Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,937,770 C→A 10.0% G343V (GGG→GTG)  ddlA ← D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,937,7700CA10.0% 116.8 / 3.4 40G343V (GGG→GTG) ddlAD‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base A (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

TTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCTCTTCTGTCAAGAAAAAATCC  >  NC_002947/4937622‑4937916
                                                                                                                                                     |                                                                                                                                                  
ttGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGccc                                                                                                                                                   <  2:279766/150‑1 (MQ=255)
         tGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAA‑TCTTAGCGCGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGAc                                                                                                                                          <  1:101697/150‑1 (MQ=255)
             ccAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGA‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAgc                                                                                                                                      <  1:209470/150‑1 (MQ=255)
               aaGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCTGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAgcgc                                                                                                                                    >  2:23707/1‑150 (MQ=255)
                 gggTGATCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTc                                                                                                                                  <  1:569568/150‑1 (MQ=255)
                  ggTGATCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTcc                                                                                                                                 >  2:1463360/1‑150 (MQ=255)
                       tCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCACATATTCGACTAGCGCTCCTACCa                                                                                                                            >  2:971515/1‑150 (MQ=255)
                             aGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTcg                                                                                                                      >  2:816384/1‑150 (MQ=255)
                             aGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTcg                                                                                                                      >  2:1328529/1‑150 (MQ=255)
                                  ccAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCTGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGa                                                                                                                 <  1:23707/150‑1 (MQ=255)
                                                gC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCa                                                                                                   <  1:1328529/150‑1 (MQ=255)
                                                gC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATGCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACTGGAGCa                                                                                                   <  2:1407573/150‑1 (MQ=255)
                                                       cACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCaaa                                                                                             <  1:1845580/150‑1 (MQ=255)
                                                        aCCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTa                                                                                                                                        >  2:308621/1‑106 (MQ=255)
                                                        aCCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTa                                                                                                                                        <  1:308621/106‑1 (MQ=255)
                                                          cTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGAAGCACTCAAACac                                                                                          >  2:859558/1‑149 (MQ=255)
                                                             gCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCtt                                                                                       <  1:1797726/150‑1 (MQ=255)
                                                             gCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCATACCAACGGCGGCGGAGGGTAACCGGAGCACTCAAACATCtt                                                                                       <  1:859558/150‑1 (MQ=255)
                                                               cGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCg                                                                                     >  2:1244052/1‑150 (MQ=255)
                                                                   ggACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTa                                                                                 <  1:816384/150‑1 (MQ=255)
                                                                           tGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCACATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTgg                                                                         <  1:971515/150‑1 (MQ=255)
                                                                                       gTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTc                                                                                                                       >  2:247356/1‑92 (MQ=255)
                                                                                       gTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTc                                                                                                                       <  1:247356/92‑1 (MQ=255)
                                                                                          tCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCgg                                                          <  1:1463360/150‑1 (MQ=255)
                                                                                             tGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTa                                                       >  2:265743/1‑150 (MQ=255)
                                                                                              gagaAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCAGGGTaa                                                      >  1:281926/1‑150 (MQ=255)
                                                                                                             cAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCAtt                                       <  1:1244052/150‑1 (MQ=255)
                                                                                                                      tCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGaataat                              >  2:1105634/1‑150 (MQ=255)
                                                                                                                      tCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGaataat                              <  1:265743/150‑1 (MQ=255)
                                                                                                                      tCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGaataat                              >  2:1572096/1‑150 (MQ=255)
                                                                                                                        aTAAACGTTCTTTAGATGCTGATGAGAGCACATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTc                                                                                      >  1:1115541/1‑92 (MQ=255)
                                                                                                                        aTAAACGTTCTTTAGATGCTGATGAGAGCACATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTc                                                                                      <  2:1115541/92‑1 (MQ=255)
                                                                                                                         tAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTc                           >  2:1255801/1‑150 (MQ=255)
                                                                                                                           aaCGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCAc                         >  1:1460071/1‑150 (MQ=255)
                                                                                                                            aCGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCa                                                                       <  2:1229703/103‑1 (MQ=255)
                                                                                                                            aCGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCa                                                                       >  1:1229703/1‑103 (MQ=255)
                                                                                                                                   ttAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCtcttct                 >  1:1214661/1‑150 (MQ=255)
                                                                                                                                        tGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCTCTTCTGTCaa            >  1:658834/1‑150 (MQ=255)
                                                                                                                                             atgaGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTAAACGGTGCCTCTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCTCTTCTGTCAAGaaaa       >  1:1304420/1‑150 (MQ=255)
                                                                                                                                                agagCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCTCTTCTGTCAAGAAAAAAt    <  2:1460071/150‑1 (MQ=255)
                                                                                                                                                  agCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCTCTTCTGTCAAGAAAAAATcc  >  2:82778/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTGAGCGCTTGCGCCAAGGGTGATCGCGAAGCCGCCAAGCAATTTTTAGC‑CGAGCACCTTGCCGAGGGACGAGATGCAATCGTTACGTTTCTTGAGAAGCGGCGTCGGCAGGACGTGTCATAAACGTTCTTTAGATGCTGATGAGAGCCCATATTCGACTAGCGCTCCTACCAACTTCGGCGGAGGGTAACCGGAGCACTCAAACATCTTCGGGTACATACTGGCACTCGTAAATCCGGGTAAGGTATTGATCTCATTGAGAATAATTTCACCCTCTTCTGTCAAGAAAAAATCC  >  NC_002947/4937622‑4937916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑