Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 296,370 C→A 7.5% A20S (GCG→TCG)  PP_0242 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947296,3700CA 7.5% 336.8 / 11.7 106A20S (GCG→TCG) PP_0242TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (51/47);  new base A (4/4);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 108 total aligned reads displayed.

TGCAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGC  >  NC_002947/296221‑296514
                                                                                                                                                     |                                                                                                                                                
tGCAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGc                                                                                                                                                  >  1:1072689/1‑150 (MQ=255)
 gCAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGcc                                                                                                                                                 >  2:288179/1‑150 (MQ=255)
  cAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCt                                                                                                                                                >  2:746784/1‑150 (MQ=255)
          gCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGCAAGTGCTGCCGCCCTGTAGTTGGCTAGCAGCTCACGCGCAGCCGCCTGGATCGCCTCGATcctg                                                                                                                                        >  2:1389870/1‑150 (MQ=255)
           ccTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctg                                                                                                                                        >  2:687402/1‑149 (MQ=255)
           ccTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctg                                                                                                                                        <  1:687402/149‑1 (MQ=255)
            cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggc                                                                                                                                      >  2:1648863/1‑150 (MQ=255)
            cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggc                                                                                                                                      <  1:1389870/150‑1 (MQ=255)
             tCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcc                                                                                                                                     >  2:1398509/1‑150 (MQ=255)
               cAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACCCCGACGAAAGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcctg                                                                                                                                   >  2:559515/1‑150 (MQ=255)
                aGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggatcag                                                                                                                                  <  1:559515/150‑6 (MQ=255)
                    aGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGgctgc                                                                                                                              <  1:1648863/150‑1 (MQ=255)
                        cGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgc                                                                                                                          >  2:1149961/1‑150 (MQ=255)
                          gCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgcgc                                                                                                                        <  1:1043061/150‑1 (MQ=255)
                             cGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTg                                                                                                                     <  2:1669137/150‑1 (MQ=255)
                                    gAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGtt                                                                                                                  <  2:1295541/146‑1 (MQ=255)
                                    gAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGtt                                                                                                                  >  1:1295541/1‑146 (MQ=255)
                                     aaaaaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGTCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTgcgc                                                                                                             >  2:1406485/1‑150 (MQ=255)
                                       aaaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCgg                                                                                                           >  1:1564103/1‑150 (MQ=255)
                                        aaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCggg                                                                                                          <  1:324607/150‑1 (MQ=255)
                                             aTACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggt                                                                                                     <  2:1124353/150‑1 (MQ=255)
                                              tACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                    <  1:746784/150‑1 (MQ=255)
                                              tACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtgctg                                                                                                    <  1:1936479/150‑1 (MQ=255)
                                                 acCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGa                                                                                                                                             <  1:1384660/106‑1 (MQ=255)
                                                  ccGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggc                                                                                                                                      <  1:1027687/112‑1 (MQ=255)
                                                  ccGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggc                                                                                                                                      >  2:1027687/1‑112 (MQ=255)
                                                   cgacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                               >  1:231707/1‑150 (MQ=255)
                                                    gacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCgg                                                                                              <  2:1072689/150‑1 (MQ=255)
                                                     acgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGt                                                                                             >  2:537416/1‑150 (MQ=255)
                                                     acgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGt                                                                                             <  2:231707/150‑1 (MQ=255)
                                                          ggCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTTCGGTtcatc                                                                                        <  2:4342/150‑1 (MQ=255)
                                                           gCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtcatca                                                                                       >  1:476011/1‑150 (MQ=255)
                                                           gCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtcatca                                                                                       >  2:1183339/1‑150 (MQ=255)
                                                            cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCTCGTGGTATCAGCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                      >  1:1304611/1‑150 (MQ=255)
                                                            cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                      <  2:1143684/150‑1 (MQ=255)
                                                            cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                      <  2:476011/150‑1 (MQ=255)
                                                                 tCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAActgct                                                                                 >  1:1750640/1‑150 (MQ=255)
                                                                      gcCCGCTCGGCCACGCTGCAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGCGGTGGTGGTGCGGTTCATCAACTGCTGATTg                                                                            >  2:646414/1‑150 (MQ=255)
                                                                       gccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGc                                                                           >  1:1524138/2‑150 (MQ=255)
                                                                        ccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGcc                                                                          >  2:1723635/1‑150 (MQ=255)
                                                                         cGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCACCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                         <  1:425627/150‑1 (MQ=255)
                                                                          gCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAg                                                                        >  1:1126167/1‑150 (MQ=255)
                                                                                ccACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCt                                                                  <  2:560842/150‑1 (MQ=255)
                                                                                   cGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGc                                                               <  1:652313/150‑1 (MQ=255)
                                                                                     cTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCt                                                             <  1:288179/150‑1 (MQ=255)
                                                                                        tagatagaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGt                                                          <  2:1564103/150‑1 (MQ=255)
                                                                                          gatagaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAg                                                        >  2:1344583/1‑150 (MQ=255)
                                                                                             agaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGgcg                                                     >  2:1306907/1‑150 (MQ=255)
                                                                                              gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                    <  2:922007/86‑1 (MQ=255)
                                                                                              gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                    >  1:922007/1‑86 (MQ=255)
                                                                                              gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGgcgc                                                    <  1:537416/150‑1 (MQ=255)
                                                                                               aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                    >  1:739451/1‑101 (MQ=255)
                                                                                               aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                    <  2:739451/101‑1 (MQ=255)
                                                                                               aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGcc                                                   >  2:161560/1‑150 (MQ=255)
                                                                                               aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGcc                                                   >  1:1866243/1‑150 (MQ=255)
                                                                                                       gcCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACa                                           >  1:1814740/1‑150 (MQ=255)
                                                                                                        ccACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCgg                                                                                                           >  1:1265308/1‑85 (MQ=255)
                                                                                                        ccACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCgg                                                                                                           <  2:1265308/85‑1 (MQ=255)
                                                                                                          aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGAGCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGGTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGt                                        >  1:1851093/1‑150 (MQ=255)
                                                                                                            cccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCg                                      >  1:88529/1‑150 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGa                                                                               >  2:1630336/1‑107 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGa                                                                               <  1:1630336/107‑1 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACa                                           >  1:1614724/1‑143 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACa                                           <  2:1614724/143‑1 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                    <  2:667566/150‑1 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                    >  2:1272161/1‑150 (MQ=255)
                                                                                                              cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                    >  1:771789/1‑150 (MQ=255)
                                                                                                                  ttCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCa                                <  1:1183339/150‑1 (MQ=255)
                                                                                                                   tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                               <  1:1149961/150‑1 (MQ=255)
                                                                                                                   tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                               <  2:88529/150‑1 (MQ=255)
                                                                                                                   tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                               <  2:1814740/150‑1 (MQ=255)
                                                                                                                   tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                               <  1:161560/150‑1 (MQ=255)
                                                                                                                    cGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAcc                              <  2:771789/150‑1 (MQ=255)
                                                                                                                      gCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGATGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATTCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGa                            >  2:305571/1‑150 (MQ=255)
                                                                                                                        cAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGGCGAAGTCACCGACt                          >  2:724638/1‑150 (MQ=255)
                                                                                                                        cAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCCGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACt                          >  1:782194/1‑150 (MQ=255)
                                                                                                                          aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTgg                                                           >  1:789286/1‑115 (MQ=255)
                                                                                                                          aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTgg                                                           <  2:789286/115‑1 (MQ=255)
                                                                                                                          aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCACATAGGCGCCGACGAACAGGTCGAAGTCACCGACTTc                        <  1:646414/150‑1 (MQ=255)
                                                                                                                               tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAAc                                            >  1:861384/1‑125 (MQ=255)
                                                                                                                               tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAAc                                            <  2:861384/125‑1 (MQ=255)
                                                                                                                               tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGtttt                   >  2:432588/1‑150 (MQ=255)
                                                                                                                               tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGtttt                   >  2:1932440/1‑150 (MQ=255)
                                                                                                                                 aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGTTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                    <  1:1496801/131‑1 (MQ=255)
                                                                                                                                 aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGTTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                    >  2:1496801/1‑131 (MQ=255)
                                                                                                                                 aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGc                 >  1:765951/1‑150 (MQ=255)
                                                                                                                                       aGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCAGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGc           >  1:1586666/1‑150 (MQ=255)
                                                                                                                                         aggccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtca                                                                                          >  2:515673/3‑69 (MQ=25)
                                                                                                                                         aggccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtca                                                                                          <  1:515673/67‑1 (MQ=25)
                                                                                                                                          cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGCTTGCCAGGATGCTGGGGTGGCACGCTGGCACGAACAGGTGGAGGTCACCGACTTCGTTTTGCTCCAGCTCg        <  1:1306907/150‑1 (MQ=255)
                                                                                                                                          cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGACCAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCg        <  2:1304611/150‑1 (MQ=255)
                                                                                                                                          cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCCGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCg        <  2:782194/150‑1 (MQ=255)
                                                                                                                                           gccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTg                                                            <  1:1180319/97‑1 (MQ=255)
                                                                                                                                           gccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTg                                                            >  2:1180319/1‑97 (MQ=255)
                                                                                                                                            ccAGGATCGCCTCGATCCTGGCCTGGATGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGa      >  1:1647165/1‑150 (MQ=255)
                                                                                                                                                gATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc  <  1:69349/150‑1 (MQ=255)
                                                                                                                                                  tCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc  <  2:783849/148‑1 (MQ=255)
                                                                                                                                                  tCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc  >  1:783849/1‑148 (MQ=255)
                                                                                                                                                    gCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                         <  2:1916292/75‑1 (MQ=255)
                                                                                                                                                    gCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                         >  1:1916292/1‑75 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TGCAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGC  >  NC_002947/296221‑296514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑