Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,501,341 A→T 5.5% intergenic (‑236/+19) PP_3989 ← / ← PP_t60 DNA‑cytosine methyltransferase/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,501,3410AT 5.5% 231.2 / 4.3 73intergenic (‑236/+19)PP_3989/PP_t60DNA‑cytosine methyltransferase/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base T (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

GAAAAGCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACG  >  NC_002947/4501194‑4501487
                                                                                                                                                   |                                                                                                                                                  
gAAAAGCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGaata                                                                                                                                                  >  2:1000660/1‑150 (MQ=255)
       tctAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAggg                                                                                                                                           >  1:1608789/1‑150 (MQ=255)
       tctAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAggg                                                                                                                                           >  2:831793/1‑150 (MQ=255)
        ctAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGc                                                                                                                                          <  2:1256949/150‑1 (MQ=255)
         tAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGATTATTCAGGGCt                                                                                                                                         <  2:555401/150‑1 (MQ=255)
           ggggAAGGGTGACACGGCAAGCTAAAACAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttt                                                                                                                                       <  2:732832/150‑1 (MQ=255)
           ggggAAGCGCGTAACGCCAAGCTGAAGCAGCTCGCAAACAGCATGTGTCGAATGCGGAAAAAATCCAAAAAATGAGGAAACGACCCGGATAACAGAAGGTAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttt                                                                                                                                       <  1:234687/150‑1 (MQ=255)
             ggAAGAGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttttt                                                                                                                                     <  1:1000660/150‑3 (MQ=255)
                          gCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGCGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGc                                                                                                                        >  2:59839/1‑150 (MQ=255)
                           ccAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCg                                                                                                                       >  1:636917/1‑150 (MQ=255)
                            cAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCt                                                                                                                                         >  1:1187448/1‑131 (MQ=255)
                            cAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCt                                                                                                                                         <  2:1187448/131‑1 (MQ=255)
                             aaGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTa                                                                                                                     <  2:1798116/150‑1 (MQ=255)
                                  gAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGAt                                                                                                                <  1:1787460/150‑1 (MQ=255)
                                        gcTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAAc                                                                                                          >  1:1242064/1‑150 (MQ=255)
                                          tCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttt                                                                                                                                       <  1:1199450/119‑1 (MQ=255)
                                          tCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCttt                                                                                                                                       >  2:1199450/1‑119 (MQ=255)
                                            gCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTa                                                                                                      >  1:1065573/1‑150 (MQ=255)
                                               cacaGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGa                                                                                                   <  2:636917/150‑1 (MQ=255)
                                                          tCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGc                                                                                            <  1:1708994/146‑1 (MQ=255)
                                                          tCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGc                                                                                            >  2:1708994/1‑146 (MQ=255)
                                                              aTGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGAc                                                                                    >  1:1281465/1‑150 (MQ=255)
                                                                  ggAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTag                                                                                                                    <  1:1429144/114‑1 (MQ=255)
                                                                  ggAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTag                                                                                                                    >  2:1429144/1‑114 (MQ=255)
                                                                  ggAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGc                                                                                            <  2:601007/138‑1 (MQ=255)
                                                                  ggAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGc                                                                                            >  1:601007/1‑138 (MQ=255)
                                                                  ggAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGtt                                                                                <  2:1281465/150‑1 (MQ=255)
                                                                       cGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCaa                                                                           <  1:59839/150‑1 (MQ=255)
                                                                        gATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGaa                                                                                                                                    >  2:1153139/1‑92 (MQ=255)
                                                                        gATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGaa                                                                                                                                    <  1:1153139/92‑1 (MQ=255)
                                                                        gATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAg                                                                          >  1:979448/1‑150 (MQ=255)
                                                                          tCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGAc                                                                        >  1:1631794/1‑150 (MQ=255)
                                                                            agagAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCg                                                                      >  1:1833489/1‑150 (MQ=255)
                                                                            agagAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCg                                                                      >  1:1211277/1‑150 (MQ=255)
                                                                                aaTTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGc                                                                  <  2:1065573/150‑1 (MQ=255)
                                                                                aaTTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGc                                                                  >  1:102940/1‑150 (MQ=255)
                                                                                   tGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTACAATGTGGCGGAAGCGTAGAGATTCGAACTATAGGACAGTTGCCTATGGACGAGTTTCAAGACCGTTGCCtt                                                               >  1:821823/1‑150 (MQ=37)
                                                                                       gAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCg                                                                                                                             <  1:988911/84‑1 (MQ=255)
                                                                                       gAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCg                                                                                                                             >  2:988911/1‑84 (MQ=255)
                                                                                       gAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAAc                                                           <  2:1211277/150‑1 (MQ=255)
                                                                                       gAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAAc                                                           >  1:1314063/1‑150 (MQ=255)
                                                                                           ccaCCCGCATCACCGAAGCTATAAAACTAGAGTCTCAGAAACGCAAAAAGCCCTGAATAATCAGTGCTTTGAATATGGCGTAATCATATAGATACGAACTCTAGGATAGTTGCCCATCGACGATTTTCAAGACCGTTGCCTTAAACCACt                                                       <  2:504095/148‑1 (MQ=25)
                                                                                            gACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTGTCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTc                                                      >  1:1476045/1‑150 (MQ=255)
                                                                                                     gACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCg                                                                                                                             <  1:1668402/70‑1 (MQ=255)
                                                                                                     gACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCg                                                                                                                             >  2:1668402/1‑70 (MQ=255)
                                                                                                     aaCATAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTATGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCt                                             <  2:1171199/149‑1 (MQ=255)
                                                                                                      aCAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCtt                                            <  2:1242064/150‑1 (MQ=255)
                                                                                                        aGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGc                                                                  >  2:488668/1‑126 (MQ=255)
                                                                                                        aGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGc                                                                  <  1:488668/126‑1 (MQ=255)
                                                                                                          aaGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAg                                        >  2:1448270/1‑150 (MQ=255)
                                                                                                            aggAAAAAACTAGAGCCTCCGCTACGCCCCTTGGCCTTAATTGTCAGGGCTTTTAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCt                                      <  2:1476045/149‑1 (MQ=255)
                                                                                                              gAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGATTATTCAggg                                                                                                                                           <  1:20982/47‑1 (MQ=25)
                                                                                                              gAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGATTATTCAggg                                                                                                                                           >  2:20982/1‑47 (MQ=25)
                                                                                                                 aaaaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGt                                                                                 <  2:1531790/102‑1 (MQ=255)
                                                                                                                 aaaaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGt                                                                                 >  1:1531790/1‑102 (MQ=255)
                                                                                                                 aaaaCTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGttt                                 >  2:774270/1‑150 (MQ=255)
                                                                                                                          gCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGgccgc                        <  2:979448/150‑1 (MQ=255)
                                                                                                                            cTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCa                      <  1:831793/150‑1 (MQ=255)
                                                                                                                             tCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGAtt                                                                                                               >  2:338570/1‑60 (MQ=38)
                                                                                                                             tCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGAtt                                                                                                               <  1:338570/60‑1 (MQ=38)
                                                                                                                             tCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCat                     >  2:561855/1‑150 (MQ=255)
                                                                                                                                 aaaCGCAAAAAGCCCTGAATAATCAGGGCTTTGAACATGGCGGAAGCGTAGAGATTCGAACTCTAGGATATTTGCCCATCTACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCataata                 <  2:1037897/150‑1 (MQ=255)
                                                                                                                                   actcAAAAAGCCCTGAATAATAAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATAcc               <  2:933008/147‑1 (MQ=255)
                                                                                                                                   aCGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATAcc               >  2:520180/1‑150 (MQ=255)
                                                                                                                                   aCGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATAcc               <  2:821823/150‑1 (MQ=255)
                                                                                                                                       aaaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTaa           <  1:1413255/150‑1 (MQ=255)
                                                                                                                                       aaaaaGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTaa           <  1:561855/150‑1 (MQ=255)
                                                                                                                                           aGCCCTGATTATTCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCATTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGaa       >  2:285747/1‑150 (MQ=255)
                                                                                                                                            gCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGaaa      <  2:1314063/150‑1 (MQ=255)
                                                                                                                                            gCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGaaa      <  1:774270/150‑1 (MQ=255)
                                                                                                                                            gCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGaaa      >  1:896749/1‑150 (MQ=255)
                                                                                                                                               cTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAacac   >  2:981150/1‑150 (MQ=255)
                                                                                                                                                tGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACg  >  1:1805082/1‑150 (MQ=255)
                                                                                                                                                tGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACg  >  2:1479618/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAAAGCTCTAGGGGAAGCGCGTCACGCCAAGCTGAAGCAGCTCGCACACAGCATGTGTCGGATGCGGAAACGATCAGAGAATTGCGGAAACGACCCGGATGACAGAAGGGAAAAAACTAGAGCCTCAGAAACGCAAAAAGCCCTGAATAATCAGGGCTTTGAATATGGCGGAAGCGTAGAGATTCGAACTCTAGGATAGTTGCCCATCGACGGTTTTCAAGACCGTTGCCTTAAACCACTCGGCCACGCTTCCAGCTCGTTTTGCGGCCGCCATAATACCTTAATGAAACACG  >  NC_002947/4501194‑4501487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑