (MQ=255)
                                                                                                     gTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTAtt                                               <  2:1848920/150‑1 (MQ=255)
                                                                                                       tGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGCCCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa                                             <  1:1577418/150‑1 (MQ=255)
                                                                                                        gCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAg                                            <  1:707844/150‑1 (MQ=255)
                                                                                                           gCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCa                                         >  1:464801/1‑150 (MQ=255)
                                                                                                            cccATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCAt                                        >  1:194211/1‑150 (MQ=255)
                                                                                                             ccATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATc                                       <  2:132081/150‑1 (MQ=255)
                                                                                                              cgtccgcgGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTGCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCCAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATca                                      <  1:773386/146‑1 (MQ=255)
                                                                                                               aTTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                     >  2:1690259/1‑150 (MQ=255)
                                                                                                               aTTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                     >  2:1895486/1‑150 (MQ=255)
                                                                                                               aTTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCACGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcac                                     >  2:1098526/1‑150 (MQ=255)
                                                                                                                ttCGGGGGCTTGCCCGCTCCCATAGGCATTGCACAGGGTTCGG‑CCCTTGTGCACGCCGGTTGCAATTGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATcaca                                    <  1:228800/150‑1 (MQ=255)
                                                                                                                ttCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAAc                                                                          <  2:1247512/112‑1 (MQ=255)
                                                                                                                ttCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAAc                                                                          >  1:1247512/1‑112 (MQ=255)
                                                                                                                      ggCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCATGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg                              <  2:35286/150‑1 (MQ=255)
                                                                                                                      ggCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg                              <  2:273859/150‑1 (MQ=255)
                                                                                                                         ttGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtg                           <  2:194211/150‑1 (MQ=255)
                                                                                                                            cccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCACGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTc                        <  1:1690259/150‑1 (MQ=255)
                                                                                                                            cccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGGCAGTTATTCAGGCATCACCCACTTGGTGTTc                        >  1:1430175/1‑150 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACGTTTTGCAGGTCCTGTGCGAGCTGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCg                       <  1:1277292/150‑1 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCgg                                                                                                                     >  2:1533708/1‑56 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCgg                                                                                                                     <  1:1533708/56‑1 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCacacac                                 <  1:980323/140‑1 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCacacac                                 >  2:980323/1‑140 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCg                       >  1:312783/1‑150 (MQ=255)
                                                                                                                             ccGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCg                       >  1:1300107/1‑150 (MQ=255)
                                                                                                                               gCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGtt                     >  1:1842211/1‑150 (MQ=255)
                                                                                                                               gCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGtt                     <  2:531917/150‑1 (MQ=255)
                                                                                                                                   ccACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCCAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGtt                     >  2:869417/1‑146 (MQ=255)
                                                                                                                                   ccACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAg                                                         >  2:1048212/1‑110 (MQ=255)
                                                                                                                                   ccACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAg                                                         <  1:1048212/110‑1 (MQ=255)
                                                                                                                                   ccACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGtt                     <  1:869417/146‑1 (MQ=255)
                                                                                                                                     acaGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga               >  1:752618/1‑150 (MQ=255)
                                                                                                                                      caGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg                              >  1:1067043/1‑134 (MQ=255)
                                                                                                                                      caGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg                              <  2:1067043/134‑1 (MQ=255)
                                                                                                                                         gCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                                   >  1:1585108/1‑78 (MQ=255)
                                                                                                                                         gCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                                   <  2:1585108/78‑1 (MQ=255)
                                                                                                                                            ttGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTc        >  2:737100/1‑150 (MQ=255)
                                                                                                                                             tGCACAAGGTCCGA‑ACCCTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTACCGGACTGAACATGATCCCCGACCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTcc       >  2:852360/1‑150 (MQ=38)
                                                                                                                                              gCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag              <  1:1420137/142‑1 (MQ=255)
                                                                                                                                              gCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTgag              >  2:1420137/1‑142 (MQ=255)
                                                                                                                                               cacaGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                          <  1:476778/129‑1 (MQ=255)
                                                                                                                                               cacaGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgtgt                          >  2:476778/1‑129 (MQ=255)
                                                                                                                                                acaGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt    >  2:175481/1‑150 (MQ=255)
                                                                                                                                                acaGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGAATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGtt    <  1:1424852/150‑1 (MQ=255)
                                                                                                                                                 caGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTc   >  2:1138096/1‑150 (MQ=255)
                                                                                                                                                  aGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATc                                                                    >  1:1758597/1‑84 (MQ=255)
                                                                                                                                                  aGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATc                                                                    <  2:1758597/84‑1 (MQ=255)
                                                                                                                                                  aGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCa  >  1:380515/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATGCTCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGCTTGCCCGCTCCCACAGGCATTGCACAGGGTTCGG‑ACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCA  >  NC_002947/2063097‑2063392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑