Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,203 A→T 7.1% intergenic (+99/+35) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,2030AT 7.1% 164.5 / 4.6 57intergenic (+99/+35)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base T (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

ACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTCG  >  NC_002947/2650055‑2650352
                                                                                                                                                    |                                                                                                                                                     
aCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACaa                                                                                                                                                      <  2:1935336/150‑1 (MQ=255)
 ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAg                                                                                                                                                     >  1:444149/1‑150 (MQ=255)
 ccAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTGTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAATGCACTACCAAAGTCGATCGACAAg                                                                                                                                                     >  2:244366/1‑150 (MQ=255)
   aGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTg                                                                                                                                                   >  2:1843726/1‑150 (MQ=255)
           gCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTGCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGct                                                                                                                                           >  1:261679/1‑150 (MQ=255)
             aCCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAATAAATCCGACCCACAAGTGGGTCGGcttc                                                                                                                                         <  1:244366/150‑1 (MQ=255)
                           ggaTTGCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCGCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                                                                                           <  2:405319/148‑1 (MQ=255)
                           gCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAATGCACTAAACCAGCCGTCCCTCCAGTGGGTCGGCTTCTTAACAGTCCGTGa                                                                                                                           <  2:805442/150‑1 (MQ=255)
                           gCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                                                                                           <  1:706634/150‑1 (MQ=255)
                                   cccGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGattatt                                                                                                                   >  2:1856938/1‑150 (MQ=255)
                                          tGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCg                                                                                                            >  1:1758209/1‑150 (MQ=255)
                                            cAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGct                                                                                                                                           <  1:166373/117‑1 (MQ=255)
                                            cAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACTTGTGGGTCGGct                                                                                                                                           >  2:166373/1‑117 (MQ=255)
                                             aGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggct                                                                                                         >  2:602602/1‑150 (MQ=255)
                                                 aGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggct                                                                                                     <  1:602602/150‑1 (MQ=255)
                                                     aaTTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGt                                                                                                 >  2:1631223/1‑150 (MQ=255)
                                                        ttCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCa                                                                                              <  2:1758209/150‑1 (MQ=255)
                                                                 cccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCa                                                                                     <  2:444149/150‑1 (MQ=255)
                                                                  ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTaa                                                                                                                                     >  2:785243/1‑101 (MQ=255)
                                                                  ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTaa                                                                                                                                     <  1:785243/101‑1 (MQ=255)
                                                                  ccAGACCTGCCCTTAGTGCAGGCCTTTTCCTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGACTGGCTTTGTCCAGCATACGCAg                                                                                    >  1:1809072/1‑150 (MQ=255)
                                                                        cTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAg                                                                                                                          >  2:816139/1‑106 (MQ=255)
                                                                        cTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAg                                                                                                                          <  1:816139/106‑1 (MQ=255)
                                                                          gCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACtt                                                                                                               <  2:402877/115‑1 (MQ=255)
                                                                          gCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACtt                                                                                                               >  1:402877/1‑115 (MQ=255)
                                                                           cccTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTc                                                                           <  1:1764118/150‑1 (MQ=255)
                                                                             cTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGt                                                                         >  1:1030623/1‑150 (MQ=255)
                                                                               tAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTg                                                                       <  1:1883153/150‑1 (MQ=255)
                                                                                 gTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGc                                                                     <  1:111671/150‑1 (MQ=255)
                                                                                    cAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGGGAGGATTATTACTTGCGACAGCCTTTGTCTAGCATACGCAGGGCGCGCTCGTTGGCTTc                                                                  >  2:458996/1‑150 (MQ=255)
                                                                                         cTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAg                                                             <  2:1030623/150‑1 (MQ=255)
                                                                                          ttttCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTg                                                                       >  2:8961/1‑139 (MQ=255)
                                                                                          ttttCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTg                                                                       <  1:8961/139‑1 (MQ=255)
                                                                                          ttttCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGc                                                            >  2:1708319/1‑150 (MQ=255)
                                                                                           tttCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCg                                                           <  1:200306/150‑1 (MQ=255)
                                                                                               gTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGACTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTct                                                       <  2:1809072/150‑1 (MQ=255)
                                                                                                  ccTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGGTGGCTTCGTCAGCGGTctgct                                                    <  1:1843726/150‑1 (MQ=255)
                                                                                                    tgctgACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCttt                                                                                                   >  2:119658/1‑101 (MQ=255)
                                                                                                    tgctgACCGCGTTTGCCGGTAACAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCttt                                                                                                   <  1:119658/101‑1 (MQ=255)
                                                                                                           cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACGCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGtt                                                                        >  1:1568379/1‑121 (MQ=255)
                                                                                                           cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGc                                                                                      >  1:1921396/1‑107 (MQ=255)
                                                                                                           cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGc                                                                                      <  2:1921396/107‑1 (MQ=255)
                                                                                                           cgcgTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGtt                                                                        <  2:1568379/121‑1 (MQ=255)
                                                                                                                 tGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCgg                                     <  1:1856938/150‑1 (MQ=255)
                                                                                                                    cGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCtt                                             >  2:538707/1‑139 (MQ=255)
                                                                                                                    cGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCtt                                             <  1:538707/139‑1 (MQ=255)
                                                                                                                         tCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGTCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCAtt                             <  2:650071/150‑1 (MQ=255)
                                                                                                                           aaaGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggc                                                                                                      >  2:191191/1‑75 (MQ=255)
                                                                                                                           aaaGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggc                                                                                                      <  1:191191/75‑1 (MQ=255)
                                                                                                                              ggCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctgg                                                                                                       <  1:1575599/71‑1 (MQ=255)
                                                                                                                              ggCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctgg                                                                                                       >  2:1575599/1‑71 (MQ=255)
                                                                                                                               gCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGcgtcg                       >  2:769859/1‑150 (MQ=255)
                                                                                                                                       gaaGCCGACCCACTTGTGGGTCGGCTTTTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAg                                                                                             <  1:1922243/71‑1 (MQ=25)
                                                                                                                                       gaaGCCGACCCACTTGTGGGTCGGCTTTTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAg                                                                                             >  2:1922243/2‑72 (MQ=25)
                                                                                                                                         aGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTAc             <  1:1631223/150‑1 (MQ=255)
                                                                                                                                          gCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACg            >  1:897076/1‑150 (MQ=255)
                                                                                                                                                ccACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTa              <  1:350864/142‑1 (MQ=255)
                                                                                                                                                ccACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTa              >  2:350864/1‑142 (MQ=255)
                                                                                                                                                  acaAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCtt    >  1:95683/1‑150 (MQ=255)
                                                                                                                                                    aaGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTCg  >  2:618119/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGTTGGCTTCGTCAGCGGTCTGCTGAGCCTTCTGAGCGGCTGCCATTGCGTCGTCAGCCTTACGGTAGGCTTCG  >  NC_002947/2650055‑2650352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑