Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,006,511 T→G 9.4% F1017V (TTC→GTC)  PP_2627 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,006,5110TG 9.4% 166.5 / 11.2 64F1017V (TTC→GTC) PP_2627membrane protein
Reads supporting (aligned to +/- strand):  ref base T (31/27);  new base G (4/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

GACGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCA  >  NC_002947/3006367‑3006655
                                                                                                                                                |                                                                                                                                                
gACGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTg                                                                                                                                             >  2:1897264/1‑150 (MQ=255)
 aCGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGa                                                                                                                                            <  2:1623946/150‑1 (MQ=255)
      tACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGAc                                                                                                                                       >  2:1056472/1‑150 (MQ=255)
           ttttttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGAGGCTTGTTGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCt                                                                                                                                  >  1:120762/1‑150 (MQ=255)
            tttttCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTTTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCtt                                                                                                                                 <  2:53259/150‑1 (MQ=255)
                       aCGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCa                                                                                                                      >  1:224058/1‑150 (MQ=255)
                           cTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCATCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTcc                                                                                                                  >  2:1246933/1‑150 (MQ=255)
                           cTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTcc                                                                                                                  >  2:1437381/1‑150 (MQ=255)
                                aGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCa                                                                                                             <  2:465580/150‑1 (MQ=255)
                                       tACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACggg                                                                                                      >  2:332314/1‑150 (MQ=255)
                                         ccccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCa                                                                                                    >  1:1913475/1‑150 (MQ=255)
                                          cccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCaa                                                                                                   >  1:1489462/1‑150 (MQ=255)
                                           ccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAc                                                                                                                                    >  2:191410/1‑116 (MQ=255)
                                           ccTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAc                                                                                                                                    <  1:191410/116‑1 (MQ=255)
                                              ttGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATccc                                                                                               <  1:1437381/150‑1 (MQ=255)
                                                 tggtgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                            >  1:311894/1‑150 (MQ=255)
                                                  ggtgAAGGCACCCGATGCTTCTTTGCATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGAt                                                                                           <  1:208229/150‑1 (MQ=255)
                                                    tgAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTc                                                                                         >  1:1332374/1‑150 (MQ=255)
                                                        ggCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGc                                                                                     >  2:622689/1‑150 (MQ=255)
                                                         gCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGTGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGcc                                                                                    >  2:124825/1‑150 (MQ=255)
                                                            cccGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCATGGGCAATCCCTGATTCGTGCCGAc                                                                                 <  1:1897264/150‑1 (MQ=255)
                                                                   cttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCg                                                                          <  2:224058/150‑1 (MQ=255)
                                                                    ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCt                                                                                                                                  >  1:558653/1‑93 (MQ=38)
                                                                    ttcttTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTGTCCTTCAGGAAAACCt                                                                                                                                  <  2:558653/93‑1 (MQ=38)
                                                                        ttGGATGACTTCGAGGCGTTCGTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGagcag                                                                     <  1:1769505/150‑1 (MQ=255)
                                                                          ggATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCt                                                                   >  1:225212/1‑150 (MQ=255)
                                                                               aCTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                            <  1:1855615/120‑1 (MQ=255)
                                                                               aCTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                            >  2:1855615/1‑120 (MQ=255)
                                                                               aCTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGc                                                              <  2:1670183/150‑1 (MQ=255)
                                                                                 ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAc                                                                                                                                    >  1:1343321/1‑78 (MQ=255)
                                                                                 ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAAc                                                                                                                                    <  2:1343321/78‑1 (MQ=255)
                                                                                 ttCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCAGGCTGGCCCGGCTGTTGCGTGATTGTTGCCGACGTGATCGAGCAGCTCGAGCTg                                                            >  2:542630/1‑150 (MQ=255)
                                                                                  tCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTgg                                                           <  1:1056472/150‑1 (MQ=255)
                                                                                    gAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                            <  2:604364/115‑1 (MQ=255)
                                                                                    gAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGa                                                                                            >  1:604364/1‑115 (MQ=255)
                                                                                    gAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCg                                                         <  1:622689/150‑1 (MQ=255)
                                                                                     aGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAg                                                                                                                                          <  2:842186/68‑1 (MQ=255)
                                                                                     aGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAg                                                                                                                                          >  1:842186/1‑68 (MQ=255)
                                                                                       gCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTC‑AGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                     <  2:1114939/150‑1 (MQ=255)
                                                                                        cGTTCTTCGGGCCGAAAGGGCCCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                     <  1:297865/150‑1 (MQ=255)
                                                                                                gggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgc                                             >  1:473884/1‑150 (MQ=255)
                                                                                                 ggCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCcgcg                                            >  1:1225355/1‑150 (MQ=255)
                                                                                                    cGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGaaa                                         >  1:151006/1‑150 (MQ=255)
                                                                                                     gAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCACCCGCGAAAc                                        <  1:797148/150‑1 (MQ=255)
                                                                                                               cTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGAt                              <  1:1498680/150‑1 (MQ=255)
                                                                                                                  cagcagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCg                                                                 <  2:1781408/112‑1 (MQ=255)
                                                                                                                  cagcagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCg                                                                 >  1:1781408/1‑112 (MQ=255)
                                                                                                                  cagcagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAg                           >  2:263430/1‑150 (MQ=255)
                                                                                                                   agcagTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGc                          >  1:205839/1‑150 (MQ=255)
                                                                                                                      agTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTg                       <  2:151006/150‑1 (MQ=255)
                                                                                                                          tAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCaa                   >  2:1101101/1‑150 (MQ=255)
                                                                                                                          tAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCaa                   <  2:473884/150‑1 (MQ=255)
                                                                                                                          tAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCaa                   <  1:1738223/150‑1 (MQ=255)
                                                                                                                                tCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAg             >  1:716077/1‑150 (MQ=255)
                                                                                                                                    tATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGtgtg         <  1:1246933/150‑1 (MQ=255)
                                                                                                                                     gcctcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGc        >  2:1236722/3‑150 (MQ=255)
                                                                                                                                        tcAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCTGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt     >  1:121002/1‑150 (MQ=255)
                                                                                                                                        tcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                     >  1:950549/1‑102 (MQ=38)
                                                                                                                                        tcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAgc                                                     <  2:950549/102‑1 (MQ=38)
                                                                                                                                        tcAAGGTTGTCCTTCAGGAAAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGt     >  1:689352/1‑150 (MQ=255)
                                                                                                                                         cAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCa                            >  2:966394/1‑126 (MQ=255)
                                                                                                                                         cAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCa                            <  1:966394/126‑1 (MQ=255)
                                                                                                                                          aaGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTc   >  2:111519/1‑150 (MQ=255)
                                                                                                                                           aGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCa  <  1:263430/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GACGTGTACAGTTTTTTCCAGCAACGCCTGGCAGGCCGCTACCCCTTTGTGGTGAAGGCACCCGATGCTTCTTTGGATGACTTCGAGGCGTTCTTCGGGCCGAAAGGGCGCCTGCAGCAGTTTAATGATCGGTATCTCAAGGTTTTCCTGAAGGACAACCTTGAGGCCTTGCAGTCCGCTCAGCACGGGCAATCCCTGATTCGTGCCGACGTGATCGAGCAGCTCGAGCTGGCGGAGCGCATCCGCGAAACCTTCTTCGATCAGCGTGGCAAGCTAAGTGTGCAGTTCA  >  NC_002947/3006367‑3006655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑