Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 354,876 A→C 7.4% F100C (TTC→TGC)  cbcW ← choline/betaine/carnitine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947354,8760AC 7.4% 187.5 / 6.4 67F100C (TTC→TGC) cbcWcholine/betaine/carnitine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (30/32);  new base C (2/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

AACAGGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAA  >  NC_002947/354729‑355017
                                                                                                                                                   |                                                                                                                                             
aaCAGCGTCAGGGCAGGCACCAGGGAGACGCAGGTGCGCACCGTCTGCATCATGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAg                                                                                                                                             <  2:871181/150‑1 (MQ=255)
    gggtcagggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGa                                                                                                                                         <  1:916929/150‑1 (MQ=255)
       tcagggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCt                                                                                                                                      >  1:808649/1‑150 (MQ=255)
            gtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGcc                                                                                                                                 >  1:652713/1‑150 (MQ=255)
             tAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCa                                                                                                                                <  2:731990/150‑1 (MQ=255)
               gggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAgg                                                                                                                              <  1:1191230/150‑1 (MQ=255)
                ggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGCAGGTCACCTTCGCCAcgg                                                                                                                             <  1:1273335/150‑1 (MQ=255)
                 gATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                            >  2:1221251/1‑150 (MQ=255)
                 gATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                            >  2:1630911/1‑150 (MQ=255)
                       gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCAt                                                                                                                      >  2:1059188/1‑150 (MQ=255)
                        tggACGAATGTGGGCATCGTCTGCATCATGTCGAGCGCCGGGCGCATGGCTGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                                     <  2:598821/148‑1 (MQ=255)
                             gAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                <  1:1394760/150‑1 (MQ=255)
                             gAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                >  1:852443/1‑150 (MQ=255)
                                 gTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                            <  2:343695/150‑1 (MQ=255)
                                    ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAg                                                                                                         >  1:252018/1‑150 (MQ=255)
                                     gCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGt                                                                                                        >  1:1916530/1‑150 (MQ=255)
                                      cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                >  2:1829940/1‑141 (MQ=255)
                                      cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                <  1:1829940/141‑1 (MQ=255)
                                             tGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAgg                                                                                                >  1:140149/1‑150 (MQ=255)
                                             tGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAgg                                                                                                <  2:1916530/150‑1 (MQ=255)
                                                 tCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGa                                                                                            <  1:1059188/150‑1 (MQ=255)
                                                 tCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGa                                                                                            <  1:595876/150‑1 (MQ=255)
                                                   aGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGGCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAgg                                                                                          <  2:190991/150‑1 (MQ=255)
                                                    ggTCGAGCACCGGGCGCATGGCGGTCTAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCAAGTAACCCAGGTggagga                                                                                         >  1:646777/1‑150 (MQ=255)
                                                        gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACACAGGTTGAGGATcag                                                                                     >  1:79853/1‑150 (MQ=255)
                                                        gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                            <  2:111227/111‑1 (MQ=38)
                                                        gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                            <  2:111196/111‑1 (MQ=38)
                                                        gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                            >  1:111196/1‑111 (MQ=38)
                                                        gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                            >  1:111227/1‑111 (MQ=38)
                                                           cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                  <  1:348774/150‑1 (MQ=255)
                                                           cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                  >  2:348774/1‑150 (MQ=255)
                                                           cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                  >  2:1336916/1‑150 (MQ=255)
                                                                     aTGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCTTCCCATTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                        >  1:101525/1‑150 (MQ=255)
                                                                          ggTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                   <  1:1630911/150‑1 (MQ=255)
                                                                           gTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                   <  2:1355364/133‑1 (MQ=255)
                                                                           gTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                   >  1:1355364/1‑133 (MQ=255)
                                                                             gtaGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                >  2:1335014/2‑150 (MQ=255)
                                                                              taGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAAt                                                               <  1:1336916/150‑1 (MQ=255)
                                                                                gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                <  2:390352/147‑1 (MQ=255)
                                                                                gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                >  1:390352/1‑147 (MQ=255)
                                                                                gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGGTGGGTATCATTAGAAACGACAGGGCGACAAATGc                                                             >  2:1070172/1‑150 (MQ=255)
                                                                                               cgcgGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACtt                                              <  1:1335014/150‑1 (MQ=255)
                                                                                                gcggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                   <  2:1130923/128‑1 (MQ=255)
                                                                                                gcggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                   >  1:1130923/1‑128 (MQ=255)
                                                                                                    cgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCt                                         <  1:219436/150‑1 (MQ=255)
                                                                                                     gAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATCGTTTACTGCAAGTAACCCAGGTCGCGGATCACAAGAAACGACACGGCGACAAATGCGGTCAGTGGCCACTTGGGCTg                                        >  2:118016/1‑150 (MQ=255)
                                                                                                        gATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                                     >  2:1844144/1‑150 (MQ=255)
                                                                                                          tGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                        >  2:951044/1‑113 (MQ=255)
                                                                                                          tGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                        <  1:951044/113‑1 (MQ=255)
                                                                                                            cccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGaaa                                 <  2:140149/150‑1 (MQ=255)
                                                                                                             ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAAt                                >  2:393521/1‑150 (MQ=255)
                                                                                                             ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAAt                                >  1:622306/1‑150 (MQ=255)
                                                                                                               aGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATgc                              <  1:1844144/150‑1 (MQ=255)
                                                                                                                   gCACGCCGATCACCACACATACCACCGTGGCGACGGTCGTCCGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCa                          <  1:1070172/150‑1 (MQ=255)
                                                                                                                   gCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgcgc                                          >  1:284873/1‑134 (MQ=255)
                                                                                                                   gCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgcgc                                          <  2:284873/134‑1 (MQ=255)
                                                                                                                    cACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAg                         <  2:808649/150‑1 (MQ=255)
                                                                                                                          gATCACCACACATACAACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGATAGGGCGACAAATGCGGTCAGTGCCCACTTGCCCTGGATGAATTGCGCCAGggcggc                   <  2:79853/150‑1 (MQ=255)
                                                                                                                                      tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                              >  2:1843629/1‑79 (MQ=255)
                                                                                                                                      tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                              <  1:1843629/79‑1 (MQ=255)
                                                                                                                                         caccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGGATGGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAg    >  1:18777/1‑150 (MQ=255)
                                                                                                                                           ccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaa  <  2:652713/150‑1 (MQ=255)
                                                                                                                                           ccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaa  <  1:1221251/150‑1 (MQ=255)
                                                                                                                                              tGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGcc                                                   >  2:328493/1‑98 (MQ=255)
                                                                                                                                              tGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGcc                                                   <  1:328493/98‑1 (MQ=255)
                                                                                                                                                  gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                              <  1:1908593/83‑1 (MQ=255)
                                                                                                                                                  gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                              >  2:1908593/1‑83 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AACAGGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAA  >  NC_002947/354729‑355017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑