Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,094,380	G→T	6.4%	G445W (GGG→TGG)	PP_3602 →	2,5‑dioxovalerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,094,380	0	G	T	6.4%	191.8 / 2.3	62	G445W (GGG→TGG)	PP_3602	2,5‑dioxovalerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base T (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

ACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGGTGTGCTAC  >  NC_002947/4094233‑4094526                                                                                                                                                    |                                                                                                                                                    accaccGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCggg                                                                                                                                                   <  2:1037656/150‑1 (MQ=255) accaccGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCggg                                                                                                                                                   >  2:1410812/1‑150 (MQ=255)  ccaccGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGc                                                                                                                                                  <  1:829183/150‑1 (MQ=255)  ccaccGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGc                                                                                                                                                  <  1:1680845/150‑1 (MQ=255)  ccaccGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCCTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGc                                                                                                                                                  <  2:1028161/150‑1 (MQ=255)                       aGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTAcc                                                                                                                             >  2:1421192/1‑150 (MQ=255)                         gACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCg                                                                                                                           >  2:1896498/1‑150 (MQ=255)                           cAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGAc                                                                                                                         >  2:1739240/1‑150 (MQ=255)                            aaCGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCTGGCGAATCCTGGTCAATGGCTAc                                                                                                                              <  2:165876/144‑1 (MQ=255)                            aaCGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCTGGCGAATCCTGGTCAATGGCTAc                                                                                                                              >  1:165876/1‑144 (MQ=255)                            aaCGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGAcc                                                                                                                        <  2:1445548/150‑1 (MQ=255)                               gACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATgg                                                                                                                                  >  2:1529182/1‑137 (MQ=255)                               gACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATgg                                                                                                                                  <  1:1529182/137‑1 (MQ=255)                                 ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACCTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCTGGCGAATCCTGGTCAATGGCTACCCGACCGgtgt                                                                                                                   >  2:147390/1‑150 (MQ=255)                                  cAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTc                                                                                                                  >  1:79073/1‑150 (MQ=255)                                   aaCTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCg                                                                                                                 >  1:1918444/1‑150 (MQ=255)                                     cTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGaa                                                                                                               <  2:948660/150‑1 (MQ=255)                                         gcgACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCgg                                                                                                                                                    >  2:1232684/1‑109 (MQ=255)                                         gcgACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCgg                                                                                                                                                    <  1:1232684/109‑1 (MQ=255)                                           gACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACGCGACAGGTGTCGAAGTCtgt                                                                                                         >  2:22951/1‑150 (MQ=255)                                            aCGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCtgtg                                                                                                        >  1:321536/1‑150 (MQ=255)                                             cgcgCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGACCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGa                                                                                                       <  1:1272788/150‑1 (MQ=255)                                                gctgctCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTAccc                                                                                                                            >  2:1083786/1‑126 (MQ=255)                                                gctgctCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTAccc                                                                                                                            <  1:1083786/126‑1 (MQ=255)                                                   gctCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAt                                                                                                 <  2:984628/150‑1 (MQ=255)                                                     tCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑gg                                                                                              >  1:575676/1‑150 (MQ=255)                                                      cGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGt                                                                                             <  1:1364523/150‑1 (MQ=255)                                                         ccTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGTCGGAGGAGAATGCAGCGCGAATCCCGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCa                                                                                          >  1:1684986/1‑150 (MQ=255)                                                                       tGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTAcc                                                                            <  1:1517718/150‑1 (MQ=255)                                                                          ccGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCgg                                                                         <  1:1067920/150‑1 (MQ=255)                                                                                    gATCGGCGAAGCGGAGTACTTCGAGGCCTTCGCCGGGCTGGTGGCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAACGGCTATCCGACCGGTGCCGAAGTCTGTGAT‑CCATATTTGCACGCCGGCCCGTACCCGGCAAACTCCGa                                                               <  2:705877/150‑1 (MQ=255)                                                                                    gATCGGCGAAGCGGAGGGCTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGa                                                               <  2:1400571/150‑1 (MQ=255)                                                                                    gATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGCCCTCCgc                                                               >  1:1708898/1‑149 (MQ=255)                                                                                    gATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGCGATGCCAt                                                                                                 >  1:383364/1‑117 (MQ=255)                                                                                    gATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGCGATGCCAt                                                                                                 <  2:383364/117‑1 (MQ=255)                                                                                                ggaggaCTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCAc                                                   <  2:575676/150‑1 (MQ=255)                                                                                                   ggaCTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCa                                                                                                  <  2:838465/101‑1 (MQ=255)                                                                                                   ggaCTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCa                                                                                                  >  1:838465/1‑101 (MQ=255)                                                                                                    gaCTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGGCCTCCGATGCGCGTGGCACCtcg                                               >  2:1553071/1‑150 (MQ=255)                                                                                                    gaCTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCtcg                                               <  1:1410812/150‑1 (MQ=255)                                                                                                       ttCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATg                                                                                                     <  2:561145/94‑1 (MQ=255)                                                                                                       ttCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATg                                                                                                     >  1:561145/1‑94 (MQ=255)                                                                                                       ttCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCtcggtc                                            <  1:1421192/150‑1 (MQ=255)                                                                                                       ttCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCtcggtc                                            <  1:1535867/150‑1 (MQ=255)                                                                                                          gATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGc                                                                                                    >  1:26019/1‑92 (MQ=255)                                                                                                          gATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGc                                                                                                    <  2:26019/92‑1 (MQ=255)                                                                                                          gATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGc                                         >  2:716799/1‑150 (MQ=255)                                                                                                                        gctggTGCCGCTGCTGGAGGAGAAAGTCTGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGTGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGa                           <  1:147390/150‑1 (MQ=255)                                                                                                                            gTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGa                                                                                                       >  1:1900775/1‑71 (MQ=255)                                                                                                                            gTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGa                                                                                                       <  2:1900775/71‑1 (MQ=255)                                                                                                                            gTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGc                       >  2:670397/1‑150 (MQ=255)                                                                                                                                  ctgctgGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCAc                                                   >  1:1836386/1‑116 (MQ=255)                                                                                                                                  ctgctgGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCAc                                                   <  2:1836386/116‑1 (MQ=255)                                                                                                                                   tgctgGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTgc                >  1:154455/1‑150 (MQ=255)                                                                                                                                      tgGAGGAGAAAGTCGGGCGAATCCTGGTTAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGcc             <  2:794341/150‑1 (MQ=255)                                                                                                                                      tgGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGcc             >  2:581251/1‑150 (MQ=255)                                                                                                                                       ggaggaGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTg                                   <  2:1843447/127‑1 (MQ=255)                                                                                                                                       ggaggaGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTg                                   >  1:1843447/1‑127 (MQ=255)                                                                                                                                          ggagAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCTGCACTTTGGCCATCGACCGCTTCCTGCGCCCGgt         <  2:321536/150‑1 (MQ=255)                                                                                                                                          ggagAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGgt         <  2:1684986/150‑1 (MQ=255)                                                                                                                                           gagaAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGgtg        <  1:1739240/150‑1 (MQ=255)                                                                                                                                            agaAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGgtgt       >  2:291505/1‑150 (MQ=255)                                                                                                                                            agaAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTAGGTCGGCACTTTGGCCATAGACAGCTTCCTGCGCCCGgtgt       >  2:547114/1‑150 (MQ=255)                                                                                                                                              aaaGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCGGATGCGCGTGGCACCTCGGTCGGAACTTTGGCAAACGACCGCTTCCTTCGCCCGGTGTGc     >  1:1683419/1‑150 (MQ=37)                                                                                                                                                 gTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCCT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGGTGTGCTAc  >  2:524762/1‑150 (MQ=255)                                                                                                                                                 gTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGGTGTGCTAc  <  2:1708898/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCAT‑GGTGCACGGCGGCCCGTACCCGGCGACCTCCGATGCGCGTGGCACCTCGGTCGGCACTTTGGCCATCGACCGCTTCCTGCGCCCGGTGTGCTAC  >  NC_002947/4094233‑4094526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑