Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,522,283 T→G 5.3% F30V (TTC→GTC) ‡ idh → isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,522,2830TG 5.3% 226.7 / 3.8 76F30V (TTC→GTC) ‡idhisocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base G (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GATGCGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAA  >  NC_002947/4522134‑4522429
                                                                                                                                                     |                                                                                                                                                  
gATGCGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGt                                                                                                                                                    >  1:567320/1‑150 (MQ=255)
gATGCGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGt                                                                                                                                                    >  2:240970/1‑150 (MQ=255)
 aTGCGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGtt                                                                                                                                                   <  2:1068331/150‑1 (MQ=255)
  tGCGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGACGCGTTc                                                                                                                                                  >  2:624321/1‑150 (MQ=255)
    cgcCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCAc                                                                                                                                                >  1:1382834/1‑150 (MQ=255)
    cgcCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCAc                                                                                                                                                <  2:1803825/150‑1 (MQ=255)
     gcCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCAcc                                                                                                                                               >  2:620788/1‑150 (MQ=255)
     gcCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTAGCTGCTGCCGATCATCGAAGCGTTCAcc                                                                                                                                               >  1:1268669/1‑150 (MQ=255)
            cTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGGTGAACGCTTCga                                                                                                                                        <  2:967559/150‑2 (MQ=255)
                  accacGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACa                                                                                                                                  >  2:1551900/1‑150 (MQ=255)
                    cacGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCACCGATCATCTATACCTTCACCCGCTAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATc                                                                                                                                >  1:1205120/1‑150 (MQ=255)
                     acGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCg                                                                                                                               <  1:729779/150‑1 (MQ=255)
                      cGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGc                                                                                                                              >  1:1158844/1‑150 (MQ=255)
                       gagaCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCt                                                                                                                             <  1:1598651/150‑1 (MQ=255)
                               aGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAAc                                                                                                                     >  1:1086339/1‑150 (MQ=255)
                                  aCGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCGTCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGGTGAACGCTTCgat                                                                                                                                       <  1:749818/129‑3 (MQ=255)
                                  aCGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACTAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTcg                                                                                                                  >  1:276275/1‑150 (MQ=255)
                                  aCGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGGTGAACGCTTCgat                                                                                                                                       >  2:749818/1‑127 (MQ=255)
                                    gCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTcgcg                                                                                                                >  1:1518468/1‑150 (MQ=255)
                                         gCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACAtt                                                                                                           >  2:1824836/1‑150 (MQ=255)
                                           acacaGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTc                                                                                                         >  2:1258228/1‑150 (MQ=255)
                                             acaGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTccc                                                                                                       >  2:915512/1‑150 (MQ=255)
                                                gagagTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCctgg                                                                                                    <  2:1086339/150‑1 (MQ=255)
                                                  gagTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGACACTCGCGACATTTCCctggct                                                                                                  <  1:537054/150‑1 (MQ=255)
                                                  gagTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCctggct                                                                                                  <  2:1132329/150‑1 (MQ=255)
                                                    gTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCctggctgg                                                                                                <  1:1824836/150‑1 (MQ=255)
                                                     ttAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTccc                                                                                                       <  2:721144/142‑1 (MQ=255)
                                                     ttAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTccc                                                                                                       >  1:721144/1‑142 (MQ=255)
                                                     ttAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCtggctggc                                                                                               <  1:1772849/150‑1 (MQ=255)
                                                     ttAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCtggctggc                                                                                               >  2:1702749/1‑150 (MQ=255)
                                                      tAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGcc                                                                                              <  1:1702749/150‑1 (MQ=255)
                                                      tAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACCCGCTCCATATCCCTggccggcc                                                                                              <  2:567320/150‑1 (MQ=255)
                                                           cGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATc                                                                                         <  1:684794/150‑1 (MQ=255)
                                                              aTGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCTCTTCGGCTGACATCGCTGTCGAAACTAGCGACATTTCCCTGGCTGGCCGTATCCtt                                                                                      <  1:1551900/150‑1 (MQ=255)
                                                                gCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgc                                                                                    <  2:1056681/150‑1 (MQ=255)
                                                                      ccGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCtt                                                                              <  1:1434043/150‑1 (MQ=255)
                                                                        gTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTcc                                                                            <  1:1258228/150‑1 (MQ=255)
                                                                         ttCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTccc                                                                           <  2:1814711/150‑1 (MQ=255)
                                                                          tCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCg                                                                          <  1:1722695/150‑1 (MQ=255)
                                                                            cAAGATCATCTATACCTTCACCGACTAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                        <  2:276275/150‑1 (MQ=255)
                                                                            cAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                        <  1:517168/150‑1 (MQ=255)
                                                                            cAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                        <  1:240970/150‑1 (MQ=255)
                                                                              aGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGGCATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGc                                                                      <  2:1107174/150‑1 (MQ=255)
                                                                                 tcatcTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAAc                                                                   >  2:1540102/1‑150 (MQ=255)
                                                                                     cTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTggg                                                               <  1:624321/150‑1 (MQ=255)
                                                                                         aCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCa                                                           <  1:620788/150‑1 (MQ=255)
                                                                                          ccTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCag                                                          >  2:1831973/1‑150 (MQ=255)
                                                                                           cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCct                                                                                                      <  2:372802/105‑1 (MQ=255)
                                                                                           cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCct                                                                                                      >  1:372802/1‑105 (MQ=255)
                                                                                           cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCaga                                                         >  1:1704127/1‑150 (MQ=255)
                                                                                            ttCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCagag                                                        >  2:1756395/1‑150 (MQ=255)
                                                                                              cACCGACGAAGCCCCCGCCCTCGCCACCTCCTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTNGCTGGCCGTNTCCTTGCCNCCTTCCCGGAGCAACTGGGCGCAgagan                                                      <  1:743874/150‑2 (MQ=255)
                                                                                                 cgacgaAGCCCCCGCCCTCGCCACTTACTCGCTGCTTCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCa                                                   <  1:186023/150‑1 (MQ=255)
                                                                                                  aacgcggCCCGCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTTACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCaa                                                  >  2:965147/7‑150 (MQ=255)
                                                                                                      aaGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAg                                              <  1:915512/150‑1 (MQ=255)
                                                                                                       aGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAgg                                             <  2:322031/150‑1 (MQ=255)
                                                                                                          ccccGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCaga                                                         >  2:8731/1‑135 (MQ=255)
                                                                                                          ccccGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCaga                                                         <  1:8731/135‑1 (MQ=255)
                                                                                                                  tCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCAc                                      <  2:943129/146‑1 (MQ=255)
                                                                                                                  tCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCAc                                      >  1:943129/1‑146 (MQ=255)
                                                                                                                  tCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTgg                                  <  2:987992/150‑1 (MQ=255)
                                                                                                                    gCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCg                                >  2:90086/1‑150 (MQ=255)
                                                                                                                     ccACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCaa                                                                    <  1:971739/113‑1 (MQ=255)
                                                                                                                     ccACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCaa                                                                    >  2:971739/1‑113 (MQ=255)
                                                                                                                           aCTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTgg                         <  2:1518468/150‑1 (MQ=255)
                                                                                                                            cTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTggg                        >  1:490035/1‑150 (MQ=255)
                                                                                                                             tCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGc                       >  1:472065/1‑150 (MQ=255)
                                                                                                                               gctgctGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTgg                                                                <  2:1936956/107‑1 (MQ=255)
                                                                                                                               gcggctgcCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTgg                                                                >  1:1936956/4‑107 (MQ=255)
                                                                                                                                    tgcCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTgg                <  2:1917027/150‑1 (MQ=255)
                                                                                                                                        gATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTac            >  2:1579300/1‑150 (MQ=255)
                                                                                                                                        gATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTac            >  2:31468/1‑150 (MQ=255)
                                                                                                                                          cagccGAAGCGGTGAACGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTacca          >  1:1282368/5‑150 (MQ=255)
                                                                                                                                             tcGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTccc                                                                           >  2:744433/1‑82 (MQ=255)
                                                                                                                                             tcGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTccc                                                                           <  1:744433/82‑1 (MQ=255)
                                                                                                                                               gAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCt     <  1:31468/150‑1 (MQ=255)
                                                                                                                                               gAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCt     >  2:978782/1‑150 (MQ=255)
                                                                                                                                                 aGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGa   >  2:1569814/1‑150 (MQ=255)
                                                                                                                                                  gCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGaa  <  2:1704127/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GATGCGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAA  >  NC_002947/4522134‑4522429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑