Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,281,408 G→T 5.9% I20I (ATC→ATA)  PP_3751 ← cyanate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,281,4080GT 5.9% 209.7 / 2.9 68I20I (ATC→ATA) PP_3751cyanate transporter
Reads supporting (aligned to +/- strand):  ref base G (21/43);  new base T (2/2);  total (23/45)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

CCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAGAGATTGGATGCAGGT  >  NC_002947/4281269‑4281550
                                                                                                                                           |                                                                                                                                              
cccAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTACCGGGCCGATACTGGTCAGGATCGGCCGCa                                                                                                                                      <  1:545871/150‑1 (MQ=255)
 ccAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAg                                                                                                                                     <  2:73354/150‑1 (MQ=255)
  cAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAgg                                                                                                                                    <  2:37467/150‑1 (MQ=255)
   aGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGt                                                                                                                                   <  1:1570715/150‑1 (MQ=255)
    gCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGtt                                                                                                                                  <  2:632452/150‑1 (MQ=255)
       tCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACCGGCCGATACTGGTCAGGATCGGCCGCAGGTTCaa                                                                                                                               <  2:699108/150‑1 (MQ=255)
          cAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGc                                                                                                                            >  2:213725/1‑150 (MQ=255)
            gCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCca                                                                                                                          <  2:32150/150‑1 (MQ=255)
               aCGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCcagca                                                                                                                       >  1:871164/1‑150 (MQ=255)
               aCGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCcagca                                                                                                                       >  2:1630682/1‑150 (MQ=255)
               aCGGTTGCAACAGGGGCACCAGGCCAATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCaa                                                                                                                               <  2:946633/142‑1 (MQ=255)
               aCGGTTGCAACAGGGGCACCAGGCCAATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCaa                                                                                                                               >  1:946633/1‑142 (MQ=255)
                cGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCcagcag                                                                                                                      <  2:1908727/150‑1 (MQ=255)
                 ggTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAgg                                                                                                                     <  1:400016/150‑1 (MQ=255)
                 ggTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGCCAGGATCGGCCGCAGGTTCAACGCCAGCAgg                                                                                                                     <  1:1804792/150‑1 (MQ=255)
                   ttGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGAt                                                                                                                   <  1:1467823/150‑1 (MQ=255)
                       aaCAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCAcc                                                                                                               <  1:1375894/150‑1 (MQ=255)
                        aCAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCa                                                                                                              >  1:913363/1‑150 (MQ=255)
                        aCAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCa                                                                                                              >  2:622709/1‑150 (MQ=255)
                         cAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAg                                                                                                             <  1:213725/150‑1 (MQ=255)
                                   aGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAACAACAGGTTc                                                                                                   <  2:373573/150‑1 (MQ=255)
                                       ccATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTa                                                                                               <  1:1630682/150‑1 (MQ=255)
                                        cATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCa                                                                                                                 <  1:217600/131‑1 (MQ=255)
                                        cATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCa                                                                                                                 >  2:217600/1‑131 (MQ=255)
                                         aTGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAATAGGTTCAGTACa                                                                                             <  2:716413/150‑1 (MQ=255)
                                             acaGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGtt                                                                                                                                  <  2:1224156/109‑1 (MQ=255)
                                             acaGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGtt                                                                                                                                  >  1:1224156/1‑109 (MQ=255)
                                                  aCCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCAtt                                                                                    <  1:1348278/150‑1 (MQ=255)
                                                    cGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATtgt                                                                                  <  2:1447177/150‑1 (MQ=255)
                                                    cGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATtgt                                                                                  <  1:622709/150‑1 (MQ=255)
                                                      gTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGa                                                                                <  1:1448071/150‑1 (MQ=255)
                                                      gTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGa                                                                                >  1:1926936/1‑150 (MQ=255)
                                                         gcgcCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGTGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCa                                                                                                                                      <  1:1179278/93‑1 (MQ=255)
                                                         gcgcCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGTGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCa                                                                                                                                      >  2:1179278/1‑93 (MQ=255)
                                                           gcCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCgg                                                                           <  2:871164/150‑1 (MQ=255)
                                                           gcCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCgg                                                                           >  2:1322228/1‑150 (MQ=255)
                                                                cAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTtctc                                                                      <  2:633837/150‑1 (MQ=255)
                                                                cAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTtctc                                                                      <  1:885959/150‑1 (MQ=255)
                                                                cAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTtctc                                                                      <  2:1807445/150‑1 (MQ=255)
                                                                    aaGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATCCTGACCAGTATCGGcccgt                                                                                                                                      >  1:48917/1‑79 (MQ=25)
                                                                    aaGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATCCTGACCAGTATCGGcccgt                                                                                                                                      <  2:48917/82‑4 (MQ=25)
                                                                    aaGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATCCTGACCAGTATCGGc                                                                                                                                          >  1:1401718/1‑78 (MQ=37)
                                                                    aaGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATCCTGACCAGTATCGGc                                                                                                                                          <  2:1401718/78‑1 (MQ=37)
                                                                    aaGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCCAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGt                                                                  <  2:1508469/150‑1 (MQ=255)
                                                                        ccgccTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACa                                                              >  2:1899113/1‑150 (MQ=255)
                                                                         cgccTGCGGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAg                                                             <  2:176871/150‑1 (MQ=255)
                                                                          gccTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAgg                                                            <  2:415038/150‑1 (MQ=255)
                                                                                tgGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTgg                                                      <  2:1926936/150‑1 (MQ=255)
                                                                                 ggTAACCCAGGCCGGTGCTGGCGCGCATGGGTTGGGGGAACGGGCAGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTTAGTCGGTTCTCTTGTAACAGGAACTGGc                                                     <  2:907981/150‑1 (MQ=255)
                                                                                 ggTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGc                                                     <  2:1306024/150‑1 (MQ=255)
                                                                                   tAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCa                                                   <  1:1899113/150‑1 (MQ=255)
                                                                                          ggCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATccc                                            <  2:306238/150‑1 (MQ=255)
                                                                                           gCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACa                                                              >  1:585533/1‑131 (MQ=255)
                                                                                           gCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACa                                                              <  2:585533/131‑1 (MQ=255)
                                                                                           gCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCcg                                           <  2:913363/150‑1 (MQ=255)
                                                                                           gCCGGTGCTGGCGCGCATAGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCAcc                                                                                                               <  1:87305/82‑1 (MQ=255)
                                                                                           gCCGGTGCTGGCGCGCATAGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCAcc                                                                                                               >  2:87305/1‑82 (MQ=255)
                                                                                            ccGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCAtt                                                 <  1:179802/143‑1 (MQ=255)
                                                                                            ccGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCAtt                                                 >  2:179802/1‑143 (MQ=255)
                                                                                                   tGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCa                                                                                                                                      >  2:863810/1‑51 (MQ=255)
                                                                                                   tGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCa                                                                                                                                      <  1:863810/51‑1 (MQ=255)
                                                                                                   tGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATtg                                                                                   <  1:1644817/102‑1 (MQ=255)
                                                                                                   tGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATtg                                                                                   >  2:1644817/1‑102 (MQ=255)
                                                                                                      cgcgcATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGctggc                                <  1:1811525/150‑1 (MQ=255)
                                                                                                               gTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGaa                       >  1:670702/1‑150 (MQ=255)
                                                                                                                    aGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGaa                  >  1:145982/1‑150 (MQ=255)
                                                                                                                         cGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTCGCCTGGCAGAAATGAAGAGAt             >  2:1596108/1‑150 (MQ=255)
                                                                                                                                    tGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGCAATGAAGAGATTGGATGCAGGt  >  2:1088799/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                              
CCCAGCGTCGCAGCCACGGTTGCAACAGGGGCACCAGGCCCATGCACAGCACCGGTAGCGCCGTCAGCAAGGCCGCCTGCTGGTAACCCAGGCCGGTGCTGGCGCGCATGGGTTCGAGTAACGGGCCGATACTGGTCAGGATCGGCCGCAGGTTCAACGCCAGCAGGATCACCAGCAACAGGTTCAGTACAGCGCTCATTGTGAGTCGGTTCTCTTGTAACAGGAACTGGCCATTATCCCGGCTCGGCTGGCCTGGCAGAAATGAAGAGATTGGATGCAGGT  >  NC_002947/4281269‑4281550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑