Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,169,775	T→A	6.5%	W449R (TGG→AGG)	PP_4547 →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,169,775	0	T	A	6.5%	233.6 / 4.9	74	W449R (TGG→AGG)	PP_4547	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (32/37);  new base A (2/3);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACTGACTGGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAA  >  NC_002947/5169628‑5169911                                                                                                                                                    |                                                                                                                                         actgactgGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTgg                                                                                                                                        <  1:1353924/150‑1 (MQ=255)          cTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCAc                                                                                                                               <  2:434502/150‑1 (MQ=255)            gACCGCACTCGACGCCCTGTAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGTCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCa                                                                                                                             <  1:862408/150‑1 (MQ=255)               cGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCATTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGc                                                                                                                          >  1:1152130/1‑150 (MQ=255)                gCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAGTcc                                                                                                                         <  2:242446/150‑1 (MQ=255)                  aCTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGt                                                                                                                                       <  2:506601/134‑1 (MQ=255)                  aCTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGt                                                                                                                                       >  1:506601/1‑134 (MQ=255)                   cTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGa                                                                                                                      >  1:588046/1‑150 (MQ=255)                   cTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAACGCCTTGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGa                                                                                                                      <  1:1203918/150‑1 (MQ=255)                     tgACGCCCTGCAACTTTCAGCTTGGGACCGCAACGACTTCGGCATGTCCTTGCTCGTCGTCTACCTGAATGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAGTCCTgttc                                                                                                                    <  2:909534/149‑4 (MQ=255)                     cGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGc                                                                                                                    <  2:545963/150‑1 (MQ=255)                      gACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGcc                                                                                                                   >  2:988846/1‑150 (MQ=255)                       aCGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAGTCCTgttcga                                                                                                                  >  1:909534/1‑145 (MQ=38)                       aCGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGccc                                                                                                                  <  1:1093299/150‑1 (MQ=255)                           ccTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTAcc                                                                                                                                    >  1:722728/1‑128 (MQ=255)                           ccTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTAcc                                                                                                                                    <  2:722728/128‑1 (MQ=255)                            cTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTaaa                                                                                                             >  1:1745379/1‑150 (MQ=255)                              gCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATa                                                                                                           <  2:980286/150‑1 (MQ=255)                                  cATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTTGTACCTGCACCAGGCCTGAGCCCCTAAATAACaa                                                                                                       <  1:155323/150‑1 (MQ=255)                                  cATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACaa                                                                                                       <  2:1752250/150‑1 (MQ=255)                                  cATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACaa                                                                                                       <  1:88723/150‑1 (MQ=255)                                    ttCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAgg                                                                                                     >  1:1209496/1‑150 (MQ=255)                                      ccGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGaa                                                                                                   <  2:618182/150‑1 (MQ=255)                                            gggCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCATTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCa                                                                                             <  2:1152130/150‑1 (MQ=255)                                              gCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATg                                                                                           >  2:1359309/1‑150 (MQ=255)                                                   tGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAacac                                                                                                <  1:579664/140‑1 (MQ=255)                                                   tGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAacac                                                                                                >  2:579664/1‑140 (MQ=255)                                                    gAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAATGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACgca                                                                                     >  2:1118110/1‑150 (MQ=255)                                                    gAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACgca                                                                                     <  2:1775907/150‑1 (MQ=255)                                                        cATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACCAGGAACACCCCTGAACGCAGCAt                                                                                 >  1:313197/1‑150 (MQ=255)                                                         aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAgg                                                                                                     <  1:1489063/129‑1 (MQ=255)                                                         aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAgg                                                                                                     >  2:1489063/1‑129 (MQ=255)                                                         aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCAtt                                                                                >  1:1202412/1‑150 (MQ=255)                                                         aTTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACAAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCAtt                                                                                <  1:3706/150‑1 (MQ=255)                                                          ttGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAg                                                                                                      <  2:540653/127‑1 (MQ=255)                                                          ttGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAg                                                                                                      >  1:540653/1‑127 (MQ=255)                                                             ggCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACa                                                                                                        >  1:1720204/1‑122 (MQ=255)                                                             ggCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACa                                                                                                        <  2:1720204/122‑1 (MQ=255)                                                                gAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGaacaac                                                                         <  1:1359309/150‑1 (MQ=255)                                                                      ttCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAgtc                                                                                                                          <  1:774933/95‑3 (MQ=37)                                                                      ttCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGCCTGGTGCAGGTACCAGCGCCAgtc                                                                                                                          >  2:774933/1‑93 (MQ=37)                                                                          ttGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTgg                                                                                                                                        >  2:329179/1‑77 (MQ=255)                                                                          ttGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTgg                                                                                                                                        <  1:329179/77‑1 (MQ=255)                                                                          ttGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGgcgc                                                               >  1:1193562/1‑150 (MQ=255)                                                                           tGGCGTGTACCTGAATGGCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACCCCCATGAACGCAGCATTGAACAACGGCCCGGCGCa                                                              <  2:798941/150‑1 (MQ=255)                                                                                     cTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGaacaa                                                                          >  1:6767/1‑128 (MQ=255)                                                                                     cTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGaacaa                                                                          <  2:6767/128‑1 (MQ=255)                                                                                     cTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCg                                                    >  2:1471133/1‑150 (MQ=255)                                                                                        aaGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTcc                                                 >  2:1011628/1‑150 (MQ=255)                                                                                          tgTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGttgtt                                               <  1:1081293/149‑5 (MQ=255)                                                                                           gTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCGGCGCGCGCCGTCctac                                              <  1:988846/150‑1 (MQ=255)                                                                                            tCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGGGCGCCGttttgca                                             >  2:1081293/1‑144 (MQ=255)                                                                                              aaGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCtactac                                           <  1:1190947/150‑1 (MQ=255)                                                                                                 cgcgcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAgc                                        >  1:1072559/1‑150 (MQ=255)                                                                                                    gcCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGcc                                     >  2:1748270/1‑150 (MQ=255)                                                                                                            ccGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCaa                             >  2:1829174/1‑150 (MQ=255)                                                                                                            ccGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCaa                             <  2:1745379/150‑1 (MQ=255)                                                                                                                        ggCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGc                 <  1:1118110/150‑1 (MQ=255)                                                                                                                        ggCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGc                 <  1:1748270/150‑1 (MQ=255)                                                                                                                           cGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTa              >  1:495328/1‑150 (MQ=255)                                                                                                                            gAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGaa                                                                                                   <  2:1014393/64‑1 (MQ=255)                                                                                                                            gAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGaa                                                                                                   >  1:1014393/1‑64 (MQ=255)                                                                                                                            gAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTAc             <  2:1072559/150‑1 (MQ=255)                                                                                                                              aGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCGTCAACGACCACACTGCGTAccc           <  2:1209496/150‑1 (MQ=255)                                                                                                                               gTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAaca                                                                                                 >  2:235780/1‑63 (MQ=255)                                                                                                                               gTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAaca                                                                                                 <  1:235780/63‑1 (MQ=255)                                                                                                                                  aGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCaa       >  1:966584/1‑150 (MQ=255)                                                                                                                                  aGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCaa       <  2:1202412/150‑1 (MQ=255)                                                                                                                                   gCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAAc      >  2:1194050/1‑150 (MQ=255)                                                                                                                                       aCAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACACTCAACGACCACACTGCGTAACCGCAACTCaa  >  1:271191/1‑150 (MQ=255)                                                                                                                                         aGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGccc                                                                    <  2:796490/82‑1 (MQ=255)                                                                                                                                         aGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGccc                                                                    >  1:796490/1‑82 (MQ=255)                                                                                                                                           gACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCAcc                                  >  1:613773/1‑114 (MQ=255)                                                                                                                                           gACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCAcc                                  <  2:613773/114‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                         ACTGACTGGCTGACCGCACTCGACGCCCTGCAACATTCCGATTGGGCCCGTGAAGCATTGGGCGAAGACTTCCTTGGCGTGTACCTGAAGGTCAAGCGCGCCGAGTACCGCCAGTTCATGGCCGAAGTCAGCGAACAGGACTGGCGCTGGTACCTGCACCAGGCCTGAGCCCCTAAATAACAAGGAACACCCATGAACGCAGCATTGAACAACGGCCCGGCGCAGCGCGCGCCGTCCTACTACAGCGCCACCCTCAACGACCACACTGCGTACCCGCAACTCAA  >  NC_002947/5169628‑5169911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑