Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,479,857 A→G 5.1% P388P (CCA→CCG)  ybdR → Zn‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,479,8570AG 5.1% 238.2 / 2.0 78P388P (CCA→CCG) ybdRZn‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base G (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

CCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGA  >  NC_002947/4479710‑4480001
                                                                                                                                                   |                                                                                                                                                
ccTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGCCATCCTCGTGCCAgg                                                                                                                                                >  2:721489/1‑150 (MQ=255)
 cTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTGGATCAAAAGCAGGAGATCTGCCGCAAGGTCATCCTCGTGGCAGGt                                                                                                                                               >  2:1288045/1‑150 (MQ=255)
 cTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGt                                                                                                                                               >  1:1588840/1‑150 (MQ=255)
     ccGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgctt                                                                                                                                           >  1:743219/1‑149 (MQ=255)
                 aGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCAcat                                                                                                                               <  2:1167593/150‑3 (MQ=255)
                  gCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCACCTGGCAcgag                                                                                                                              <  2:1588840/150‑4 (MQ=255)
                  gCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCtt                                                                                                                              <  2:1654121/150‑1 (MQ=255)
                   cacaTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTg                                                                                                                             <  2:1230694/150‑1 (MQ=255)
                      aTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCAcctgg                                                                                                                                    >  2:1716105/1‑137 (MQ=255)
                      aTCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCGGGTGCAGCACCTgg                                                                                                                                    <  1:1716105/140‑1 (MQ=255)
                      aTCGAAGCAGGGCGCCTGCAAACGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGc                                                                                                                          >  1:1221999/1‑150 (MQ=255)
                        cGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGCGCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGccc                                                                                                                        >  1:1475249/1‑150 (MQ=255)
                         gAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGCCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCt                                                                                                                       <  1:721489/150‑1 (MQ=255)
                         gAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTGAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCt                                                                                                                       <  1:1288045/150‑1 (MQ=255)
                            gCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGac                                                                                                                    >  1:947655/1‑150 (MQ=255)
                               gggCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGGGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGaccac                                                                                                                 <  1:1087811/150‑1 (MQ=255)
                               cggCGCCTGCATACGGAACTGCTGGTTCCTCCCCTCTTAGCCTTAGAGGCGGCGGCCCTGGCCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGCCATCCTCGTCCCAGGTGCTGCACTCGGCACCTTGGGCCCTGaccac                                                                                                                 <  2:362499/149‑1 (MQ=255)
                                  cgcCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACctc                                                                                                              >  1:277932/1‑150 (MQ=255)
                                      tGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTaga                                                                                                          <  2:1539663/150‑1 (MQ=255)
                                         aaCCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAgagga                                                                                                       <  1:909140/150‑1 (MQ=255)
                                            cGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAggagga                                                                                                    <  2:1221999/150‑1 (MQ=255)
                                             ggAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGaa                                                                                                   >  1:1091664/1‑150 (MQ=255)
                                              gAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAAt                                                                                                  <  1:1656524/150‑1 (MQ=255)
                                               aaCTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGc                                                                                                                          <  1:1698021/125‑1 (MQ=255)
                                               aaCTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGc                                                                                                                          >  2:1698021/1‑125 (MQ=255)
                                               aaCTGATCGTTACTCACCGCTTAGCCCTAGAAGACGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGt                                                                                                                                               >  2:854411/1‑104 (MQ=255)
                                               aaCTGATCGTTACTCACCGCTTAGCCCTAGAAGACGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGt                                                                                                                                               <  1:854411/104‑1 (MQ=255)
                                                    aTCGTTACACACCGCATAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTAGATGGGCGACGACCGGGCTTGCGCACCGTCATCCTCATGCCAGGTGCTGCACCCCGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCAt                                                                                            <  1:442668/150‑1 (MQ=255)
                                                        ttACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAAc                                                                                        >  1:1449645/1‑150 (MQ=255)
                                                           cTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGt                                                                                     >  2:889824/1‑150 (MQ=255)
                                                            tCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCAcga                                                                                                                               >  1:880268/1‑105 (MQ=255)
                                                            tCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCAcga                                                                                                                               <  2:880268/107‑3 (MQ=255)
                                                              aCCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGtctc                                                                                  >  1:1096774/1‑150 (MQ=255)
                                                              aCCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGtctc                                                                                  <  2:659493/150‑1 (MQ=255)
                                                              aCCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGtctc                                                                                  >  1:341820/1‑150 (MQ=255)
                                                               ccGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTg                                                                                                                             >  1:6733/1‑106 (MQ=255)
                                                               ccGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTg                                                                                                                             <  2:6733/106‑1 (MQ=255)
                                                                  cTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCCTGAAGTGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCa                                                                              >  1:610982/1‑150 (MQ=255)
                                                                        ccTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACt                                                                        >  1:904099/1‑150 (MQ=255)
                                                                        ccTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACt                                                                        >  2:1434496/1‑150 (MQ=255)
                                                                         cTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACtt                                                                       >  2:407205/1‑150 (MQ=255)
                                                                           agaagaGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCt                                                                     <  2:430362/150‑1 (MQ=255)
                                                                               gagGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGAt                                                                 >  1:142437/1‑150 (MQ=255)
                                                                                agGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgct                                                                                                                                            >  1:1480603/1‑74 (MQ=255)
                                                                                agGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGtgct                                                                                                                                            <  2:1480603/74‑1 (MQ=255)
                                                                                agGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGaccacc                                                                                                                >  2:598891/1‑102 (MQ=255)
                                                                                agGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGaccacc                                                                                                                <  1:598891/102‑1 (MQ=255)
                                                                                  gctgcCATGGGCTACAAAATGTTCGATCAAAAGCAGTGTAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGCGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGc                                                              <  1:101013/147‑1 (MQ=255)
                                                                                   cggcCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGcc                                                             <  2:142437/150‑1 (MQ=255)
                                                                                       cATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACAACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAg                                                         >  2:732584/1‑150 (MQ=255)
                                                                                        aTGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGc                                                        <  2:1786146/150‑1 (MQ=255)
                                                                                            gCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCa                                                    >  1:1286673/1‑150 (MQ=255)
                                                                                                 agaaTGGTCGGTCGAAGGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGGGCAGCAATGATCATGAACAGTCTCGCCAGTTCCTCCTGGATTACCGTAGCGGCAAttct                                               >  2:181550/3‑150 (MQ=255)
                                                                                                    aTGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATtcttct                                            <  1:1621728/150‑1 (MQ=255)
                                                                                                          gATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTATAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCAt                                      >  2:103217/1‑150 (MQ=255)
                                                                                                           aTCCAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTagag                                                                                                         <  2:558825/82‑1 (MQ=255)
                                                                                                           aTCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTagag                                                                                                         >  1:558825/1‑82 (MQ=255)
                                                                                                            tCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTGCTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCg                                    >  1:40436/1‑150 (MQ=255)
                                                                                                               aaaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGAcc                                 <  1:889824/150‑1 (MQ=255)
                                                                                                               aaaGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGAcc                                 >  1:1548887/1‑150 (MQ=255)
                                                                                                                    aGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACATCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTgg                            >  1:42824/1‑150 (MQ=255)
                                                                                                                      gATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGcc                                                             >  2:1360026/1‑115 (MQ=255)
                                                                                                                      gATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGcc                                                             <  1:1360026/115‑1 (MQ=255)
                                                                                                                        tAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTGCTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCa                        <  2:40436/150‑1 (MQ=255)
                                                                                                                         aaCTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCTGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCAt                       >  1:740069/1‑150 (MQ=255)
                                                                                                                              ccGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCa                  <  2:1091664/150‑1 (MQ=255)
                                                                                                                                gCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGc                <  2:1096774/150‑1 (MQ=255)
                                                                                                                                     gTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTAc                                                                         <  1:279484/88‑1 (MQ=255)
                                                                                                                                     gTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTAc                                                                         >  2:279484/1‑88 (MQ=255)
                                                                                                                                      tCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAAttctt                                              >  2:989371/1‑114 (MQ=255)
                                                                                                                                      tCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAAttctt                                              <  1:989371/114‑1 (MQ=255)
                                                                                                                                      tCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTc          >  2:1815911/1‑150 (MQ=255)
                                                                                                                                        aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCgt              >  2:1267912/1‑144 (MQ=255)
                                                                                                                                        aTCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCgt              <  1:1267912/144‑1 (MQ=255)
                                                                                                                                         tCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCgg       >  2:784976/1‑150 (MQ=255)
                                                                                                                                          ccTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGa      >  2:708094/1‑150 (MQ=255)
                                                                                                                                            tCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGc    <  2:947655/150‑1 (MQ=255)
                                                                                                                                              gTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGa  <  1:1434496/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCTTCCCGAACTGCTGGAGCACATCGAAGCAGGGCGCCTGCAACCGGAACTGATCGTTACTCACCGCTTAGCCCTAGAAGAGGCGGCCATGGGCTACAAAATGTTCGATCAAAAGCAGGATAACTGCCGCAAGGTCATCCTCGTGCCAGGTGCTGCACCCGGCACCTTGGGCCCTGACCACCTCTAGAGGAGGAATGATCATGAACGGTCTCGCCAGTTACTTCTGGATTGCCGTAGCGGCAATTCTTCTGCTCATCGACCTGTGGGCCATCGTCAGCGTGTTCCGGAGCGA  >  NC_002947/4479710‑4480001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑