Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,643,313 C→T 5.3% intergenic (+47/‑76) dbpA → / → yhiN 23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,643,3130CT 5.3% 335.3 / 4.7 111intergenic (+47/‑76)dbpA/yhiN23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (51/54);  new base T (3/3);  total (54/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 111 total aligned reads displayed.

CAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTA  >  NC_002947/5643165‑5643461
                                                                                                                                                    |                                                                                                                                                    
cAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCa                                                                                                                                                     >  2:1080772/1‑150 (MQ=255)
cAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCa                                                                                                                                                     >  1:148258/1‑150 (MQ=255)
 aGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAg                                                                                                                                                    >  1:385404/1‑150 (MQ=255)
  ggCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGt                                                                                                                                                   <  2:935696/150‑1 (MQ=255)
  ggCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGt                                                                                                                                                   <  2:148258/150‑1 (MQ=255)
     gTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGaa                                                                                                                                                <  2:850659/150‑1 (MQ=255)
       ttGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAATCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATg                                                                                                                                              <  2:1145728/150‑1 (MQ=255)
           ggCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCGGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCAcc                                                                                                                                          <  2:439741/150‑1 (MQ=255)
              cGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATc                                                                                                                                       >  2:1643754/1‑150 (MQ=255)
                 ggAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGCTCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCa                                                                                                                                    <  1:1877013/150‑1 (MQ=255)
                 ggAGCGGGAGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTACGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCa                                                                                                                                    <  2:277480/150‑1 (MQ=255)
                  gAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGAGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCAt                                                                                                                                   >  2:1651321/1‑150 (MQ=255)
                    gCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTc                                                                                                                                 <  2:1455606/150‑1 (MQ=255)
                        gcgcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                             <  1:324699/150‑1 (MQ=255)
                          gcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGt                                                                                                                           <  1:1080772/150‑1 (MQ=255)
                             ggCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGaa                                                                                                                        <  1:1263348/150‑1 (MQ=255)
                             ggCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTaaa                                                                                                                        <  2:466501/150‑1 (MQ=25)
                               ccAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCgg                                                                                                                             >  1:514291/1‑143 (MQ=25)
                                cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAccc                                                                                                                     >  1:868399/1‑150 (MQ=255)
                                 aaGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCg                                                                                                                    >  1:16768/1‑150 (MQ=255)
                                  aGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                                                   >  2:1848944/1‑150 (MQ=255)
                                   gCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCGGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCt                                                                                                                  >  2:1835771/1‑150 (MQ=255)
                                       gCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCca                                                                                                              >  1:878504/1‑150 (MQ=255)
                                         cATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGaa                                                                                                                                                >  1:1382517/1‑114 (MQ=255)
                                         cATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGaa                                                                                                                                                <  2:1382517/114‑1 (MQ=255)
                                          aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGt                                                                                                                                                   >  1:1590430/1‑110 (MQ=255)
                                          aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGt                                                                                                                                                   <  2:1590430/110‑1 (MQ=255)
                                          aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAg                                                                                                           >  1:334548/1‑150 (MQ=255)
                                            gCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                         <  2:16768/150‑1 (MQ=255)
                                              aGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAATCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                       <  1:396443/150‑3 (MQ=17)
                                               gCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                      <  2:878504/150‑1 (MQ=255)
                                                 ggCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAg                                                                                                    <  1:1581580/150‑1 (MQ=255)
                                                   cTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCa                                                                                                  >  1:853947/1‑150 (MQ=255)
                                                     gAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCAGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                                                <  1:613667/150‑1 (MQ=255)
                                                      aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGTGTCAGCAttt                                                                                               <  1:469904/150‑1 (MQ=255)
                                                      aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                               <  2:334548/150‑1 (MQ=255)
                                                      aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                               <  1:1848944/150‑1 (MQ=255)
                                                       aCAGCGGCAAGATCAAGGGTCGTGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggg                                                                                                         <  2:1746838/139‑1 (MQ=14)
                                                       aCAGCGGCAAGATCAAGGGTCGTGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggg                                                                                                         >  1:1746838/1‑139 (MQ=14)
                                                       aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                               <  1:1872606/149‑1 (MQ=255)
                                                       aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                               >  2:1872606/1‑149 (MQ=255)
                                                       aCAGCGGCAAGATCAAGGGTCGAGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTTAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                              <  1:1651321/150‑1 (MQ=255)
                                                         aGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaa                                                                                            >  1:873670/1‑150 (MQ=255)
                                                           gggCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                          <  2:1755765/149‑1 (MQ=255)
                                                           cGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATTCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                          <  1:321278/150‑1 (MQ=255)
                                                             gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAAc                                                                                                                       >  1:896464/1‑119 (MQ=14)
                                                             gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGt                                                                                                                                                   >  1:1869783/1‑91 (MQ=255)
                                                             gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCt                                                                                        >  1:603392/1‑150 (MQ=255)
                                                               aaGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGa                                                                                      <  1:1066590/150‑1 (MQ=255)
                                                                 gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAATGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAg                                                                                    <  1:502558/150‑1 (MQ=255)
                                                                 gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAACGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAg                                                                                    <  2:944511/150‑1 (MQ=255)
                                                                   tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTACCAAAGGGGTCAGTAAGTCTAATCTGATGGt                                                                                  >  2:902131/1‑150 (MQ=38)
                                                                    cAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                          <  1:1294955/141‑1 (MQ=255)
                                                                    cAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                          >  2:1294955/1‑141 (MQ=255)
                                                                     aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                             >  1:280726/1‑105 (MQ=255)
                                                                     aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                             <  2:280726/105‑1 (MQ=255)
                                                                     aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTc                                                                                >  2:113195/1‑150 (MQ=255)
                                                                             cgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAATGTTCATACCGTg                                                                        <  1:1579176/150‑1 (MQ=255)
                                                                                 cTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACt                                                                    >  2:1102147/1‑150 (MQ=255)
                                                                                        tCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                     <  1:1149568/110‑1 (MQ=255)
                                                                                        tCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                     >  2:1149568/1‑110 (MQ=255)
                                                                                           gCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGt                                                          >  1:1868397/1‑150 (MQ=255)
                                                                                              ttATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGat                                                       >  2:1063889/1‑150 (MQ=255)
                                                                                                  cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATTCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                   <  1:902131/150‑1 (MQ=255)
                                                                                                  cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                   <  1:113195/150‑1 (MQ=255)
                                                                                                  cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                   <  1:1604503/150‑1 (MQ=255)
                                                                                                   tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                  >  1:827929/1‑150 (MQ=255)
                                                                                                   tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                  >  2:1468959/1‑150 (MQ=255)
                                                                                                   tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                  >  1:618875/1‑150 (MQ=255)
                                                                                                    aaaGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCt                                                 >  2:847168/1‑150 (MQ=255)
                                                                                                      aGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                               <  1:1835771/150‑1 (MQ=255)
                                                                                                       gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTgg                                              >  1:555802/1‑150 (MQ=255)
                                                                                                        ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTcgc                                                                                                                               >  1:480479/1‑68 (MQ=255)
                                                                                                        ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTcgc                                                                                                                               <  2:480479/68‑1 (MQ=255)
                                                                                                        ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAATGTTCATACCGTGCACTCCACCGACGTGCTCATCCTTggc                                             >  1:745381/1‑150 (MQ=255)
                                                                                                        ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACAAAATCATCCTTggc                                             >  1:298678/1‑150 (MQ=255)
                                                                                                          aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCGCCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggaga                                           >  1:1868082/1‑147 (MQ=255)
                                                                                                            gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                                            <  2:123515/131‑1 (MQ=255)
                                                                                                            gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                                            >  1:123515/1‑131 (MQ=255)
                                                                                                                  cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGgcgccggcgccg                                   >  1:1848435/1‑150 (MQ=255)
                                                                                                                      cTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCgg                               <  1:319430/150‑1 (MQ=255)
                                                                                                                       tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCAc                                                                <  2:141889/116‑1 (MQ=255)
                                                                                                                       tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCAc                                                                >  1:141889/1‑116 (MQ=255)
                                                                                                                         tAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCt                            <  1:557180/150‑1 (MQ=255)
                                                                                                                            gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                         <  2:78337/150‑1 (MQ=255)
                                                                                                                              gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                       >  2:1454957/1‑150 (MQ=255)
                                                                                                                              gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                       <  1:55852/150‑1 (MQ=255)
                                                                                                                              gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                       <  2:385404/150‑1 (MQ=255)
                                                                                                                                  gTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgcgc                   >  2:756625/1‑150 (MQ=255)
                                                                                                                                  gTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgcgc                   >  2:114333/1‑150 (MQ=255)
                                                                                                                                   tttACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGcc                  <  1:1624292/150‑1 (MQ=255)
                                                                                                                                      aCCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAg               <  2:1184825/150‑1 (MQ=255)
                                                                                                                                        ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                      <  2:854790/61‑1 (MQ=255)
                                                                                                                                        ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                      >  1:854790/1‑61 (MQ=255)
                                                                                                                                        ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt             >  2:522106/1‑150 (MQ=255)
                                                                                                                                        ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                       <  2:1823008/60‑3 (MQ=18)
                                                                                                                                          gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCa           <  2:1848435/150‑1 (MQ=255)
                                                                                                                                          gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTCCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCAGGCCTGATGTGGGCCTAGCTCa           >  2:1079027/1‑150 (MQ=255)
                                                                                                                                                  ggCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGt   >  1:25031/1‑150 (MQ=255)
                                                                                                                                                   gCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTCCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTa  <  1:1079027/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTA  >  NC_002947/5643165‑5643461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑