Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,632,641	G→C	7.5%	noncoding (35/76 nt)	PP_t59 ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,632,641	0	G	C	7.5%	208.0 / 8.3	67	noncoding (35/76 nt)	PP_t59	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base C (3/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

GGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAG  >  NC_002947/4632499‑4632786                                                                                                                                               |                                                                                                                                                  gggCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTc                                                                                                                                            <  1:1834556/150‑1 (MQ=255)   gttgagTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGt                                                                                                                                          <  2:1077103/145‑1 (MQ=255)   gCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgg                                                                                                                                          <  2:92945/150‑2 (MQ=255)      gggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct                                                                                                                                       <  2:1145181/150‑1 (MQ=255)          aTTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                   <  2:780228/150‑1 (MQ=255)          aTTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                   <  2:749116/150‑1 (MQ=255)           ttGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAcc                                                                                                                                  >  1:825496/1‑150 (MQ=255)               ggCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                              <  1:1277295/150‑1 (MQ=255)                gCAGAATGCATTCGAACGAAAAAAGGCCCCGCAGGGCGTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCGTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                             >  1:155445/1‑150 (MQ=255)                gCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                             >  2:1786174/1‑150 (MQ=255)                 cAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGa                                                                                                                            <  2:825496/150‑1 (MQ=255)                   gAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGATCGGGAAACGGGGCTCGAATTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc                                                                                                                          <  1:1008792/150‑1 (MQ=255)                         aTTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                           >  1:1035041/1‑143 (MQ=255)                         aTTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                           <  2:1035041/143‑1 (MQ=255)                          ttCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                   >  1:1338739/1‑150 (MQ=255)                               acgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTGTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggggtc                                                                                                                                      <  2:641564/126‑6 (MQ=255)                               acgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTGTTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggggtc                                                                                                                                      >  1:641564/1‑121 (MQ=255)                               acgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtc                                                                                                              <  2:1338739/150‑1 (MQ=255)                                 gaaAAAAGGCCCTTCGGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtcgt                                                                                                            >  2:863568/1‑150 (MQ=255)                                 gaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                                                                                <  2:604159/130‑1 (MQ=255)                                 gaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                                                                                >  1:604159/1‑130 (MQ=255)                                    aaaaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGAt                                                                                                         >  1:554352/1‑150 (MQ=255)                                      aaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                       <  2:554352/150‑1 (MQ=255)                                      aaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                       >  1:646140/1‑150 (MQ=255)                                      aaGGCCCCGAAGGGCCTTTTTTCAACGAATTCCAGCATGCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                       <  2:646140/150‑1 (MQ=21)                                                 gggCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTa                                                                                            <  2:555463/150‑1 (MQ=255)                                                    ccTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCg                                                                                         >  2:1047179/1‑150 (MQ=255)                                                     cTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGa                                                                                        <  1:45460/150‑1 (MQ=255)                                                         tttCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGATCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTc                                                                                    >  1:1414574/1‑150 (MQ=255)                                                                   ttACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCg                                                                          >  1:1938467/1‑150 (MQ=255)                                                                   ttACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCg                                                                          >  1:698662/1‑150 (MQ=255)                                                                        gACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                           <  2:68246/96‑1 (MQ=255)                                                                        gACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                           >  1:68246/1‑96 (MQ=255)                                                                         aCTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAcc                                                                    >  2:1771104/1‑150 (MQ=255)                                                                           ttCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTAGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAcccc                                                                  >  2:758709/1‑150 (MQ=255)                                                                                cTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGa                                                              >  1:1862203/1‑149 (MQ=255)                                                                                cTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGa                                                              <  2:1862203/149‑1 (MQ=255)                                                                                    aaCCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCaa                                                         >  1:1526332/1‑150 (MQ=255)                                                                                       cTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCaaaaa                                                      >  2:88183/1‑150 (MQ=255)                                                                                        tGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTaaa                                                                                >  1:1294010/1‑123 (MQ=255)                                                                                        tGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTaaa                                                                                <  2:1294010/123‑1 (MQ=255)                                                                                          tAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                       <  1:623546/98‑1 (MQ=255)                                                                                          tAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATgg                                                                                                       >  2:623546/1‑98 (MQ=255)                                                                                             aTCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACtttt                                                >  1:291282/1‑150 (MQ=255)                                                                                              tCTTTATTTGTAGCGGGAAACGAGTCTCGAACTCCGGGCCCGGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTa                                               <  2:155445/150‑1 (MQ=37)                                                                                               cTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAAc                                                    >  2:129916/1‑144 (MQ=255)                                                                                               cTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAAc                                                    <  1:129916/144‑1 (MQ=255)                                                                                                tttATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCggg                                                                                                                                         >  2:221087/1‑56 (MQ=25)                                                                                                tttATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCggg                                                                                                                                         <  1:221087/58‑3 (MQ=25)                                                                                                tttATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTAtt                                             >  1:532007/1‑150 (MQ=255)                                                                                                          gCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGa                                   <  2:532007/150‑1 (MQ=255)                                                                                                            gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGc                                 <  2:1414574/150‑1 (MQ=255)                                                                                                                  cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTcgc                           <  1:863568/150‑1 (MQ=255)                                                                                                                    agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCGAAAAACTTTTATTTTTTTTCCGAGCCTTCgcgc                         <  2:1916098/150‑1 (MQ=255)                                                                                                                    agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCgcgc                         <  2:1598798/150‑1 (MQ=255)                                                                                                                        tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGa                                   >  2:5962/1‑136 (MQ=255)                                                                                                                        tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGa                                   <  1:5962/136‑1 (MQ=255)                                                                                                                        tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGt                     >  1:1170628/1‑150 (MQ=255)                                                                                                                              tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggcc               >  1:1004180/1‑150 (MQ=255)                                                                                                                              tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggcc               >  1:880206/1‑150 (MQ=255)                                                                                                                                   agcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcg          >  2:153544/5‑150 (MQ=255)                                                                                                                                    gcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcgg         >  1:1404226/4‑150 (MQ=255)                                                                                                                                     cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggc        <  2:698662/150‑1 (MQ=255)                                                                                                                                      ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggcc       >  1:1799382/1‑150 (MQ=255)                                                                                                                                       cGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcggcca      >  1:539821/1‑150 (MQ=255)                                                                                                                                           cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCcagcag  >  2:1837341/1‑150 (MQ=255)                                                                                                                                           cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCcagcag  <  1:1047179/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  GGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAG  >  NC_002947/4632499‑4632786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑