Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 296,372 T→G 7.5% E19A (GAG→GCG) ‡ PP_0242 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947296,3720TG 7.5% 305.4 / 12.9 105E19A (GAG→GCG) ‡PP_0242TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (50/47);  new base G (4/4);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGGCGA  >  NC_002947/296223‑296521
                                                                                                                                                     |                                                                                                                                                     
cAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCt                                                                                                                                                       >  2:746784/1‑150 (MQ=255)
        gCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGCAAGTGCTGCCGCCCTGTAGTTGGCTAGCAGCTCACGCGCAGCCGCCTGGATCGCCTCGATcctg                                                                                                                                               >  2:1389870/1‑150 (MQ=255)
         ccTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctg                                                                                                                                               >  2:687402/1‑149 (MQ=255)
         ccTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctg                                                                                                                                               <  1:687402/149‑1 (MQ=255)
          cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggc                                                                                                                                             >  2:1648863/1‑150 (MQ=255)
          cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggc                                                                                                                                             <  1:1389870/150‑1 (MQ=255)
          cTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGAGCGCCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggc                                                                                                                                             >  2:652313/1‑150 (MQ=255)
           tCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcc                                                                                                                                            >  2:1398509/1‑150 (MQ=255)
             cAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACCCCGACGAAAGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcctg                                                                                                                                          >  2:559515/1‑150 (MQ=255)
              aGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggatcag                                                                                                                                         <  1:559515/150‑6 (MQ=255)
                  aGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGgctgc                                                                                                                                     >  1:1862045/1‑150 (MQ=255)
                  aGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGgctgc                                                                                                                                     <  1:1648863/150‑1 (MQ=255)
                      cGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgc                                                                                                                                 >  2:1149961/1‑150 (MQ=255)
                        gCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgcgc                                                                                                                               <  1:1043061/150‑1 (MQ=255)
                                  gAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGtt                                                                                                                         <  2:1295541/146‑1 (MQ=255)
                                  gAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGtt                                                                                                                         >  1:1295541/1‑146 (MQ=255)
                                   aaaaaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGTCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTgcgc                                                                                                                    >  2:1406485/1‑150 (MQ=255)
                                      aaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCggg                                                                                                                 <  1:324607/150‑1 (MQ=255)
                                           aTACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggt                                                                                                            <  2:1124353/150‑1 (MQ=255)
                                            tACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                           <  1:746784/150‑1 (MQ=255)
                                            tACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtgctg                                                                                                           <  1:1936479/150‑1 (MQ=255)
                                               acCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGa                                                                                                                                                    >  2:1384660/1‑106 (MQ=255)
                                               acCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGa                                                                                                                                                    <  1:1384660/106‑1 (MQ=255)
                                               acCGACGAAGGCGGAATCTGCGCCCGCTCGGACAAGCTGTAGAGATAAGGTTCTGCCACCCCTTGTTCGGCTGAGAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGgtggtggtg                                                                                                        <  1:1398509/150‑1 (MQ=255)
                                                ccGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggc                                                                                                                                             <  1:1027687/112‑1 (MQ=255)
                                                ccGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggc                                                                                                                                             >  2:1027687/1‑112 (MQ=255)
                                                 cgacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                      >  1:231707/1‑150 (MQ=255)
                                                  gacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCgg                                                                                                     <  2:1072689/150‑1 (MQ=255)
                                                   acgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGt                                                                                                    <  2:231707/150‑1 (MQ=255)
                                                   acgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGt                                                                                                    >  2:537416/1‑150 (MQ=255)
                                                        ggCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTTCGGTtcatc                                                                                               <  2:4342/150‑1 (MQ=255)
                                                         gCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtcatca                                                                                              >  2:1183339/1‑150 (MQ=255)
                                                         gCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtcatca                                                                                              >  1:476011/1‑150 (MQ=255)
                                                          cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCTCGTGGTATCAGCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                             >  1:1304611/1‑150 (MQ=255)
                                                          cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                             <  2:1143684/150‑1 (MQ=255)
                                                          cGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCaa                                                                                             <  2:476011/150‑1 (MQ=255)
                                                               tCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAActgct                                                                                        >  1:1750640/1‑150 (MQ=255)
                                                                 tGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGa                                                                                      <  2:1862045/150‑1 (MQ=255)
                                                                     gccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGc                                                                                  >  1:1524138/2‑150 (MQ=255)
                                                                      ccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGcc                                                                                 >  2:1723635/1‑150 (MQ=255)
                                                                       cGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCACCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                                <  1:425627/150‑1 (MQ=255)
                                                                        gCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAg                                                                               >  1:1126167/1‑150 (MQ=255)
                                                                              ccACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCt                                                                         <  2:560842/150‑1 (MQ=255)
                                                                              ccACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctgg                                                                                                                                              >  1:965027/1‑81 (MQ=38)
                                                                               cACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTg                                                                        >  1:667566/1‑150 (MQ=255)
                                                                                 cGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGc                                                                      <  1:652313/150‑1 (MQ=255)
                                                                                      tagatagaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGt                                                                 <  2:1564103/150‑1 (MQ=255)
                                                                                        gatagaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAg                                                               >  2:1344583/1‑150 (MQ=255)
                                                                                           agaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGgcg                                                            >  2:1306907/1‑150 (MQ=255)
                                                                                            gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                           >  1:922007/1‑86 (MQ=255)
                                                                                            gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTgg                                                                                                                           <  2:922007/86‑1 (MQ=255)
                                                                                            gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGgcgc                                                           <  1:537416/150‑1 (MQ=255)
                                                                                             aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                           >  1:739451/1‑101 (MQ=255)
                                                                                             aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                           <  2:739451/101‑1 (MQ=255)
                                                                                             aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGcc                                                          >  2:161560/1‑150 (MQ=255)
                                                                                             aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGcc                                                          >  1:1866243/1‑150 (MQ=255)
                                                                                                     gcCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACa                                                  >  1:1814740/1‑150 (MQ=255)
                                                                                                      ccACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCgg                                                                                                                  <  2:1265308/85‑1 (MQ=255)
                                                                                                      ccACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCgg                                                                                                                  >  1:1265308/1‑85 (MQ=255)
                                                                                                        aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGAGCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGGTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGt                                               >  1:1851093/1‑150 (MQ=255)
                                                                                                          cccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCg                                             >  1:88529/1‑150 (MQ=255)
                                                                                                            cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGa                                                                                      >  2:1630336/1‑107 (MQ=255)
                                                                                                            cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGa                                                                                      <  1:1630336/107‑1 (MQ=255)
                                                                                                            cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACa                                                  >  1:1614724/1‑143 (MQ=255)
                                                                                                            cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACa                                                  <  2:1614724/143‑1 (MQ=255)
                                                                                                            cTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                           <  2:667566/150‑1 (MQ=255)
                                                                                                                ttCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCa                                       <  1:1183339/150‑1 (MQ=255)
                                                                                                                 tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                                      <  2:1814740/150‑1 (MQ=255)
                                                                                                                 tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                                      <  1:1149961/150‑1 (MQ=255)
                                                                                                                 tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                                      <  1:161560/150‑1 (MQ=255)
                                                                                                                 tCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAc                                      <  2:88529/150‑1 (MQ=255)
                                                                                                                  cGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCAcc                                     <  2:771789/150‑1 (MQ=255)
                                                                                                                    gCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGATGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATTCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGa                                   >  2:305571/1‑150 (MQ=255)
                                                                                                                      cAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGGCGAAGTCACCGACt                                 >  2:724638/1‑150 (MQ=255)
                                                                                                                      cAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCCGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACt                                 >  1:782194/1‑150 (MQ=255)
                                                                                                                        aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTgg                                                                  <  2:789286/115‑1 (MQ=255)
                                                                                                                        aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTgg                                                                  >  1:789286/1‑115 (MQ=255)
                                                                                                                        aCAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCACATAGGCGCCGACGAACAGGTCGAAGTCACCGACTTc                               <  1:646414/150‑1 (MQ=255)
                                                                                                                             tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAAc                                                   <  2:861384/125‑1 (MQ=255)
                                                                                                                             tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAAc                                                   >  1:861384/1‑125 (MQ=255)
                                                                                                                             tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGtttt                          >  2:1932440/1‑150 (MQ=255)
                                                                                                                             tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGtttt                          >  2:432588/1‑150 (MQ=255)
                                                                                                                               aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGTTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                           <  1:1496801/131‑1 (MQ=255)
                                                                                                                               aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGTTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGaa                                           >  2:1496801/1‑131 (MQ=255)
                                                                                                                               aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGc                        >  1:765951/1‑150 (MQ=255)
                                                                                                                                     aGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCAGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGc                  >  1:1586666/1‑150 (MQ=255)
                                                                                                                                       aggccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtca                                                                                                 >  2:515673/3‑69 (MQ=25)
                                                                                                                                       aggccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtca                                                                                                 <  1:515673/67‑1 (MQ=25)
                                                                                                                                        cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGCTTGCCAGGATGCTGGGGTGGCACGCTGGCACGAACAGGTGGAGGTCACCGACTTCGTTTTGCTCCAGCTCg               <  1:1306907/150‑1 (MQ=255)
                                                                                                                                        cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGACCAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCg               <  2:1304611/150‑1 (MQ=255)
                                                                                                                                        cgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCCGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCg               <  2:782194/150‑1 (MQ=255)
                                                                                                                                         gccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTg                                                                   <  1:1180319/97‑1 (MQ=255)
                                                                                                                                         gccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTg                                                                   >  2:1180319/1‑97 (MQ=255)
                                                                                                                                          ccAGGATCGCCTCGATCCTGGCCTGGATGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGa             >  1:1647165/1‑150 (MQ=255)
                                                                                                                                              gATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc         <  1:69349/150‑1 (MQ=255)
                                                                                                                                                tCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc         >  1:783849/1‑148 (MQ=255)
                                                                                                                                                tCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGc         <  2:783849/148‑1 (MQ=255)
                                                                                                                                                  gCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                                <  2:1916292/75‑1 (MQ=255)
                                                                                                                                                  gCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCa                                                                                >  1:1916292/1‑75 (MQ=255)
                                                                                                                                                     tCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGgcga  <  2:765951/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGTAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGGCGA  >  NC_002947/296223‑296521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑