Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,564,684	T→C	6.8%	intergenic (‑780/‑118)	vgrG‑III ← / → glgA	type VI secretion system protein/glycogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,564,684	0	T	C	6.8%	163.4 / 2.4	59	intergenic (‑780/‑118)	vgrG‑III/glgA	type VI secretion system protein/glycogen synthase
Reads supporting (aligned to +/- strand):  ref base T (24/31);  new base C (2/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

TCAATGGAAGAGTGACGAAAAAAAGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGC  >  NC_002947/4564535‑4564823                                                                                                                                                       |                                                                                                                                            tCAATGGAAGAGTGACGAAAAAAAGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCTGAACGCCGATC‑GCGCcgtgcgt                                                                                                                                             >  2:988651/1‑150 (MQ=255)        aaGAGTGACGAAAAAAAGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCa                                                                                                                                      >  1:418788/1‑150 (MQ=255)                  aaaaaaaGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAggg                                                                                                                            <  1:1786372/150‑1 (MQ=255)                       aaGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACATCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATg                                                                                                                       <  2:956901/150‑1 (MQ=255)                       aaGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCTCCGTGCGTGCGCGCACGACACAGGGGCATg                                                                                                                       <  1:374448/150‑1 (MQ=255)                            gAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACg                                                                                                                  >  1:1010212/1‑150 (MQ=255)                              aCTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GATCCGTGCGTGCGCGCACGACACAGGGCCATGGAACGCa                                                                                                                >  2:1423684/1‑150 (MQ=255)                                 ttACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTa                                                                                                             <  2:1690372/150‑1 (MQ=255)                                     ggAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACgca                                                                                                         <  1:1255144/150‑1 (MQ=255)                                     ggAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACgca                                                                                                         >  1:1510414/1‑150 (MQ=255)                                     ggAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACgca                                                                                                         >  1:596358/1‑150 (MQ=255)                                      gAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACgcag                                                                                                        <  1:87399/150‑1 (MQ=255)                                            gggCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCa                                                                                                  <  1:1243328/150‑1 (MQ=255)                                             ggCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCTGCCGGAACGCCGATC‑GCGTCGTGCGCGcacgcacg                                                                                                                                    >  2:52899/1‑115 (MQ=255)                                             ggCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCTGCCGGAACGCCGATC‑GCGTCGTGCGCGcacgcacg                                                                                                                                    <  1:52899/115‑1 (MQ=255)                                             ggCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGTCGTGCGCGcacgcacg                                                                                                                                    <  1:52919/115‑1 (MQ=255)                                             ggCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGTCGTGCGCGcacgcacg                                                                                                                                    >  2:52919/1‑115 (MQ=255)                                                aGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCagcaa                                                                                              <  2:959468/150‑1 (MQ=255)                                                  gTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCagcaagc                                                                                            >  1:102005/1‑150 (MQ=255)                                                   tCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCagcaagca                                                                                           >  2:890483/1‑150 (MQ=255)                                                   ccAGGATCTACGTAGTCTAAACGCAGAGTCACTTTTCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCagcaagca                                                                                           <  2:1214028/149‑1 (MQ=255)                                                          cTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACACACAGCGCAGCATTCAGCAAGCAGAGAGaa                                                                                    <  1:1507524/150‑1 (MQ=255)                                                                     aaaCGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGaaa                                                                         >  1:23361/1‑150 (MQ=255)                                                                       aCGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACa                                                                       >  2:1025601/1‑150 (MQ=255)                                                                        cGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACAt                                                                      <  1:116703/150‑1 (MQ=255)                                                                           aaaGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAgg                                                                                                                             <  1:1279339/92‑1 (MQ=255)                                                                           aaaGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAgg                                                                                                                             >  2:1279339/1‑92 (MQ=255)                                                                               ggACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCAc                                                                             >  1:1172715/1‑136 (MQ=255)                                                                               ggACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCAc                                                                             <  2:1172715/136‑1 (MQ=255)                                                                               ggACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACg                                                               <  1:1025601/150‑1 (MQ=255)                                                                                 aCTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGTGCTCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACggg                                                             >  2:188721/1‑150 (MQ=255)                                                                                       cAGCCATGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAg                                                       >  2:1686392/1‑150 (MQ=255)                                                                                       cAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATc                                                                     >  1:1516974/1‑136 (MQ=255)                                                                                       cAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATc                                                                     <  2:1516974/136‑1 (MQ=255)                                                                                               gCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCgtg                                               <  2:1510414/150‑3 (MQ=255)                                                                                                ccaccaACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGAcc                                              >  2:1709112/1‑150 (MQ=255)                                                                                                  accaACCCAACAGCCCAATCGCTTTCGGCC‑GAACTCCCTGCTGGCCCGTGCTTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa                                            <  1:433813/150‑1 (MQ=255)                                                                                                    caacccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGt                                                            >  1:195636/1‑132 (MQ=255)                                                                                                    caacccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGt                                                            <  2:195636/132‑1 (MQ=255)                                                                                                    caacccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGc                                          >  1:1796130/1‑150 (MQ=255)                                                                                                    caacccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGc                                          <  2:596358/150‑1 (MQ=255)                                                                                                    caacccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGc                                          <  2:1354975/150‑1 (MQ=255)                                                                                                        ccaacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAg                                      <  1:1715217/150‑1 (MQ=255)                                                                                                         caacaaCCCAATCGCTTCCGGCCGGAACGCCGAGC‑GCGCCGTGCGTGCGCCCACGACACAGGGGCATGGAACGCACCACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAgg                                     <  1:856264/150‑1 (MQ=255)                                                                                                         caacAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAgg                                     >  1:1244549/1‑150 (MQ=255)                                                                                                               cccAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAg                                                       <  1:744910/126‑1 (MQ=255)                                                                                                               cccAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAg                                                       >  2:744910/1‑126 (MQ=255)                                                                                                               cccAATCGCTTTCGGCCGGAACGCCAATC‑GCGCCGTGCCTGCGCCCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCACCACGAAACAGCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGga                               <  2:928615/150‑1 (MQ=255)                                                                                                                    tCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGaa                          <  2:23361/150‑1 (MQ=255)                                                                                                                     cGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAgg                                                                                                                             <  2:1607560/50‑1 (MQ=255)                                                                                                                     cGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAgg                                                                                                                             >  1:1607560/1‑50 (MQ=255)                                                                                                                             gCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGAGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACCGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCa                 >  2:536090/1‑150 (MQ=255)                                                                                                                                 gAACGCCGATC‑GCGCCGGCCGTGCGCGCACGACACAGGAGCATGGCACGCACTCCACACCCGTAAGTAAGCAGAGAGAAGTAGGACGAAACAACGCCACTGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGc             <  2:218619/150‑1 (MQ=255)                                                                                                                                     gCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCt         >  2:187559/1‑150 (MQ=255)                                                                                                                                     gCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGAACAGTGCCGcc         >  2:187565/1‑149 (MQ=255)                                                                                                                                             gcgcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGc  <  2:1796130/150‑1 (MQ=255)                                                                                                                                             gcgcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGc  <  1:890483/150‑1 (MQ=255)                                                                                                                                             gcgcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGAACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGc  <  2:1206962/150‑1 (MQ=255)                                                                                                                                               gcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATa                                       >  2:1279153/1‑111 (MQ=255)                                                                                                                                               gcCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATa                                       <  1:1279153/111‑1 (MQ=255)                                                                                                                                                tcgtgcgcgCACGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGc                                          >  2:1843495/2‑107 (MQ=255)                                                                                                                                                tcgtgcgcgCACGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGc                                          <  1:1843495/106‑1 (MQ=255)                                                                                                                                                   tgcgtgcgCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa                                            >  1:1235952/1‑102 (MQ=255)                                                                                                                                                   tgcgtgcgCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCaa                                            <  2:1235952/102‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                            TCAATGGAAGAGTGACGAAAAAAAGTCGAACTTTACGGAAAGCGGGCAGGTCAGGAACTAAGTAGGCCAAACGCAAAGGGACTTCCCAGCCACGGCCACCAACCCAACAGCCCAATCGCTTTCGGCCGGAACGCCGATC‑GCGCCGTGCGTGCGCGCACGACACAGGGGCATGGAACGCACTACGCAGCATTCAGCAAGCAGAGAGAACCAGCACGAAACATCGCCACGGGTGCCAGCACCCGACCAAGCATAGGGACGGAGAGAAGTATGATCAGTGCCGCTGTCGAGC  >  NC_002947/4564535‑4564823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑