Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,307,459 T→C 11.3% intergenic (‑160/+26) recC ← / ← PP_5SG multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit/5S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,307,4590TC11.3% 133.3 / 8.2 53intergenic (‑160/+26)recC/PP_5SGmultifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (2/4);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

GCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGAT  >  NC_002947/5307318‑5307571
                                                                                                                                             |                                                                                                                
gcTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGAt                                                                                                          <  1:865945/150‑1 (MQ=255)
   gATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTATCTGCGCACGCAGGTAgg                                                                                                       <  1:1290842/150‑1 (MQ=255)
    aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAggg                                                                                                      <  2:1332201/150‑1 (MQ=255)
    aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAggg                                                                                                      <  2:1356172/150‑1 (MQ=255)
         tGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGtttt                                                                                                 >  1:1427005/1‑150 (MQ=255)
         tGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGtttt                                                                                                 >  2:1630859/1‑150 (MQ=255)
               gggAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGaaa                                                                                           <  2:580148/150‑1 (MQ=255)
               gggAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGaaa                                                                                           >  1:982030/1‑150 (MQ=255)
               gggAACGATATCACGCGTACATGAAACGGCCGAATGCCATAAGGCTGGGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGaaa                                                                                           >  1:932965/1‑150 (MQ=255)
                 gAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTgtctttgt                                                                                         <  1:1863806/150‑8 (MQ=38)
                 gAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATg                                                                                         >  2:1064811/1‑150 (MQ=255)
                  aaCGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGa                                                                                        >  2:1830769/1‑150 (MQ=255)
                    cGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAAt                                                                                      <  1:1830769/150‑1 (MQ=255)
                      atatCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCt                                                                                    >  1:1632241/1‑150 (MQ=255)
                         tCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGa                                                                                 >  2:1808222/1‑150 (MQ=255)
                         tCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGa                                                                                 <  1:1459871/150‑1 (MQ=255)
                          cACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGAc                                                                                <  1:1064811/150‑1 (MQ=255)
                          cACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGAc                                                                                <  2:982030/150‑1 (MQ=255)
                               gTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTATCTGCGCACGCAGGTAggg                                                                                                      >  1:1169333/1‑123 (MQ=38)
                               gTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTATCTGCGCACGCAGGTAggg                                                                                                      <  2:1169333/123‑1 (MQ=38)
                                    tGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTgt                                                                                               <  2:721473/125‑2 (MQ=38)
                                    tGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTgt                                                                                               >  1:721473/1‑124 (MQ=38)
                                    tGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCa                                                                                                             <  2:1625485/111‑1 (MQ=255)
                                    tGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCa                                                                                                             >  1:1625485/1‑111 (MQ=255)
                                     gAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAAt                                                                                      <  1:102847/133‑1 (MQ=255)
                                     gAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAAt                                                                                      >  2:102847/1‑133 (MQ=255)
                                     gAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTAct                                                                     <  1:213439/150‑1 (MQ=255)
                                           gCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGTAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTcaca                                                               <  2:1685463/150‑1 (MQ=255)
                                                   ccATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGaaa                                                       <  1:1026714/150‑1 (MQ=255)
                                                        aGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCca                                                  <  1:1283094/150‑1 (MQ=255)
                                                                 gAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCAt                                         >  1:2774/1‑150 (MQ=255)
                                                                    cGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCgg                                      <  1:334810/150‑1 (MQ=255)
                                                                    cGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCgg                                      >  2:665908/1‑150 (MQ=255)
                                                                      gCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTTACATGGGGAAACCCCACACTACCATCGGCg                                    <  1:1808222/150‑1 (MQ=255)
                                                                           gCCCGCTCCCGCAGCGATTCGCGCAGGTCGGGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATgg                                                            <  1:451873/121‑1 (MQ=255)
                                                                           gCCCGCTCCCGCAGCGATTCGCGCAGGTCGGGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATgg                                                            >  2:451873/1‑121 (MQ=255)
                                                                             ccGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGAt                                  <  1:1757491/145‑1 (MQ=255)
                                                                             ccGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGAt                                  >  2:1757491/1‑145 (MQ=255)
                                                                              cGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATc                                                                                     <  1:1372788/93‑1 (MQ=255)
                                                                              cGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATc                                                                                     >  2:1372788/1‑93 (MQ=255)
                                                                                cTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGtt                          <  2:932965/150‑1 (MQ=255)
                                                                                     gCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTGact                     >  2:1704118/1‑150 (MQ=255)
                                                                                        gCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCactact                  <  1:665908/150‑1 (MQ=40)
                                                                                           aTTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCGCATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAg               >  2:1422511/1‑150 (MQ=255)
                                                                                               gcgcAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCg           >  1:1836101/1‑150 (MQ=38)
                                                                                                cgcAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgg          <  2:1632241/150‑1 (MQ=37)
                                                                                                cgcAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgg          >  1:1348317/1‑150 (MQ=37)
                                                                                                     gTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgggatgg     >  2:149162/1‑150 (MQ=34)
                                                                                                     gTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgggatgg     >  1:1889519/1‑150 (MQ=34)
                                                                                                       cGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgggatggga   >  1:1114435/1‑150 (MQ=32)
                                                                                                        gAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCCCATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgggatgtgat  <  1:1422511/150‑1 (MQ=17)
                                                                                                         aGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTAc                                                                      <  1:1302546/81‑1 (MQ=32)
                                                                                                         aGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTAc                                                                      >  2:1302546/1‑81 (MQ=32)
                                                                                                                                             |                                                                                                                
GCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGCGGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGAT  >  NC_002947/5307318‑5307571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑