BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,277,709	C→A	5.3%	L208M (CTG→ATG)	PP_4652 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,277,709	0	C	A	5.3%	332.5 / 4.5	104	L208M (CTG→ATG)	PP_4652	membrane protein
Reads supporting (aligned to +/- strand): ref base C (49/48); new base A (3/3); total (52/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 106 total aligned reads displayed.

GGTGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCTT  >  NC_002947/5277567‑5277855

ggTGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggc                                                                                                                                             <  1:1309666/150‑1 (MQ=255)

ggTGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCAGCGATTCGATgctggc                                                                                                                                             <  1:1223925/150‑1 (MQ=255)

    ggCGGATTCCTTTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTTATCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCtggctggc                                                                                                                                         <  1:1764978/150‑1 (MQ=255)

    ggCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCtggctggc                                                                                                                                         <  1:1539923/150‑1 (MQ=255)

             cTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTc                                                                                                                                >  1:548220/1‑150 (MQ=255)

              tGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCa                                                                                                                               <  2:548220/150‑1 (MQ=255)

                 aTGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggct                                                                                                                                            >  2:188056/1‑134 (MQ=255)

                 aTGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggct                                                                                                                                            <  1:188056/134‑1 (MQ=255)

                   gCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGc                                                                                                                          >  1:1444948/1‑150 (MQ=255)

                    ccTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCa                                                                                                                         <  1:1309144/150‑1 (MQ=255)

                    ccTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCa                                                                                                                         >  1:1449429/1‑150 (MQ=255)

                     cTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGAATTGTACGCCATTTCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAg                                                                                                                        >  1:1306823/1‑150 (MQ=255)

                      tttGTCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGc                                                                                                                       <  2:527650/150‑1 (MQ=255)

                          gcagcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGc                                                                                                                   <  1:1370992/150‑1 (MQ=255)

                          gcagcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGc                                                                                                                   >  1:192062/1‑150 (MQ=255)

                             gcaTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATGGCTGCTGTCTGGCATTCGTTTCATTCGCAGCCGGCCCg                                                                                                                <  2:222291/150‑1 (MQ=255)

                              caTGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGa                                                                                                               <  2:1098371/150‑1 (MQ=255)

                                tgtgGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGGGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCCCTGCTGGCTGGCATCCGCTTCATGCGCAGCCGGCCCGACa                                                                                                             <  2:1289562/150‑1 (MQ=255)

                                     gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGccc                                                                                                                 <  1:206077/141‑1 (MQ=255)

                                     gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGccc                                                                                                                 >  2:206077/1‑141 (MQ=255)

                                     gtgtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTc                                                                                                        <  2:1553434/150‑1 (MQ=255)

                                       gtACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTgctggc                                                                                                                                             >  1:969224/1‑111 (MQ=255)

                                        tACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGcc                                                                                                                      <  1:1590414/133‑1 (MQ=255)

                                          cGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgcgc                                                                                                   >  1:204843/1‑150 (MQ=255)

                                            gCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCa                                                                                                 >  2:1255185/1‑150 (MQ=255)

                                            gCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGAAACTAACTTGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCa                                                                                                 >  1:1180719/1‑150 (MQ=255)

                                             cccGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCAt                                                                                                >  1:464625/1‑150 (MQ=255)

                                                   ggTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCt                                                                                          >  2:1720301/1‑150 (MQ=255)

                                                      ggCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGa                                                                                       >  1:853089/1‑150 (MQ=255)

                                                       gCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                      <  2:1054650/150‑1 (MQ=255)

                                                       gCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                      <  2:1269512/150‑1 (MQ=255)

                                                       gCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                      >  1:307693/1‑150 (MQ=255)

                                                         cTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCt                                                                                    >  1:1460274/1‑150 (MQ=255)

                                                          ttGTACGCCATTGCCTGCGTGCTGCCCCGGCGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCGTCGAAGCGCTGCTGGCTGGCATCCGCTTCCTTCGCAGCCGGCCCGACATCCTCGGCGCCATCGCGCTGGACCTg                                                                                   <  2:180707/150‑1 (MQ=255)

                                                          ttGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTg                                                                                   >  2:1888534/1‑150 (MQ=255)

                                                          ttGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTg                                                                                   >  2:939393/1‑150 (MQ=255)

                                                          ttGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTg                                                                                   <  1:1720301/150‑1 (MQ=255)

                                                             tACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAt                                                                                                                              <  1:369422/104‑1 (MQ=255)

                                                             tACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAt                                                                                                                              >  2:369422/1‑104 (MQ=255)

                                                             tACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                      <  1:306277/144‑1 (MQ=255)

                                                             tACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGAc                                                                                      >  2:306277/1‑144 (MQ=255)

                                                             tACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTc                                                                                >  2:1497964/1‑150 (MQ=255)

                                                              aCGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATcc                                                                                                          <  2:882829/123‑1 (MQ=255)

                                                              aCGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCg                                                                               >  1:1565656/1‑150 (MQ=255)

                                                              aCGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCg                                                                               >  2:576698/1‑150 (MQ=255)

                                                              aCGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCCCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACAGcc                                                                                                          >  1:882829/1‑123 (MQ=255)

                                                                gCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGcc                                                                             >  1:665030/1‑150 (MQ=255)

                                                                gCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGcc                                                                             <  2:853089/150‑1 (MQ=255)

                                                                 ccATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCgct                                                                                                                                                   >  2:315081/1‑79 (MQ=255)

                                                                  cATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAt                                                                                                                              <  1:1884920/99‑1 (MQ=255)

                                                                  cATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCAt                                                                                                                              >  2:1884920/1‑99 (MQ=255)

                                                                      gCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTAttg                                                                       >  2:1759698/1‑150 (MQ=255)

                                                                        cTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACCTCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTAttgtt                                                                     <  1:535498/150‑1 (MQ=255)

                                                                             tacTGCCCCTGAGCCCGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCg                                                                <  2:1504213/148‑1 (MQ=255)

                                                                                tGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCt                                                                                                                                  <  1:392904/81‑1 (MQ=255)

                                                                                tGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgcgc                                                                                                   <  2:506096/112‑1 (MQ=255)

                                                                                tGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGgcgc                                                                                                   >  1:506096/1‑112 (MQ=255)

                                                                                  aCCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGcc                                                           >  2:411955/1‑150 (MQ=255)

                                                                                    cctgccccTGCAGGCGCGCTGCCAGGTCTCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCAc                                                         <  2:1565656/144‑1 (MQ=255)

                                                                                    ccTGAGCCTGCAGGCGCGCGGCCAGGCGAGGCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCAc                                                         >  1:1020494/1‑150 (MQ=255)

                                                                                       gAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCgt                                                      <  2:965700/150‑2 (MQ=255)

                                                                                           cTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCTAATCGCTCCTGTCTGGCATCCGCTTCATTCGCAGCCGGCCCGCCATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTCTCAGGCGCACCCACAGCCAtg                                                  >  2:415560/1‑150 (MQ=255)

                                                                                             gCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtgct                                                >  2:823540/1‑150 (MQ=255)

                                                                                               aGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTtgctgc                                              >  2:1417742/1‑150 (MQ=255)

                                                                                                ggCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGcc                                             >  1:1012743/1‑150 (MQ=255)

                                                                                                  cgcgcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCgg                                           <  1:1255185/150‑1 (MQ=255)

                                                                                                     gcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTc                                                                                >  2:1721819/1‑110 (MQ=255)

                                                                                                     gcgGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTc                                                                                <  1:1721819/110‑1 (MQ=255)

                                                                                                         ccAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGc                                    <  2:1095971/150‑1 (MQ=255)

                                                                                                         ccAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGc                                    <  2:665030/150‑1 (MQ=255)

                                                                                                          cAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGc                                                      >  1:951541/1‑131 (MQ=255)

                                                                                                          cAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGc                                                      <  2:951541/131‑1 (MQ=255)

                                                                                                          cAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGcc                                   >  1:552064/1‑150 (MQ=255)

                                                                                                           aGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCa                                  >  1:805872/1‑150 (MQ=255)

                                                                                                            ggTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCaa                                 <  2:1926519/150‑1 (MQ=255)

                                                                                                             ttCGCCCAGCGCGGGGGCCCCAGCATGGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGCCATCCTCGGCGCCATCTCGCTGGTCCTGTTCGCCGTCTTGTTCGGCGGAGCCACCGCCTTGCTGCCGGTCTTTGCCAAg                                >  2:1248569/2‑150 (MQ=255)

                                                                                                              tCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAgg                               >  1:1147446/1‑150 (MQ=255)

                                                                                                                 cccAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACa                            >  1:624216/1‑150 (MQ=255)

                                                                                                                  ccAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGGCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACAt                           >  1:1024210/1‑150 (MQ=255)

                                                                                                                  ccAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACAt                           >  1:183958/1‑150 (MQ=255)

                                                                                                                   cAGCGCGGGCGCGCCAGCATCCAATCCCTGCCGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGCCCTGTTCGCCGTATTGTTCGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAAGACATc                          <  2:1444948/150‑1 (MQ=255)

                                                                                                                    aGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATcc                         >  2:1753418/1‑150 (MQ=255)

                                                                                                                    aGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATcc                         <  2:183958/150‑1 (MQ=255)

                                                                                                                    aGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCAACCGCCTTGCTGCCGGTATTTGCCAAGGACAGcc                         <  2:1449429/150‑1 (MQ=255)

                                                                                                                      cgcgGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCctg                       <  1:1417742/150‑1 (MQ=255)

                                                                                                                        cgGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCctgct                     <  1:939393/150‑1 (MQ=255)

                                                                                                                          ggCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGa                   <  2:794238/150‑1 (MQ=255)

                                                                                                                            ccagcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAgg                               <  2:230941/133‑1 (MQ=255)

                                                                                                                            ccagcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAgg                               >  1:230941/4‑136 (MQ=255)

                                                                                                                             cagcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGa                                                              >  2:572480/3‑104 (MQ=255)

                                                                                                                             cagcCAGCAGCGATTCGATGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGa                                                              <  1:572480/102‑1 (MQ=255)

                                                                                                                                ccAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGc             <  2:805872/150‑1 (MQ=255)

                                                                                                                                 cAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCggggc            >  1:1357261/1‑148 (MQ=255)

                                                                                                                                     aTCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGGCGAta        <  2:1357261/150‑2 (MQ=255)

                                                                                                                                     aTCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATg        <  1:1553218/150‑1 (MQ=255)

                                                                                                                                         aaTCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGa                   <  2:1522382/135‑1 (MQ=255)

                                                                                                                                         aaTCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGa                   >  1:1522382/1‑135 (MQ=255)

                                                                                                                                          aTCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCt   <  2:267279/150‑1 (MQ=255)

                                                                                                                                           tCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCtt  >  1:1188498/1‑150 (MQ=255)

                                                                                                                                           tCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCtt  <  2:1492178/150‑1 (MQ=255)

GGTGGGCGGATTCCTGTATGCCTTTGGCAGCATGTGGGTGTACGGCCCGACGGTGGCCTTGTACGCCATTGCCTGCGTGCTGACCCTGAGCCTGCAGGCGCGCGGCCAGGTCGCCCAGCGCGGGCGCGCCAGCATCGAATCGCTGCTGGCTGGCATCCGCTTCATTCGCAGCCGGCCCGACATCCTCGGCGCCATCTCGCTGGACCTGTTCGCCGTATTGTTGGGCGGAGCCACCGCCTTGCTGCCGGTATTTGCCAAGGACATCCTGCTGACCGGGCCGATGGGCCTT  >  NC_002947/5277567‑5277855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`