Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,313,324 C→G 9.2% intergenic (‑595/+119) PP_16SG ← / ← msrQ 16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,313,3240CG 9.2% 163.0 / 8.8 55intergenic (‑595/+119)PP_16SG/msrQ16S ribosomal RNA/methionine sulfoxide reductase heme‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (23/26);  new base G (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

TGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCCGCGC  >  NC_002947/5313183‑5313472
                                                                                                                                             |                                                                                                                                                    
tGGAAGTGGGGTGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCgcggc                                                                                                                                              <  2:1011682/150‑1 (MQ=255)
tGGAAGTGGGGCGCATTATAAGGGGATTCGTGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCgcggc                                                                                                                                              <  1:1009537/150‑1 (MQ=255)
tGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCAAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCgcggc                                                                                                                                              >  2:1057765/1‑150 (MQ=255)
   aaGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCt                                                                                                                                           <  2:806987/150‑1 (MQ=255)
    aGTGGCGCGCATTGTAAGGGGCTTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                                          <  2:1567058/150‑1 (MQ=14)
           cgcATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTca                                                                                                                                   >  1:613980/1‑150 (MQ=255)
                 gtaAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACg                                                                                                                             >  2:1329116/2‑150 (MQ=255)
                      gggATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCAAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCaga                                                                                                                        <  1:1057765/150‑1 (MQ=255)
                        gagtCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAgaga                                                                                                                      >  2:487004/4‑150 (MQ=255)
                                   gCGTCAACCTTTAATTGCAAGAAAGTGAAATATAAGGGGAAATCCCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCGATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                           <  1:959771/150‑1 (MQ=255)
                                    cGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCggg                                                                                                          <  2:387803/150‑1 (MQ=255)
                                            tttAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                          <  2:926452/110‑1 (MQ=255)
                                            tttAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                          >  1:926452/1‑110 (MQ=255)
                                                     aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                                      <  1:351581/105‑1 (MQ=255)
                                                     aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                                      >  2:351581/1‑105 (MQ=255)
                                                     aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                           >  2:796220/1‑132 (MQ=255)
                                                     aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCgg                                                                                                           <  1:796220/132‑1 (MQ=255)
                                                     aaGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCCCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCAtt                                                                                         >  2:1483534/1‑150 (MQ=255)
                                                      aGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTg                                                                                        <  1:1754622/150‑1 (MQ=255)
                                                       gAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGc                                                                                       >  2:1464832/1‑150 (MQ=255)
                                                            tGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAtcc                                                                                  >  2:1694931/1‑149 (MQ=255)
                                                               aaTATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGTTTCCTGTGTATGACGGCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCaa                                                                               <  2:1441979/150‑1 (MQ=21)
                                                                 tataAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCGCAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt                                                                                    <  1:867233/143‑1 (MQ=255)
                                                                 tataAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCGCAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt                                                                                    >  2:867233/1‑143 (MQ=255)
                                                                 tataAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGa                                                                             >  2:168470/1‑150 (MQ=255)
                                                                               ccGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAg                                                               >  2:451445/1‑150 (MQ=255)
                                                                                   aTCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGcccc                                                           >  1:1442639/1‑150 (MQ=255)
                                                                                         gCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGccccacccca                                                     <  1:487004/150‑1 (MQ=255)
                                                                                           tGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCATAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCAct                                                   <  1:1130678/150‑1 (MQ=255)
                                                                                             ccAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCAGCACACGTCCATTGCTATCTCAAGACAGGCCCTTGGCAGCcccaccccacccc                                                 >  1:611093/1‑147 (MQ=37)
                                                                                                  tgtTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCCCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAAc                                            <  1:1483534/150‑1 (MQ=255)
                                                                                                            aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTAccc                                  >  2:1068304/1‑150 (MQ=255)
                                                                                                            aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTAccc                                  >  1:1739001/1‑150 (MQ=255)
                                                                                                            aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTAccc                                  >  1:816233/1‑150 (MQ=255)
                                                                                                            aacaGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTAccc                                  <  2:816233/150‑1 (MQ=255)
                                                                                                                 tttCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAgg                                                                         <  1:347600/106‑1 (MQ=255)
                                                                                                                 tttCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAgg                                                                         >  2:347600/1‑106 (MQ=255)
                                                                                                                  ttCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTAc                            <  2:613980/150‑1 (MQ=255)
                                                                                                                  ttCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTAc                            <  1:1329116/150‑1 (MQ=255)
                                                                                                                      gcgcCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTgg                        >  2:1072676/1‑150 (MQ=255)
                                                                                                                      gcgcCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTgg                        >  2:1072639/1‑150 (MQ=255)
                                                                                                                        gcCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCa                      >  1:972094/1‑150 (MQ=255)
                                                                                                                             tGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCtt                 <  1:1775887/150‑1 (MQ=255)
                                                                                                                             tGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCtt                 >  2:1775887/1‑150 (MQ=255)
                                                                                                                              ggggtCGAGCGCCGCACGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg                <  2:409770/146‑1 (MQ=255)
                                                                                                                              gagaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCCCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTg                <  1:1323012/150‑1 (MQ=255)
                                                                                                                               ggaTCGACCGCCGCCCGCGCGGCGCTCCCTCGCAGAGACGACAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCCCCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgt               <  2:611093/149‑2 (MQ=255)
                                                                                                                               agaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTgg               <  1:451445/150‑1 (MQ=255)
                                                                                                                                gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTggg              <  1:1072639/150‑1 (MQ=255)
                                                                                                                                gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTggg              <  1:1072676/150‑1 (MQ=255)
                                                                                                                                 aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTg                         <  1:560381/138‑1 (MQ=255)
                                                                                                                                 aTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTg                         >  2:560381/1‑138 (MQ=255)
                                                                                                                                  tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt                                                                                    >  2:1548657/1‑78 (MQ=25)
                                                                                                                                  tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTAt                                                                                    <  1:1548657/78‑1 (MQ=25)
                                                                                                                                      gcgcCGCCCGCGCGGCGCTCTATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAgcgc        <  1:1068304/150‑1 (MQ=255)
                                                                                                                                            cccgcGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCCgcgc  >  1:1290188/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TGGAAGTGGGGCGCATTATAAGGGGATTCGAGAGGGCGTCAACCTTTAATTTCAAGAAAGTGAAATATAAGGGGAAATACCGTATCGGGGCTGCCAAGTGTTCGCCACAACAGTTTCAGCGCCTATGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGACGCCAGAGAAACAAAGGCGGGCCCTCTCCACAGCCATTGCTATCTCAAGACAGGCCCTTGGCAGCCCCACCCCACTCTCAAACCGCCCTACCCTGCTACCTGGCAACCTTGGGAAGCGCCCGCGC  >  NC_002947/5313183‑5313472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑