Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,138,457 C→A 9.8% R173S (CGC→AGC)  yadG → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,138,4570CA 9.8% 209.4 / 7.9 68R173S (CGC→AGC) yadGABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (30/31);  new base A (4/3);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCA  >  NC_002947/2138315‑2138598
                                                                                                                                              |                                                                                                                                             
ccTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAt                                                                                                                                        >  2:840509/1‑150 (MQ=255)
  tgtgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAtgt                                                                                                                                      <  2:1297148/150‑1 (MQ=255)
    tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                    >  1:1422863/1‑150 (MQ=255)
    ggtgACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                    <  2:1665629/147‑1 (MQ=255)
     gggACAAACGTGATGTGCAGTCGCGTTCGTTGTCAGGCGCTATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGa                                                                                                                                   <  1:1557235/150‑1 (MQ=255)
      ggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTc                                                                                                                                           <  1:796049/141‑1 (MQ=255)
      ggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTc                                                                                                                                           >  2:796049/1‑141 (MQ=255)
        aCAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGCAGCCGCGCCTTATGATTGCCCGCGCATTAATCAACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCt                                                                                                                                <  1:1399604/150‑1 (MQ=255)
          aaaCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                              <  1:1221474/150‑1 (MQ=255)
          aaaCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                              <  2:1696389/150‑1 (MQ=255)
          aaaCGTGATGTGCAGTCGAGTTCGTTGTCCGCCGGTATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGACCCAACCGCCGGGGTGGATACCGAGCTGTGCCGGTCGATGTGGAGCtgc                                                                                                                              <  2:1574585/150‑3 (MQ=255)
            aCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCt                                                                                                                            >  1:1636330/1‑150 (MQ=255)
            aCGTGATGGGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCt                                                                                                                            <  2:674645/150‑1 (MQ=255)
              gTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCa                                                                                                                          <  1:1302031/150‑1 (MQ=255)
                gATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACt                                                                                                                        <  1:45424/150‑1 (MQ=255)
                gATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTAACt                                                                                                                        <  2:1640655/150‑1 (MQ=255)
                   gtgCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAg                                                                                                                     <  1:1781528/150‑1 (MQ=255)
                           gcgTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCa                                                                                                             >  1:317895/1‑150 (MQ=255)
                             gttcgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGa                                                                                                           >  1:1667664/1‑150 (MQ=255)
                              ttcgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGTCTGGTTTGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGaa                                                                                                          <  1:237860/150‑1 (MQ=255)
                              ttcgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGaa                                                                                                          >  1:923268/1‑150 (MQ=255)
                                                 aaGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTc                                                                                       <  2:1231377/150‑1 (MQ=255)
                                                   gcggcgCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTcac                                                                                     <  1:806905/150‑1 (MQ=255)
                                                       cgcCTGATGATTGCCCGCGAATTCATCCAGGTGCCGCGCCTGCTGATCGTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCaccacc                                                                                 <  2:317895/150‑1 (MQ=255)
                                                         ccTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCAccaccca                                                                               >  2:628697/1‑150 (MQ=255)
                                                               gatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCt                                                                         >  1:7514/1‑150 (MQ=255)
                                                               gatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCt                                                                         <  2:1636330/150‑1 (MQ=255)
                                                                   gCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGaa                                                                     <  2:1422863/150‑1 (MQ=255)
                                                                     ccGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAaga                                                                   >  2:713161/1‑150 (MQ=255)
                                                                          cATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTg                                                              <  2:923268/150‑1 (MQ=255)
                                                                           aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCaccacc                                                                                 >  1:1240719/1‑130 (MQ=255)
                                                                           aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCaccacc                                                                                 <  2:1240719/130‑1 (MQ=255)
                                                                           aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAta                                                                                                                                       >  2:256337/1‑75 (MQ=39)
                                                                           aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAta                                                                                                                                       <  1:256337/76‑2 (MQ=39)
                                                                            ttGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATTGTGCTGCGAAGCTTGATGTGGAGCTTCATCACCGAGCTGAACCAGAAGCGCATCACCATCATCCTCACCGCCCACTAGCTGGAAGTGGTTGag                                                            >  2:37898/1‑150 (MQ=255)
                                                                                tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagcagc                                                        >  1:1381281/1‑150 (MQ=255)
                                                                                tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTAACCACCCACTATCTGGAAGAGGCTGagcagc                                                        >  2:1100478/1‑150 (MQ=255)
                                                                                tCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGACCTGAACCAGAAGGGCATCACCATCATCCTAACCACCCACTATCTGGAAGAGGCTGagcagc                                                        >  2:1100477/1‑150 (MQ=255)
                                                                                  cACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtg                                                      >  2:1065215/1‑150 (MQ=255)
                                                                                   aCGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCAGCATTGATCTGGAAGAGGCTGAGCAGCtgt                                                     >  1:492078/1‑150 (MQ=255)
                                                                                        ccGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGt                                                >  1:1748983/1‑150 (MQ=255)
                                                                                           ggcCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAAc                                             >  2:1329553/2‑150 (MQ=255)
                                                                                              ctgctgATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATc                                          <  1:713161/150‑1 (MQ=255)
                                                                                              ctgctgATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATc                                          <  2:7514/150‑1 (MQ=255)
                                                                                               tgctgATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAATTGGCTGAGCAGCTGTGCCGTAACATCg                                         <  1:840509/150‑1 (MQ=255)
                                                                                                   gATACTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcat                                     >  1:297274/1‑150 (MQ=255)
                                                                                                         ggACGAACCCACCGCCGGGGTGGATATCGAGCGGCGCCGCCGGAGGTGGAGGTTCTGCACTGAGCTGAACCAGGAGGGCATCACCATCATTCTCACCACCCACTATCTGGAAGAGTCTGAGCAGCTGTGCCGTCACCTCGGCATCATGGa                               >  2:282766/1‑150 (MQ=255)
                                                                                                           aCGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATAATAATCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGAcc                             >  2:1718115/1‑150 (MQ=255)
                                                                                                             gAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCAt                                                                                                    >  2:899724/1‑77 (MQ=255)
                                                                                                             gAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCAt                                                                                                    <  1:899724/77‑1 (MQ=255)
                                                                                                                   aCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCAcc                     >  1:1258451/1‑150 (MQ=255)
                                                                                                                   aCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCAcc                     >  2:388234/1‑150 (MQ=255)
                                                                                                                        cgGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCa                                                                                                  >  1:1518814/1‑68 (MQ=255)
                                                                                                                        cgGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCa                                                                                                  <  2:1518814/68‑1 (MQ=255)
                                                                                                                           ggTGGGTATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga             <  1:421354/150‑1 (MQ=255)
                                                                                                                           ggTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCCCTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTCGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga             <  2:773507/150‑1 (MQ=255)
                                                                                                                            gTGGATATCGAGCTGCGCCGCTCGATGTTGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCgag            <  2:970152/150‑1 (MQ=255)
                                                                                                                              ccacatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGaa                                                                     >  1:914363/5‑91 (MQ=39)
                                                                                                                              ccacatCGAGCGGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGaa                                                                     <  2:914363/87‑1 (MQ=39)
                                                                                                                               cacatCGAGCGGCGCAGCTCGATATGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTca                                                                                      <  2:685023/70‑1 (MQ=37)
                                                                                                                               cacatCGAGCGGCGCAGCTCGATATGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTca                                                                                      >  1:685023/4‑73 (MQ=37)
                                                                                                                                 tatCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAacac       >  1:1102729/1‑150 (MQ=255)
                                                                                                                                  atCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACAcc      >  1:2958/1‑150 (MQ=255)
                                                                                                                                      aGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGcc                                                  <  1:958437/102‑1 (MQ=255)
                                                                                                                                      aGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGcc                                                  >  2:958437/1‑102 (MQ=255)
                                                                                                                                      aGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCa  >  2:1126103/1‑150 (MQ=255)
                                                                                                                                         tGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcat                                     <  2:1685250/112‑1 (MQ=255)
                                                                                                                                         tGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcat                                     >  1:1685250/1‑112 (MQ=255)
                                                                                                                                              |                                                                                                                                             
CCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGAACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAGCA  >  NC_002947/2138315‑2138598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑