Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,011,012	A→C	5.0%	V10V (GTA→GTC)	bcsG →	endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,011,012	0	A	C	5.0%	243.0 / 4.4	80	V10V (GTA→GTC)	bcsG	endoglucanase
Reads supporting (aligned to +/- strand):  ref base A (37/39);  new base C (2/2);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

GCCTTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCT  >  NC_002947/3010865‑3011158                                                                                                                                                    |                                                                                                                                                   gcctTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACt                                                                                                                                                  >  1:33162/1‑150 (MQ=255)    ttgttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTgg                                                                                                                                                <  1:851303/149‑1 (MQ=255)    ttgttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTgg                                                                                                                                                >  2:851303/1‑149 (MQ=255)       ttgCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCg                                                                                                                                            >  1:861871/1‑150 (MQ=255)         gCTAGTGCGCCTGTGTTCGGGATTACGTGGCGTGGTGCGCCGTCGCCTTCCCCCCCGTTACCAGAAACCGCTGGGCGTACGCGGCCGCGCTGCGCGCGAAGGACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGACCCGCt                                                                                                                                          >  2:545136/1‑150 (MQ=255)           tAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTgg                                                                                                                                        >  2:598857/1‑150 (MQ=255)           tAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTgg                                                                                                                                        >  2:227908/1‑150 (MQ=255)                 gcCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGa                                                                                                                                  >  2:894761/1‑150 (MQ=255)                   cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTa                                                                                                                                                    >  1:796310/1‑130 (MQ=255)                   cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTa                                                                                                                                                    <  2:796310/130‑1 (MQ=255)                   cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTg                                                                                                                                    <  2:1086842/146‑1 (MQ=255)                   cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTg                                                                                                                                    >  1:1086842/1‑146 (MQ=255)                   cTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACt                                                                                                                                >  1:1801983/1‑150 (MQ=255)                    tgtgTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTg                                                                                                                               >  1:1561929/1‑150 (MQ=255)                     gtgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                              <  2:30288/150‑1 (MQ=255)                      tgtTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTggg                                                                                                                             >  2:1937351/1‑150 (MQ=255)                          cGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTCGCCTGGACTGGGAggg                                                                                                                         <  1:1042009/150‑1 (MQ=255)                           ggCATTACGTGGCGTGTTGCGCCGTCGCGTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCCCCCCCCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCCCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGt                                                                                                                        <  1:120249/150‑1 (MQ=255)                              aTTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCAGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGa                                                                                                                     >  1:873225/1‑150 (MQ=255)                                tACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAc                                                                                                                   >  2:1461827/1‑150 (MQ=255)                                 aCGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAAcc                                                                                                                  <  1:1911882/150‑1 (MQ=255)                                    tGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                               >  2:1378024/1‑150 (MQ=255)                                            tGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                       <  2:1801983/150‑1 (MQ=255)                                             gcgcCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTg                                                                                                      <  2:861871/150‑1 (MQ=255)                                                 cGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCt                                                                                                                                          <  1:1260261/110‑1 (MQ=255)                                                 cGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCt                                                                                                                                          >  2:1260261/1‑110 (MQ=255)                                                 cGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaaa                                                                                                  >  2:1459902/1‑150 (MQ=255)                                                 cGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCaaa                                                                                                  <  1:1459902/150‑1 (MQ=255)                                                     gCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGaa                                                                                                                    >  2:1519312/1‑128 (MQ=255)                                                     gCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGaa                                                                                                                    <  1:1519312/128‑1 (MQ=255)                                                     gCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGACCAGCTCCAAAGGAGGAACCCGAGACTGCTTCATGCCGCGAACAGCCCCGCTGTACTTGCCCGCGTGCCGTGGCTGGGGGGGTGGAACCTCTATTTTTTTGCAAAGttt                                                                                              >  2:1189426/1‑150 (MQ=255)                                                      ccTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                              >  2:1235375/1‑117 (MQ=255)                                                      ccTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTgg                                                                                                                              <  1:1235375/117‑1 (MQ=255)                                                        ttCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                          >  2:469936/1‑119 (MQ=255)                                                        ttCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAgg                                                                                                                          <  1:469936/119‑1 (MQ=255)                                                          cccccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCACCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTgatgg                                                                                                                   >  1:1188372/1‑120 (MQ=18)                                                          cccccccGTTACCTGAAACCGATGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCACCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTgatgg                                                                                                                   <  2:1188372/124‑5 (MQ=18)                                                           ccccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTctgctg                                                                                        >  2:702399/1‑150 (MQ=255)                                                            cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                               <  2:787619/126‑1 (MQ=255)                                                            cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACctct                                                                                                               >  1:787619/1‑126 (MQ=255)                                                            cccccGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTgg                                                                                       <  2:926077/150‑1 (MQ=255)                                                                cGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTcgc                                                                                   <  1:702399/150‑1 (MQ=255)                                                                     ccTGAAACCGCCGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACAGCGGCGCACTCTGTACTGGCCCGCTGGCCTGGACCGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTgg                                                                              <  1:1304832/150‑1 (MQ=255)                                                                      cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                             >  1:577301/1‑150 (MQ=255)                                                                      cTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTggg                                                                             >  1:573694/1‑150 (MQ=255)                                                                       tGAAACCGCTGGGCTTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGc                                                                            >  1:251976/1‑150 (MQ=255)                                                                             cGCTGGGCGTCCGCTGCCGCGCTGCACCCCAGGAACCCCACAATGACTAATGCCCCCATCAGCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTc                                                                      <  2:577301/150‑1 (MQ=255)                                                                             cGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTc                                                                      <  1:910304/150‑1 (MQ=255)                                                                              gCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCg                                                                     >  2:1563081/1‑150 (MQ=255)                                                                               cTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAANCTCTATNTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGa                                                                    <  1:1356143/150‑1 (MQ=255)                                                                                    cGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTcc                                                               <  1:412646/150‑1 (MQ=255)                                                                                     gTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttt                                                                                                           >  1:951699/1‑105 (MQ=255)                                                                                     gTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttt                                                                                                           <  2:951699/105‑1 (MQ=255)                                                                                      tACGCCGCCGCGCTGCACCCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAg                                                             <  1:1042391/150‑1 (MQ=255)                                                                                            gccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTg                                                       <  2:91903/150‑1 (MQ=255)                                                                                             ccgcGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCCCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGc                                                      <  1:750151/150‑1 (MQ=255)                                                                                               gcgcTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTgat                                                                                                                     >  1:1924025/1‑83 (MQ=9)                                                                                               gcgcTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCAGTCCAGGCCAGCGGGCCAGTACCGGGAGGGTgat                                                                                                                     <  2:1924025/85‑3 (MQ=9)                                                                                                     cACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtg                                              <  2:1561929/150‑1 (MQ=255)                                                                                                     cACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGACATGCTc                                                                      >  1:1655024/1‑126 (MQ=255)                                                                                                     cACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGACATGCTc                                                                      <  2:1655024/126‑1 (MQ=255)                                                                                                       cGCCAGGAACCCCAGAATGACTAATACCACCATCCCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCCCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCtgtt                                            <  1:1236981/150‑1 (MQ=255)                                                                                                        gCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                       >  1:1468876/1‑90 (MQ=255)                                                                                                        gCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTAttttttt                                                                                                       <  2:1468876/90‑1 (MQ=255)                                                                                                             gAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGc                                      <  2:251976/150‑1 (MQ=255)                                                                                                               aCCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGTTg                                    <  1:1937351/150‑1 (MQ=255)                                                                                                                  ccAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGt                                 >  2:549260/1‑150 (MQ=255)                                                                                                                   cAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGtt                                >  2:795296/1‑150 (MQ=255)                                                                                                                          aCTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCtt                         <  1:1378024/150‑1 (MQ=255)                                                                                                                            tAATACCACCATCTCCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGt                       <  1:977071/150‑1 (MQ=255)                                                                                                                              aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                           <  2:985551/144‑1 (MQ=255)                                                                                                                              aTACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGc                           >  1:985551/1‑144 (MQ=255)                                                                                                                                 ccaccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCtt                  >  2:1111540/1‑150 (MQ=255)                                                                                                                                  caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCttt                 <  2:422668/150‑1 (MQ=255)                                                                                                                                  caccaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCttt                 >  1:422668/1‑150 (MQ=255)                                                                                                                                   accaTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTg                >  2:806942/1‑150 (MQ=255)                                                                                                                                      aTCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCATAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCg             >  2:1129408/1‑150 (MQ=255)                                                                                                                                       tCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCgg            <  1:549260/150‑1 (MQ=255)                                                                                                                                                cGGTACTGGCCCGCTGGCCTGGACTGGGAGGTTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTTCGTTTCTGCTTGTCTCTTTGCCGGGCAAGTGGc   >  2:233499/1‑150 (MQ=255)                                                                                                                                                cGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGc   <  1:894761/150‑1 (MQ=255)                                                                                                                                                 ggTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCt  <  1:598857/150‑1 (MQ=255)                                                                                                                                                 ggTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCt  <  1:1111540/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCCTTGTTGCTAGTGCGCCTGTGTTCGGCATTACGTGGCGTGTTGCGCCGTCGCCTTCCCCCCCGTTACCTGAAACCGCTGGGCGTACGCCGCCGCGCTGCACGCCAGGAACCCCAGAATGACTAATACCACCATCACCCTCCCGGTACTGGCCCGCTGGCCTGGACTGGGAGGGTGGAACCTCTATTTTTTTGCAAAGTTTCTGCTGGTCGCGCTGGGCATGCTCGACTTCCAGGCGCTGCCGAACCTGTTGTTCGCTGCGTTTCTGCTTGTCCCTTTGCCGGGCAAGTGGCT  >  NC_002947/3010865‑3011158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑