Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,560,507 G→C 8.6% W142C (TGG→TGC) ‡ PP_2243 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,560,5070GC 8.6% 255.3 / 13.2 81W142C (TGG→TGC) ‡PP_2243hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/37);  new base C (3/4);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGA  >  NC_002947/2560363‑2560649
                                                                                                                                                |                                                                                                                                               
cGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCtggctggc                                                                                                                                            <  2:1452971/150‑1 (MQ=255)
cGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCtggctggc                                                                                                                                            >  1:1384659/1‑150 (MQ=255)
cGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGACGAGAAAGCGCATGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCtggctggc                                                                                                                                            <  2:1022279/150‑1 (MQ=255)
  aCCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTggcgg                                                                                                                                          <  1:1740404/150‑1 (MQ=255)
     ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCt                                                                                                                                       >  2:1708526/1‑150 (MQ=255)
     ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCt                                                                                                                                       <  1:473726/150‑1 (MQ=255)
     ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCt                                                                                                                                       <  2:730780/150‑1 (MQ=255)
         gCGTTGGCAAGGTGTGTCGTCGAAGCAACTACACACCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGa                                                                                                                                  <  1:1659276/150‑1 (MQ=255)
            ttGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAg                                                                                                                               >  2:548051/1‑150 (MQ=255)
            ttGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtggcgctg                                                                                                                                <  1:1335644/150‑8 (MQ=255)
                cAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtggcgct                                                                                                                                 >  1:412486/1‑139 (MQ=255)
                cAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtggcgct                                                                                                                                 <  2:412486/145‑7 (MQ=255)
                cAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTAAGGCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCg                                                                                                                           >  1:994551/1‑150 (MQ=255)
                 aaGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCgg                                                                                                                          <  1:532488/150‑1 (MQ=255)
                  aGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCgg                                                                                                                          >  2:1189206/1‑149 (MQ=255)
                  aGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCgg                                                                                                                          <  1:1189206/149‑1 (MQ=255)
                   ggTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTGTCGA‑AGGCCgcca                                                                                                                        <  1:936528/150‑4 (MQ=255)
                       tgtCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCggg                                                                                                                         <  1:737336/145‑1 (MQ=255)
                       tgtCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCggg                                                                                                                         >  2:737336/1‑145 (MQ=255)
                                aGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCgg                                                                                                           >  2:557948/1‑150 (MQ=255)
                                 gCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGcc                                                                                                                                        >  1:218304/1‑121 (MQ=255)
                                 gCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGcc                                                                                                                                        <  2:218304/121‑1 (MQ=255)
                                    aCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGActgg                                                                                                                  >  1:1144537/1‑139 (MQ=255)
                                    aCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGActgg                                                                                                                  <  2:1144537/139‑1 (MQ=255)
                                     cTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGacc                                                                                                      <  2:1697678/150‑1 (MQ=255)
                                        cAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGacccac                                                                                                   >  1:1281940/1‑150 (MQ=255)
                                         aaGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGacccacc                                                                                                  >  1:446715/1‑150 (MQ=255)
                                           gCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCg                                                                                                >  1:346352/1‑150 (MQ=255)
                                              gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                             <  1:282245/150‑1 (MQ=255)
                                              gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                             >  2:516805/1‑150 (MQ=255)
                                               aGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATgg                                                                                            <  2:1426021/150‑1 (MQ=255)
                                                 cAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTc                                                                                          <  1:557948/150‑1 (MQ=255)
                                                   aCCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                        <  2:446715/150‑1 (MQ=255)
                                                    ccGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTggcgg                                                                                                                                          >  1:1723268/1‑100 (MQ=255)
                                                    ccGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTggcgg                                                                                                                                          <  2:1723268/100‑1 (MQ=255)
                                                            gTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCAt                                                                               >  1:1540860/1‑150 (MQ=255)
                                                             ttGCCCGAATGGCCGCAGCAACGCGCCACCGCCGTGTATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATg                                                                              <  2:1384659/150‑1 (MQ=255)
                                                              tGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGc                                                                             <  2:1281940/150‑1 (MQ=255)
                                                                 ccGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGTGTGTTTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTgg                                                                          >  2:942839/1‑150 (MQ=255)
                                                                  cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                         >  1:1540403/1‑150 (MQ=255)
                                                                  cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCAT‑TCGAGAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                         >  1:195341/1‑150 (MQ=255)
                                                                    aaTGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTAc                                                                       <  2:346352/150‑1 (MQ=255)
                                                                          tGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTa                                                                        <  1:1014962/143‑1 (MQ=255)
                                                                          tGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTa                                                                        >  2:1014962/1‑143 (MQ=255)
                                                                             aGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCg                                                                                                            <  1:799747/104‑1 (MQ=255)
                                                                             aGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCg                                                                                                            >  2:799747/1‑104 (MQ=255)
                                                                                aTCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                           <  2:1929527/150‑1 (MQ=255)
                                                                                aTCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                           <  1:516805/150‑1 (MQ=255)
                                                                                aTCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                           >  2:1908158/1‑150 (MQ=255)
                                                                                 tCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgtg                                                          >  2:1746378/1‑150 (MQ=255)
                                                                                             cGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGc                                              <  2:1540860/150‑1 (MQ=255)
                                                                                                 cATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGc                                                                             >  2:272403/1‑115 (MQ=255)
                                                                                                 cATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGc                                                                             <  1:272403/115‑1 (MQ=255)
                                                                                                    ggtggtGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTAAAGGCCCTGGGGTGTCCGATGAGTGCCGGGCAg                                       <  1:1322692/150‑1 (MQ=255)
                                                                                                          gAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                             >  1:1627015/1‑122 (MQ=255)
                                                                                                          gAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                             <  2:1627015/122‑1 (MQ=255)
                                                                                                             tCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGAc                              >  1:310129/1‑150 (MQ=255)
                                                                                                               gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCg                                                                                                            <  1:746077/70‑1 (MQ=255)
                                                                                                               gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCg                                                                                                            >  2:746077/1‑70 (MQ=255)
                                                                                                               gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                            >  1:473686/1‑150 (MQ=255)
                                                                                                                ttCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATgcgc                                                                                    >  1:1457601/1‑93 (MQ=255)
                                                                                                                ttCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATgcgc                                                                                    <  2:1457601/93‑1 (MQ=255)
                                                                                                                    cTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGtgctgc                       >  2:1157817/1‑150 (MQ=255)
                                                                                                                     tGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGa                               <  1:62392/141‑1 (MQ=255)
                                                                                                                     tGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGa                               >  2:62392/1‑141 (MQ=255)
                                                                                                                       cgcTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGc                    <  2:473686/150‑1 (MQ=255)
                                                                                                                        gcTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCt                   >  2:546291/1‑150 (MQ=255)
                                                                                                                          tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                           >  1:1638638/1‑108 (MQ=255)
                                                                                                                          tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                           <  2:1638638/108‑1 (MQ=255)
                                                                                                                          tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGAGGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGt                 >  1:1634871/1‑150 (MQ=255)
                                                                                                                            tGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCa               <  1:548051/150‑1 (MQ=255)
                                                                                                                             gCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                            >  2:1888989/1‑136 (MQ=255)
                                                                                                                             gCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                            <  1:1888989/136‑1 (MQ=255)
                                                                                                                               cAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATgg                                                                                            <  2:364427/70‑1 (MQ=255)
                                                                                                                               cAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATgg                                                                                            >  1:364427/1‑70 (MQ=255)
                                                                                                                                     cAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCaa      >  2:620468/1‑150 (MQ=255)
                                                                                                                                     aaGGCCGCCAGCCAGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCaa      >  2:213634/2‑150 (MQ=255)
                                                                                                                                       ggCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAAtt    <  1:1708526/150‑1 (MQ=255)
                                                                                                                                       ggCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAAtt    <  2:1904510/150‑1 (MQ=255)
                                                                                                                                        gccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTg   >  1:296596/1‑150 (MQ=255)
                                                                                                                                         ccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGc                                      >  2:280779/1‑114 (MQ=255)
                                                                                                                                         ccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGc                                      <  1:280779/114‑1 (MQ=255)
                                                                                                                                         ccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTgg  >  1:1091321/1‑149 (MQ=255)
                                                                                                                                         ccgccTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATCCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGa  <  1:620468/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCT‑TCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGA  >  NC_002947/2560363‑2560649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑