| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,788,121 | T→A | 8.0% | V134D (GTC→GAC) | ahpF → | alkyl hydroperoxide reductase subunit F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,788,121 | 0 | T | A | 8.0% | 142.3 / 5.5 | 50 | V134D (GTC→GAC) | ahpF | alkyl hydroperoxide reductase subunit F |
| Reads supporting (aligned to +/- strand): ref base T (25/21); new base A (2/2); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01 | |||||||||||
GAGTTCACATCGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGTCAGGGC > NC_002947/2787973‑2788269 | gAGTTCACATCGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGAcgtc < 1:525288/150‑1 (MQ=255) cGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCAc < 1:1200815/150‑1 (MQ=255) cGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCAc < 1:30404/150‑1 (MQ=255) ggtggCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAAcccc > 1:999721/1‑150 (MQ=255) ccACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGAGCCAGGCCCTGGAAGGTGAATTCAACTTCTGATGCTCCTTCACCCGTACGGGCCAGGACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTGCGGAACCCCAACGt > 1:629721/1‑150 (MQ=255) aCCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTgc < 2:1525963/150‑1 (MQ=255) ccGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTgcgc > 1:1649067/1‑150 (MQ=255) cGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACgaaaggc < 1:906234/150‑7 (MQ=255) aaGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCAAGACACTGATCCGGATGGCGGGAGGCAACCCCAACGTGCGCCATGtt > 2:1287499/1‑150 (MQ=255) gCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGcc > 1:1363882/1‑150 (MQ=255) cGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCTGGACGACGTCCGGGCagtt > 2:421649/1‑97 (MQ=255) cGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCTGGACGACGTCCGGGCagtt < 1:421649/100‑4 (MQ=255) gAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGt < 2:1146084/150‑1 (MQ=255) aTCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTg > 2:135282/1‑150 (MQ=255) aTCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTg > 1:1500798/1‑150 (MQ=255) cGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGtt < 2:1500798/150‑1 (MQ=255) gAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAAc > 1:468485/1‑111 (MQ=255) gAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAAc < 2:468485/111‑1 (MQ=255) cAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCAAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGaa > 2:276263/1‑150 (MQ=255) cccTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTgg > 1:1929041/1‑150 (MQ=255) cTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGaa < 2:1363882/150‑1 (MQ=255) cTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGaa > 2:606715/1‑150 (MQ=255) ggTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTAGTACAGGCACTGAACCTGGTGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCgcgc > 1:725490/1‑150 (MQ=255) aaTTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGa > 2:1292222/1‑150 (MQ=255) tCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGa < 2:531111/69‑1 (MQ=255) tCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGa > 1:531111/1‑69 (MQ=255) tCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCa < 1:606715/150‑1 (MQ=255) aaCTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATg > 2:276104/1‑150 (MQ=255) aCTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATgg < 2:1742466/150‑1 (MQ=255) gAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGCCGAAGGATATTCGCGCAAGATCATGGCGGTa > 2:933465/1‑150 (MQ=255) cTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTAt < 1:759834/150‑1 (MQ=255) ttCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATc > 2:1758906/1‑150 (MQ=255) ctcGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTa > 2:304262/1‑150 (MQ=255) ctcGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTa < 2:629721/150‑1 (MQ=255) tcGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTAc < 1:276104/150‑1 (MQ=255) tcGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTAc < 1:1292222/150‑1 (MQ=255) tcGCTGGCGGGCCAGAACTTCCCGGACGTCCTCCAGGCACTGAACCTGATGGCGGTACTCAACACCAACGTGCGCCATGTTGCCACAGACGGTCCGCTGTTCAACGATGATTTGGAATCGCGCAAGACCATGGCGGTACCGCGTATCTAc < 2:559386/150‑1 (MQ=255) ctgatCGTGCCAGAACTGCCCCGACGTCGTCCAGGCACTGACCCTGATGGCGGCACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTGCAGGACGAAGTGGACTCGCGCAAGATCGTGGAGGTACCGAGTATCTAcca > 1:559386/5‑149 (MQ=255) gTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCaa < 1:1287499/150‑1 (MQ=255) tCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCGGGACGACGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAAc > 1:1564947/1‑150 (MQ=255) gTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCt > 2:1429948/1‑90 (MQ=255) gTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCt < 1:1429948/90‑1 (MQ=255) gTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACgg > 2:1002634/1‑150 (MQ=255) gCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGc > 2:499332/1‑124 (MQ=255) gCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGc < 1:499332/124‑1 (MQ=255) aGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAgg > 2:1932604/1‑150 (MQ=255) aaCTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGgtg > 2:1454131/1‑150 (MQ=255) tGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGttt < 1:500763/150‑1 (MQ=255) gcctggACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGa < 1:1764203/119‑1 (MQ=255) gCCTGGACGACGTCCGGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGa > 2:1764203/1‑123 (MQ=255) gACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGTCAg < 2:1649067/150‑1 (MQ=255) gtcgtcCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGTCAGGGc > 1:986346/1‑150 (MQ=255) | GAGTTCACATCGTTGGTGCTGGCACTGCTGCAAGTGGGTGGCCACCCGTCAAAGGCCAGTGCCGAAGTGATCGAGCAGATCCAGGCCCTGGAAGGTGAATTCAACTTCGAAACCTACTTCTCGCTGTCGTGCCAGAACTGCCCGGACGTCGTCCAGGCACTGAACCTGATGGCGGTACTCAACCCCAACGTGCGCCATGTTGCCATCGACGGTGCGCTGTTCCAGGACGAAGTGGAATCGCGCAAGATCATGGCGGTACCGAGTATCTACCTCAACGGTGAGGTGTTTGGTCAGGGC > NC_002947/2787973‑2788269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |