Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,793,940 A→T 6.5% I209N (ATT→AAT)  PP_3355 ← beta‑ketothiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,793,9400AT 6.5% 244.4 / 7.4 77I209N (ATT→AAT) PP_3355beta‑ketothiolase
Reads supporting (aligned to +/- strand):  ref base A (32/40);  new base T (3/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GCCGCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCATAG  >  NC_002947/3793796‑3794089
                                                                                                                                                |                                                                                                                                                     
gccgcGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCcg                                                                                                                                                  >  1:903113/1‑150 (MQ=255)
 ccgcGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCcgc                                                                                                                                                 >  1:42122/1‑150 (MQ=255)
   gcgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGt                                                                                                                                               <  2:1167576/150‑1 (MQ=255)
       cATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGACTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggct                                                                                                                                           <  2:1404787/150‑1 (MQ=255)
          cGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggctggc                                                                                                                                        <  1:1731323/150‑1 (MQ=255)
             ccaccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGc                                                                                                                                     <  2:1095623/150‑1 (MQ=255)
              caccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGcc                                                                                                                                    >  2:1613654/1‑150 (MQ=255)
                ccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTCTGCCGTGGGTGGCTGTCCTGGCTGCACTCACACACTGCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCtt                                                                                                                                  >  1:434086/1‑150 (MQ=255)
                   cgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTa                                                                                                                               <  2:1918780/150‑1 (MQ=255)
                   cgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCAGTGGGTGGCTGTCCTGGCTCAGCTCAATCACCGCACCCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTa                                                                                                                               <  1:339188/150‑1 (MQ=255)
                    gcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTAc                                                                                                                              <  2:578905/150‑1 (MQ=255)
                       aCTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTc                                                                                                                           >  2:742339/1‑150 (MQ=255)
                       aCTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTc                                                                                                                           <  2:42122/150‑1 (MQ=255)
                               cAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCt                                                                                                                   <  1:1856368/150‑1 (MQ=255)
                                  ccGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTgcg                                                                                                                >  2:77037/1‑150 (MQ=255)
                                    gTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGcgcg                                                                                                              <  2:239997/150‑1 (MQ=255)
                                     tCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCa                                                                                                                         >  2:1269262/1‑138 (MQ=255)
                                     tCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCa                                                                                                                         <  1:1269262/138‑1 (MQ=255)
                                      cTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGt                                                                                                            <  2:1123262/150‑1 (MQ=255)
                                      cTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGt                                                                                                            <  1:623311/150‑1 (MQ=255)
                                        gCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGa                                                                                                          <  2:1599838/150‑1 (MQ=255)
                                        gCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGa                                                                                                          <  2:865801/150‑1 (MQ=255)
                                           cGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGc                                                                                                       <  1:1118203/150‑1 (MQ=255)
                                             ccACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTg                                                                                                     <  2:1707934/150‑1 (MQ=255)
                                              cACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGa                                                                                                    <  1:1456420/150‑1 (MQ=255)
                                              cACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGa                                                                                                    <  1:573102/150‑1 (MQ=255)
                                               aCCGGCGTGCACTGGGCGCAGTCGGGCGAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTGCTGGCTGACGTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTACAGGGCCTGCGCGGTGACGCTGAc                                                                                                   <  1:1516057/150‑1 (MQ=255)
                                                 cGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGGCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGa                                                                                                 >  2:911150/1‑150 (MQ=255)
                                                   gCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGAtt                                                                                               >  2:1451231/1‑150 (MQ=255)
                                                   gCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGAtt                                                                                               <  1:742339/150‑1 (MQ=255)
                                                     gTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGc                                                                                                                                     <  2:1041990/110‑1 (MQ=255)
                                                     gTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGc                                                                                                                                     >  1:1041990/1‑110 (MQ=255)
                                                        cACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtc                                                                                          <  1:675789/150‑1 (MQ=255)
                                                         aCCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGTCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcg                                                                                         >  2:1254640/1‑150 (MQ=255)
                                                          ccGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                        <  2:178719/150‑1 (MQ=255)
                                                               cgcAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAAc                                                                                   <  2:303429/150‑1 (MQ=255)
                                                                 cAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCa                                                                                 >  1:640476/1‑150 (MQ=255)
                                                                  aGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAg                                                                                >  2:1315588/1‑150 (MQ=255)
                                                                    tCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGcc                                                                              <  1:77037/150‑1 (MQ=255)
                                                                      gggCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCt                                                                            >  1:283920/1‑150 (MQ=255)
                                                                         ccAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAACCCTGcc                                                                         >  2:1940256/1‑149 (MQ=255)
                                                                              gCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                    <  2:283920/150‑1 (MQ=255)
                                                                                    gcgTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGc                                                              <  2:1355772/150‑1 (MQ=255)
                                                                                          gTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtc                                                                                          <  1:1866112/116‑1 (MQ=255)
                                                                                          gTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtc                                                                                          >  2:1866112/1‑116 (MQ=255)
                                                                                              gCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCtt                                                                >  1:912769/1‑138 (MQ=255)
                                                                                              gCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCtt                                                                <  2:912769/138‑1 (MQ=255)
                                                                                                   gggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATgg                                               <  2:434086/150‑1 (MQ=255)
                                                                                                     gtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCt                                             >  2:213058/1‑150 (MQ=255)
                                                                                                     gtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCt                                             <  1:1613654/150‑1 (MQ=255)
                                                                                                          tGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCa                                        >  1:295364/1‑150 (MQ=255)
                                                                                                            tCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAgg                                      >  1:1395808/1‑150 (MQ=255)
                                                                                                             ccTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAggg                                     <  1:1451231/150‑1 (MQ=255)
                                                                                                               tGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGcc                                   >  2:177762/1‑150 (MQ=255)
                                                                                                                  cTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCAc                                <  1:1914187/150‑1 (MQ=255)
                                                                                                                  cTGACCTCACTCACGCCACGCGGCAAGGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTAGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCAc                                <  2:9631/150‑1 (MQ=255)
                                                                                                                      ccTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGcgcg                                                                                                              <  1:1294385/68‑1 (MQ=255)
                                                                                                                      ccTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGcgcg                                                                                                              >  2:1294385/1‑68 (MQ=255)
                                                                                                                       ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                    <  2:1597353/109‑1 (MQ=255)
                                                                                                                       ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                    >  1:1597353/1‑109 (MQ=255)
                                                                                                                       ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTc                           >  2:48832/1‑150 (MQ=255)
                                                                                                                         cactcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCa                         >  1:1186208/1‑150 (MQ=255)
                                                                                                                              acgccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGtt                    >  1:951627/1‑150 (MQ=255)
                                                                                                                               cgccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGttt                   >  2:1514806/1‑150 (MQ=255)
                                                                                                                                gccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTc                  <  2:951627/150‑1 (MQ=255)
                                                                                                                                gccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATAGCTGCTCCGGGCCCACCCGTCCACCGTTTc                  >  2:245153/1‑150 (MQ=255)
                                                                                                                                   acgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGcgcg                                                                                                              <  2:1765878/55‑1 (MQ=25)
                                                                                                                                   acgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGcgcg                                                                                                              >  1:1765878/1‑55 (MQ=25)
                                                                                                                                   acgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTc                                                                                             >  2:1076047/1‑72 (MQ=38)
                                                                                                                                   acgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTc                                                                                             <  1:1076047/72‑1 (MQ=38)
                                                                                                                                   acgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCAcg               >  1:266484/1‑150 (MQ=255)
                                                                                                                                   acgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCAcg               >  1:59383/1‑150 (MQ=255)
                                                                                                                                     gcgGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                        >  1:1704094/1‑75 (MQ=255)
                                                                                                                                     gcgGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                        <  2:1704094/75‑1 (MQ=255)
                                                                                                                                     gcgGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATgg                                               <  1:136087/116‑1 (MQ=255)
                                                                                                                                     gcgGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATgg                                               >  2:136087/1‑116 (MQ=255)
                                                                                                                                       ggCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGcc           >  2:1579099/1‑150 (MQ=255)
                                                                                                                                             gcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCa     <  1:1315588/150‑1 (MQ=255)
                                                                                                                                               aatgcCGCGTGGCTGGCCGCCTTCTACCGCCAGTGCCTGCGCGGTGACGCGGACGACTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCATa   <  2:903113/150‑1 (MQ=255)
                                                                                                                                                atgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGCCCACCGTTTCACGCGCCAGGCCATAg  <  1:1254640/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GCCGCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCATAG  >  NC_002947/3793796‑3794089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑