Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,155,372 G→A 6.0% A57V (GCG→GTG)  PP_5698 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,155,3720GA 6.0% 200.0 / 2.2 67A57V (GCG→GTG) PP_5698hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base A (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

ATTCCGCGCTGCAGTTCAAGGCGATCCGCAGTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAGGGGCAGGCACATCAGTATT  >  NC_002947/5155229‑5155521
                                                                                                                                               |                                                                                                                                                     
aTTCCGCGCTGCAGTTCAAGGCGATCCGCAGTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGccgccg                                                                                                                                                 >  2:1886483/1‑150 (MQ=255)
           cAGTTCAAGGCGATCCGCAGTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGGGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCa                                                                                                                                      >  1:1631246/1‑150 (MQ=255)
           cAGTTCAAGGCGATCCGCAGTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCa                                                                                                                                      >  1:1618064/1‑150 (MQ=255)
              ttCAAGGCGATCCGCAGTTTGATGATTTGCCGCTTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAg                                                                                                                                   >  1:780351/1‑150 (MQ=255)
                     cGATCCGCAGTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGa                                                                                                                            <  2:1235065/150‑1 (MQ=255)
                              gTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATgg                                                                                                                   >  1:1396484/1‑150 (MQ=255)
                                 tgatgaTTTGCCGATTGAATTTCGAGGTGCCATCGCCGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGgcgc                                                                                                                <  2:1286384/150‑1 (MQ=255)
                                           ccGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATa                                                                                                      <  2:1365696/150‑1 (MQ=255)
                                             gATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAgttgtt                                                                                                                              >  1:812086/1‑124 (MQ=255)
                                             gATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAgttgtt                                                                                                                              <  2:812086/124‑1 (MQ=255)
                                                    tttCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTc                                                                                             >  1:966295/1‑150 (MQ=255)
                                                         cgGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAgg                                                                                        <  1:1663541/150‑1 (MQ=255)
                                                           gTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGACTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCg                                                                                      <  1:1812013/150‑1 (MQ=255)
                                                                aTCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTa                                                                                 >  2:1901352/1‑150 (MQ=255)
                                                                 tCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGGGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAg                                                                                <  2:1631246/150‑1 (MQ=255)
                                                                   gACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTc                                                                              >  1:637751/1‑150 (MQ=255)
                                                                        ttttACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGgt                                                                                    >  2:1774440/1‑139 (MQ=255)
                                                                        ttttACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGgt                                                                                    <  1:1774440/139‑1 (MQ=255)
                                                                        ttttACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCt                                                                         >  1:1083440/1‑150 (MQ=255)
                                                                         tttACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGcat                                                                        <  1:68283/150‑3 (MQ=255)
                                                                         tttACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCtt                                                                        <  2:780351/150‑1 (MQ=255)
                                                                             cccGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCTAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGc                                                                          <  1:1568155/144‑1 (MQ=255)
                                                                             cccGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGc                                                                          >  2:1568155/1‑144 (MQ=255)
                                                                              ccgccgGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACACCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGa                                                                            <  2:1119898/141‑1 (MQ=255)
                                                                              ccgccgGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACACCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGa                                                                            >  1:1119898/1‑141 (MQ=255)
                                                                               cgccgGTACGTTCACCCGGCGCACCCGCGGTGCCTATGGCAGACGTGTGATCTTCAGCTTTCACGCCGCCGGCCAATGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGAcccc                                                                  <  1:1886483/150‑1 (MQ=255)
                                                                                      aCGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGc                                                           >  2:273562/1‑150 (MQ=255)
                                                                                      aCGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATGACAGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGc                                                           <  1:1469934/150‑1 (MQ=255)
                                                                                        gTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCCCGCAGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTc                                                         >  2:752261/1‑150 (MQ=255)
                                                                                        gTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCg                                                                                      <  2:68491/121‑1 (MQ=255)
                                                                                        gTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCg                                                                                      >  1:68491/1‑121 (MQ=255)
                                                                                        gTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTc                                                         <  2:1396484/150‑1 (MQ=255)
                                                                                         ttCACCCGGCGCACCCGCGGTGCCTCTGGTCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCGCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTcc                                                        >  1:1323014/1‑150 (MQ=255)
                                                                                         ttCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTcc                                                        >  2:532772/1‑150 (MQ=255)
                                                                                            aCCCGGCGCACTCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAg                                                                                                                                   <  1:1575147/72‑1 (MQ=255)
                                                                                             cccGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGcc                                                                                                  <  1:1203640/104‑1 (MQ=255)
                                                                                             cccGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGcc                                                                                                  >  2:1203640/1‑104 (MQ=255)
                                                                                             cccGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTtct                                                    <  2:995441/150‑1 (MQ=255)
                                                                                              ccGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTtctc                                                   <  1:499061/150‑1 (MQ=255)
                                                                                              ccGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTtc                                                     >  1:1049963/1‑148 (MQ=255)
                                                                                              ccGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTtc                                                     <  2:1049963/148‑1 (MQ=255)
                                                                                                ggCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCca                                                 >  1:446746/1‑150 (MQ=255)
                                                                                                 gcgcACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCcaa                                                >  1:988561/1‑149 (MQ=255)
                                                                                                    cACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAgg                                                                                        <  2:398714/107‑1 (MQ=255)
                                                                                                    cACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAgg                                                                                        >  1:398714/1‑107 (MQ=255)
                                                                                                     aCCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGc                                                                                       <  2:1734749/107‑1 (MQ=255)
                                                                                                     aCCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGc                                                                                       >  1:1734749/1‑107 (MQ=255)
                                                                                                       ccGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACACCGCCGGGACCTGCCCATAgttgtt                                                                                                                              >  1:1196515/1‑66 (MQ=255)
                                                                                                       ccGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACACCGCCGGGACCTGCCCATAgttgtt                                                                                                                              <  2:1196515/66‑1 (MQ=255)
                                                                                                           ggTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGa                                      <  2:1618064/150‑1 (MQ=255)
                                                                                                               ccTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGCCCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCg                                                                 >  1:930865/1‑119 (MQ=255)
                                                                                                               ccTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCg                                                                 <  2:930865/119‑1 (MQ=255)
                                                                                                               ccTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAg                                  <  1:273562/150‑1 (MQ=255)
                                                                                                                 tctGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTTGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGcc                                >  2:1822244/1‑150 (MQ=255)
                                                                                                                 tctGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTcc                                                  >  1:1148696/1‑132 (MQ=255)
                                                                                                                 tctGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTcc                                                  <  2:1148696/132‑1 (MQ=255)
                                                                                                                 tctGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGcc                                >  2:340292/1‑150 (MQ=255)
                                                                                                                  ctGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCa                               <  1:340292/150‑1 (MQ=255)
                                                                                                                      gCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGc                           >  2:850842/1‑150 (MQ=255)
                                                                                                                          ttACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCg                       >  2:1779114/1‑150 (MQ=255)
                                                                                                                              ggAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGCGGTCGCTGGCCGCCGACTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAggg                   >  2:1506509/1‑150 (MQ=255)
                                                                                                                                aaGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAGGggc                 >  2:1128237/1‑150 (MQ=255)
                                                                                                                                  ggTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAGGggcag               <  1:1901352/150‑1 (MQ=255)
                                                                                                                                    tCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAgg                                                                                                                          >  2:293519/1‑41 (MQ=255)
                                                                                                                                    tCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAgg                                                                                                                          <  1:293519/41‑1 (MQ=255)
                                                                                                                                    tCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCAAATCGATGGGATAAGCCATGGCCCCGAGGggcaggc             <  2:988561/150‑1 (MQ=255)
                                                                                                                                             aCGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAGGGGCAGGCACATCAGTa    >  2:1926555/1‑150 (MQ=255)
                                                                                                                                               gccgccGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGGATTCGAGCTTGACGGCGGACAGCTTCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAGGGGCAGGCACATCTGCAtt  >  1:627975/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
ATTCCGCGCTGCAGTTCAAGGCGATCCGCAGTTTGATGATTTGCCGATTGAATTTCGCGGTGACATCGACGCTTTTACCCGCCGGTACGTTCACCCGGCGCACCCGCGGTGCCTCTGGGCCATTACGGAAGGTCACCTTGCACGCCGCCGGGACCTGCCCATAGTTGTTGAGGGTGATGGCGCCAATGTCATAGGCCGTGTCATAGGCGGTGTAGTCGAGCTTGACCCCGGTCAGCTCCTTCTCCACATCGATGGGATAAGCCATGGCCCCGAGGGGCAGGCACATCAGTATT  >  NC_002947/5155229‑5155521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑