Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,146,654 T→C 7.0% intergenic (+47/+19) PP_2761 → / ← sfnA ribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,146,6540TC 7.0% 154.2 / 2.6 57intergenic (+47/+19)PP_2761/sfnAribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein
Reads supporting (aligned to +/- strand):  ref base T (27/26);  new base C (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

TTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTT  >  NC_002947/3146520‑3146798
                                                                                                                                      |                                                                                                                                                
tttCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATg                                                                                                                                   >  1:1118533/1‑150 (MQ=255)
      ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCa                                                                                                                             <  2:360571/150‑1 (MQ=255)
      ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCa                                                                                                                             <  1:373060/150‑1 (MQ=255)
      ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCa                                                                                                                             <  1:1871409/150‑1 (MQ=255)
      ctGGCAGCAGGTGGCCACCGGAGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACCAGGCCTGGGGTATGCCCCAGCCCATGCTTTcc                                                                                                                             >  1:699092/1‑149 (MQ=255)
       tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGGTTAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                            <  1:1610720/150‑1 (MQ=255)
         gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCCGGCCATGCTTTCAggc                                                                                                                          >  1:681787/1‑149 (MQ=255)
          cagcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAg                                                                                                                                         <  2:362472/134‑1 (MQ=255)
          cagcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAg                                                                                                                                         >  1:362472/1‑134 (MQ=255)
           agcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACt                                                                                                                        >  1:285446/1‑150 (MQ=255)
                gTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGc                                                                                                                                       >  2:698334/1‑130 (MQ=255)
                gTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGc                                                                                                                                       <  1:698334/130‑1 (MQ=255)
                 tctccACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCGCCAGGACATGCTTTCAGGACTGCTTgg                                                                                                                  <  1:648179/147‑1 (MQ=255)
                 tGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCttt                                                                                                                               <  2:1596714/137‑1 (MQ=255)
                 tGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCttt                                                                                                                               >  1:1596714/1‑137 (MQ=255)
                     cACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGAGTACCGGTCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCATGCCATGCTTTCAGGACTGCTTGGCaga                                                                                                              >  2:648179/1‑148 (MQ=255)
                        cGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGcc                                                                                                           >  1:1294233/1‑150 (MQ=255)
                        cGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGCATACCCCAg                                                                                                                                         <  2:1610930/120‑1 (MQ=255)
                        cGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGATTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGCATACCCCAg                                                                                                                                         >  1:1610930/1‑120 (MQ=255)
                          gCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCAc                                                                                                         <  1:1723664/150‑1 (MQ=255)
                                              ctgctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGggc                                                                                     <  2:1294233/150‑1 (MQ=255)
                                                gctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGggcgg                                                                                   >  2:422932/1‑150 (MQ=255)
                                                gctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGCATACCCCAGGc                                                                                                                                       <  1:378376/98‑1 (MQ=255)
                                                gctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGACCTGGGGCATACCCCAGGc                                                                                                                                       >  2:378376/1‑98 (MQ=255)
                                                    ttCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCg                                                                               >  1:396290/1‑150 (MQ=255)
                                                    ttCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCg                                                                               >  1:1487841/1‑150 (MQ=255)
                                                      cAGTTTTGCCACGCGGCGACTCGGCGCTGGATAGTTCTGGCGTACCCGGCAGTCATAATGTAATTCGACGACTTCGGGCGTACGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCt                                                                             <  1:628813/150‑1 (MQ=255)
                                                        gTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGc                                                                           <  2:1810373/150‑1 (MQ=255)
                                                           ttGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCAt                                                                        <  1:153887/150‑1 (MQ=255)
                                                               cAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTACCATTGAc                                                                    <  1:422932/150‑1 (MQ=255)
                                                                   cGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGc                                                                <  1:524752/150‑1 (MQ=255)
                                                                          tCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGc                                                                                                                                       >  2:1783189/1‑72 (MQ=255)
                                                                          tCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGc                                                                                                                                       <  1:1783189/72‑1 (MQ=255)
                                                                             gcgcTGGATTGTTTTGGCGTTCCCGGCGGAACGCCAGTGGCGGTACGAGGCCTGGGGTTTGCCCCAGGCCATGCTTTCAGGACTGCTTGGCCCCCCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCga                                                      <  2:1689626/150‑1 (MQ=255)
                                                                                 tGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCgatgat                                                  <  1:948338/150‑1 (MQ=255)
                                                                                   gATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGc                                                >  1:402918/1‑150 (MQ=255)
                                                                                    aTAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCg                                               >  2:1402698/1‑150 (MQ=255)
                                                                                       gTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTc                                            >  2:915319/1‑150 (MQ=255)
                                                                                        ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTcc                                           >  1:308808/1‑150 (MQ=255)
                                                                                         acTGGCGTTCCGCCCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATCCACAGGGCCGGTGACTCGCATGGACTGTCCATTCTCCGATGATGcagatcg                                          >  2:18436/2‑144 (MQ=255)
                                                                                           tGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCca                                                                                               <  1:1177588/95‑1 (MQ=255)
                                                                                           tGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCca                                                                                               >  2:1177588/1‑95 (MQ=255)
                                                                                            ggCGTTCCGGGCGGAACACCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGt                                       <  2:1149076/150‑1 (MQ=255)
                                                                                                    gggCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATAAcc                               >  1:271098/1‑150 (MQ=255)
                                                                                                     ggCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCg                              >  1:1185393/1‑150 (MQ=255)
                                                                                                       cGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCggg                            <  1:1402698/150‑1 (MQ=255)
                                                                                                        ggAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCa                                 >  2:1327234/1‑144 (MQ=255)
                                                                                                        ggAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCa                                 <  1:1327234/144‑1 (MQ=255)
                                                                                                          aaCGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCTATGATGCGTTCCTTGTAGATCACCGGGTtg                         >  2:345215/1‑150 (MQ=255)
                                                                                                          aaCGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTtg                         >  1:747831/1‑150 (MQ=255)
                                                                                                             gCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGc                      <  2:1185393/150‑1 (MQ=255)
                                                                                                                    tCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa                                      >  1:988595/1‑127 (MQ=255)
                                                                                                                    tCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa                                      <  2:988595/127‑1 (MQ=255)
                                                                                                                    tCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAggcgg               >  2:386010/1‑150 (MQ=255)
                                                                                                                         aCGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTa          <  2:402918/150‑1 (MQ=255)
                                                                                                                                cTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGt   >  1:914255/1‑150 (MQ=255)
                                                                                                                                 tGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGtt  <  1:915319/150‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                
TTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTT  >  NC_002947/3146520‑3146798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑