Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,645,153 T→C 5.2% E735G (GAG→GGG)  PP_2316 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,645,1530TC 5.2% 228.5 / 2.2 78E735G (GAG→GGG) PP_2316ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (36/37);  new base C (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

CAGCACCCAGGTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAGGCATCCACCTGCAGGATGGTCACCG  >  NC_002947/2645008‑2645300
                                                                                                                                                  |                                                                                                                                                    
cAGCACCCAGGTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGTTGGCCGCACCCAGCAGACCGAACTCGATTCGCCGGGCCTTGACCAGCACTACGCGGCCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGGGCTCATc                                                                                                                                                  >  1:1574231/1‑150 (MQ=255)
  gCACCCAGTTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCa                                                                                                                                                <  2:1458539/150‑1 (MQ=255)
    aCCCAGGTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGc                                                                                                                                              >  1:1674260/1‑150 (MQ=255)
      ccAGGTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGt                                                                                                                                            >  1:637703/1‑150 (MQ=255)
      ccAGGTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGt                                                                                                                                            >  1:1571065/1‑150 (MQ=255)
      ccAGGTAATCAGTTCGCAGCCCACGGCCCCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCCGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGt                                                                                                                                            >  1:261035/1‑150 (MQ=255)
          gTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGcc                                                                                                                                        >  1:1172508/1‑150 (MQ=255)
          gTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGACCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGcc                                                                                                                                        <  1:483202/150‑1 (MQ=255)
              tCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGTCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCa                                                                                                                                    >  2:1355948/1‑150 (MQ=255)
               cAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCaa                                                                                                                                   >  1:1599384/1‑150 (MQ=255)
                    cGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCgg                                                                                                                              <  1:598145/150‑1 (MQ=255)
                        gCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTACGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGaa                                                                                                                          <  1:1876859/150‑1 (MQ=255)
                           cACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCTCGCAACAGTCCCCCTTGGAGACTGCGCTCATCCAGCGTCGCCTGCAAGCCGGCGAACAg                                                                                                                       <  1:536131/150‑1 (MQ=255)
                            aCGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGc                                                                                                                      >  1:601744/1‑150 (MQ=255)
                                 cgccAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGc                                                                                                                 <  2:1571065/150‑1 (MQ=255)
                                 caacAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGc                                                                                                                 <  2:268370/147‑1 (MQ=255)
                                  gctaagaGCCCGCTGGCCCTACCCGAGAGGCCGAACGCGATTCGCCGTGCCTTAACCAGCAACGGCCGCGCCG‑CGCCCACTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGcc                                                                                                                <  2:734328/144‑1 (MQ=255)
                                    cAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCTAACAGCACTGCCAg                                                                                                              <  1:755884/150‑1 (MQ=255)
                                          cccGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCcggc                                                                                                        >  1:136386/1‑150 (MQ=255)
                                               tGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑Ca                                                                                                  <  2:1172508/150‑1 (MQ=255)
                                                  ccGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCa                                                                                               <  2:961697/150‑1 (MQ=255)
                                                   cGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCAc                                                                                              <  2:756342/150‑1 (MQ=255)
                                                   cGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCAc                                                                                              >  1:1310942/1‑150 (MQ=255)
                                                      aCCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGaa                                                                                           >  1:1648707/1‑150 (MQ=255)
                                                      aCCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGaa                                                                                           >  2:869080/1‑150 (MQ=255)
                                                        ccAGCAGGCCGAACTCTATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATCCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACa                                                                                         >  1:1269802/1‑150 (MQ=255)
                                                        ccAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACa                                                                                         >  2:889213/1‑150 (MQ=255)
                                                            cagGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCaa                                                                                     <  1:889213/150‑1 (MQ=255)
                                                             agGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAac                                                                                    <  2:261035/150‑1 (MQ=255)
                                                             agGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAac                                                                                    >  2:1893629/1‑150 (MQ=255)
                                                              ggCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAaca                                                                                   <  2:914423/150‑1 (MQ=255)
                                                               gCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGc                                                                                                                             <  1:1918290/108‑1 (MQ=255)
                                                               gCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGc                                                                                                                             >  2:1918290/1‑108 (MQ=255)
                                                               gCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGCCCGCACCGACACCCA‑CGCGCACACTCGTGCCCCTTGGCGAGTTCTTTCATCCAGCGGGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAacac                                                                                  <  2:1674260/150‑1 (MQ=255)
                                                                ccGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAAcaca                                                                                 <  2:42687/150‑1 (MQ=255)
                                                                ccGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAAcaca                                                                                 >  1:1093107/1‑150 (MQ=255)
                                                                ccGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAAcaca                                                                                 <  1:38371/150‑1 (MQ=255)
                                                                      tCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTcc                                                                           >  1:827433/1‑150 (MQ=255)
                                                                      tCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTcc                                                                           >  2:1101022/1‑150 (MQ=255)
                                                                        gATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCAc                                                                         >  2:1759140/1‑150 (MQ=255)
                                                                         aTTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACt                                                                                                                   <  1:1223093/108‑1 (MQ=255)
                                                                         aTTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACt                                                                                                                   >  2:1223093/1‑108 (MQ=255)
                                                                          ttCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCg                                                                       <  2:136386/150‑1 (MQ=255)
                                                                              ccGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAg                                                                   <  1:1101022/150‑1 (MQ=255)
                                                                               cGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAgg                                                                  <  2:1679692/150‑1 (MQ=255)
                                                                                    cTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTAc                                                             <  1:1820641/150‑1 (MQ=255)
                                                                                        aCCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTg                                                                                                                                           <  1:59786/69‑1 (MQ=255)
                                                                                        aCCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTg                                                                                                                                           >  2:59786/1‑69 (MQ=255)
                                                                                           aGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGcgcg                                                      >  1:495841/1‑150 (MQ=255)
                                                                                            gCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTTCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCga                                                     >  1:941556/1‑150 (MQ=255)
                                                                                            gCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGt                                                                <  1:875686/139‑1 (MQ=255)
                                                                                            gCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGt                                                                >  2:875686/1‑139 (MQ=255)
                                                                                               aCGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGaa                                                  <  2:290268/150‑1 (MQ=255)
                                                                                               aCGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGaa                                                  >  1:1025058/1‑150 (MQ=255)
                                                                                                 ggCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAAtg                                                <  2:601744/150‑1 (MQ=255)
                                                                                                  gccgcgccg‑cGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAAtgc                                               >  1:432214/1‑150 (MQ=255)
                                                                                                  cccgcgccg‑cgCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAAtgt                                               >  1:1827540/2‑149 (MQ=255)
                                                                                                         cg‑cgccCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGGACCGACATGAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAg                                        >  2:1021538/1‑150 (MQ=255)
                                                                                                          g‑cgccCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGc                                       >  1:1786474/1‑150 (MQ=255)
                                                                                                            cgccCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCt                                      <  1:1355948/150‑1 (MQ=255)
                                                                                                               ccAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCCCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCcggcg                                                                                                       >  1:161629/1‑83 (MQ=255)
                                                                                                               ccAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCCCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCcggcg                                                                                                       <  2:161779/83‑1 (MQ=255)
                                                                                                               ccAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCCCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCcggcg                                                                                                       <  2:161629/83‑1 (MQ=255)
                                                                                                               ccAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCCCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCcggcg                                                                                                       >  1:161779/1‑83 (MQ=255)
                                                                                                               caaGTGCGCGCAACAGTGCTCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGt                                   >  1:630371/3‑150 (MQ=255)
                                                                                                                cAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTc                                  <  2:1599384/150‑1 (MQ=255)
                                                                                                                      gcgcAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCaa                            <  2:637703/150‑1 (MQ=255)
                                                                                                                       cgcAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAg                           <  2:1648707/150‑1 (MQ=255)
                                                                                                                       cgcAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAg                           <  2:1025058/150‑1 (MQ=255)
                                                                                                                        gcAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAgg                          >  2:1860884/1‑150 (MQ=255)
                                                                                                                            cAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAGGCATc                      <  2:1093107/150‑1 (MQ=255)
                                                                                                                                   ccTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTAGTCGAGCAAGGCATCCACCTGc               >  2:1699810/1‑150 (MQ=255)
                                                                                                                                    cTTGGCGCATGCGCGGAGCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGACAGCAGGGGTGCGTAGC‑GGATGGCGAACACATACTCCGCCGACAGGGTTACCTGCGCGGGCCAGCTGCGCAGTTGGTCGAGCAAGGCATCCACCTGCa              <  1:1893629/150‑1 (MQ=255)
                                                                                                                                    cTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAGGCATCCACCTGCa              <  2:1310942/150‑1 (MQ=255)
                                                                                                                                     ttGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAATAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAGGCATCCACCTGCAg             <  2:1695912/150‑1 (MQ=255)
                                                                                                                                                gcTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTa                                                              >  1:998625/1‑90 (MQ=255)
                                                                                                                                                gcTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTa                                                              <  2:998625/90‑1 (MQ=255)
                                                                                                                                                gcTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAGGCATCCACCTGCAGGATGGTCAccg  >  2:382449/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CAGCACCCAGGTAATCAGTTCGCAGCCCACGGCCGCCAATAGCCCGCTGGCCGCACCCAGCAGGCCGAACTCGATTCGCCGGGCCTTGACCAGCAACGGCCGCGCCG‑CGCCCAGTGCGCGCAACAGTGCCCCTTGGCGAATGCGCTCATCCAGCGTGGCCTGCAAGCCGGCGAACAGCACTGCCAGCCCGGCGGC‑CAGCACGAACAGCAACACATACTCCACCGCCAGGGTTACCTGCGCGAGAATGCTGCGCAGCTGGTCGAGCAAGGCATCCACCTGCAGGATGGTCACCG  >  NC_002947/2645008‑2645300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑