Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,643,325 C→T 5.9% intergenic (+59/‑64) dbpA → / → yhiN 23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,643,3250CT 5.9% 297.8 / 4.7 100intergenic (+59/‑64)dbpA/yhiN23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (46/48);  new base T (3/3);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 104 total aligned reads displayed.

GGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGT  >  NC_002947/5643176‑5643474
                                                                                                                                                     |                                                                                                                                                     
ggCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCGGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCAcc                                                                                                                                                       <  2:439741/150‑1 (MQ=255)
      ggAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGCTCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCa                                                                                                                                                 <  1:1877013/150‑1 (MQ=255)
      ggAGCGGGAGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTACGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCa                                                                                                                                                 <  2:277480/150‑1 (MQ=255)
       gAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGAGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCAt                                                                                                                                                >  2:1651321/1‑150 (MQ=255)
         gCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTc                                                                                                                                              <  2:1455606/150‑1 (MQ=255)
             gcgcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          <  1:471263/150‑1 (MQ=255)
             gcgcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          <  1:324699/150‑1 (MQ=255)
             gcgcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          <  1:975716/150‑1 (MQ=255)
               gcTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGt                                                                                                                                        <  1:1080772/150‑1 (MQ=255)
                  ggCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGaa                                                                                                                                     <  1:1263348/150‑1 (MQ=255)
                  ggCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTaaa                                                                                                                                     <  2:466501/150‑1 (MQ=25)
                    ccAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCgg                                                                                                                                          >  1:514291/1‑143 (MQ=25)
                     cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAccc                                                                                                                                  >  1:868399/1‑150 (MQ=255)
                      aaGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCg                                                                                                                                 >  1:16768/1‑150 (MQ=255)
                       aGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                                                                >  2:1848944/1‑150 (MQ=255)
                        gCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCGGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCt                                                                                                                               >  2:1835771/1‑150 (MQ=255)
                            gCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCca                                                                                                                           >  1:878504/1‑150 (MQ=255)
                               aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAg                                                                                                                        >  1:334548/1‑150 (MQ=255)
                                 gCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                                      <  2:16768/150‑1 (MQ=255)
                                   aGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAATCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                                    <  1:396443/150‑3 (MQ=17)
                                    gCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                                   <  2:878504/150‑1 (MQ=255)
                                      ggCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAg                                                                                                                 <  1:1581580/150‑1 (MQ=255)
                                        cTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCa                                                                                                               >  1:853947/1‑150 (MQ=255)
                                          gAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCAGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                                                             <  1:613667/150‑1 (MQ=255)
                                           aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGTGTCAGCAttt                                                                                                            <  1:469904/150‑1 (MQ=255)
                                           aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                            <  1:1848944/150‑1 (MQ=255)
                                           aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                            <  2:334548/150‑1 (MQ=255)
                                            aCAGCGGCAAGATCAAGGGTCGTGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggg                                                                                                                      >  1:1746838/1‑139 (MQ=14)
                                            aCAGCGGCAAGATCAAGGGTCGTGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggg                                                                                                                      <  2:1746838/139‑1 (MQ=14)
                                            aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                            >  2:1872606/1‑149 (MQ=255)
                                            aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                            <  1:1872606/149‑1 (MQ=255)
                                            aCAGCGGCAAGATCAAGGGTCGAGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTTAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                           <  1:1651321/150‑1 (MQ=255)
                                              aGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaa                                                                                                         >  1:873670/1‑150 (MQ=255)
                                                gggCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                       <  2:1755765/149‑1 (MQ=255)
                                                cGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATTCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                       <  1:321278/150‑1 (MQ=255)
                                                  gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAAc                                                                                                                                    >  1:896464/1‑119 (MQ=14)
                                                  gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCt                                                                                                     >  1:603392/1‑150 (MQ=255)
                                                    aaGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGa                                                                                                   <  1:1066590/150‑1 (MQ=255)
                                                      gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAATGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAg                                                                                                 <  1:502558/150‑1 (MQ=255)
                                                      gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAACGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAg                                                                                                 <  2:944511/150‑1 (MQ=255)
                                                        tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTACCAAAGGGGTCAGTAAGTCTAATCTGATGGt                                                                                               >  2:902131/1‑150 (MQ=38)
                                                         cAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                       <  1:1294955/141‑1 (MQ=255)
                                                         cAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                       >  2:1294955/1‑141 (MQ=255)
                                                          aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          <  2:280726/105‑1 (MQ=255)
                                                          aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                                                          >  1:280726/1‑105 (MQ=255)
                                                          aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTc                                                                                             >  2:113195/1‑150 (MQ=255)
                                                                  cgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAATGTTCATACCGTg                                                                                     <  1:1579176/150‑1 (MQ=255)
                                                                      cTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACt                                                                                 >  2:1102147/1‑150 (MQ=255)
                                                                             tCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                  >  2:1149568/1‑110 (MQ=255)
                                                                             tCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                  <  1:1149568/110‑1 (MQ=255)
                                                                                gCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGt                                                                       >  1:276170/1‑150 (MQ=255)
                                                                                   ttATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGat                                                                    >  2:1063889/1‑150 (MQ=255)
                                                                                       cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATTCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                <  1:902131/150‑1 (MQ=255)
                                                                                       cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                <  1:113195/150‑1 (MQ=255)
                                                                                       cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                <  1:1604503/150‑1 (MQ=255)
                                                                                        tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                               >  2:1468959/1‑150 (MQ=255)
                                                                                        tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                               >  1:618875/1‑150 (MQ=255)
                                                                                         aaaGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCt                                                              >  2:847168/1‑150 (MQ=255)
                                                                                          aaGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCtt                                                             >  2:1624292/1‑150 (MQ=255)
                                                                                           aGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                            <  1:1835771/150‑1 (MQ=255)
                                                                                            gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTgg                                                           >  1:555802/1‑150 (MQ=255)
                                                                                             ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTcgc                                                                                                                                            >  1:480479/1‑68 (MQ=255)
                                                                                             ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTcgc                                                                                                                                            <  2:480479/68‑1 (MQ=255)
                                                                                             ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAATGTTCATACCGTGCACTCCACCGACGTGCTCATCCTTggc                                                          >  1:745381/1‑150 (MQ=255)
                                                                                             ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggc                                                          >  1:637201/1‑150 (MQ=255)
                                                                                             ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACAAAATCATCCTTggc                                                          >  1:298678/1‑150 (MQ=255)
                                                                                               aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCGCCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggaga                                                        >  1:1868082/1‑147 (MQ=255)
                                                                                                 gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                                                         >  1:123515/1‑131 (MQ=255)
                                                                                                 gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                                                         <  2:123515/131‑1 (MQ=255)
                                                                                                   aaaGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggc                                                    >  2:1215974/1‑150 (MQ=255)
                                                                                                       cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGgcgccggcgccg                                                >  1:1848435/1‑150 (MQ=255)
                                                                                                           cTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCgg                                            <  1:319430/150‑1 (MQ=255)
                                                                                                            tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCAc                                                                             <  2:141889/116‑1 (MQ=255)
                                                                                                            tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCAc                                                                             >  1:141889/1‑116 (MQ=255)
                                                                                                              tAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCt                                         <  1:557180/150‑1 (MQ=255)
                                                                                                                 gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                                      <  2:618875/150‑1 (MQ=255)
                                                                                                                 gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                                      <  2:78337/150‑1 (MQ=255)
                                                                                                                   gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                    >  2:1454957/1‑150 (MQ=255)
                                                                                                                   gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                    <  2:385404/150‑1 (MQ=255)
                                                                                                                   gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                    <  1:55852/150‑1 (MQ=255)
                                                                                                                       gTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgcgc                                >  2:756625/1‑150 (MQ=255)
                                                                                                                       gTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgcgc                                >  2:114333/1‑150 (MQ=255)
                                                                                                                        tttACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGcc                               <  1:1624292/150‑1 (MQ=255)
                                                                                                                           aCCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAg                            <  2:1184825/150‑1 (MQ=255)
                                                                                                                             ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                                   <  2:854790/61‑1 (MQ=255)
                                                                                                                             ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                                   >  1:854790/1‑61 (MQ=255)
                                                                                                                             ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt                          >  2:522106/1‑150 (MQ=255)
                                                                                                                             ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                                    <  2:1823008/60‑3 (MQ=18)
                                                                                                                             ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                                    >  1:1823008/1‑58 (MQ=18)
                                                                                                                              cgcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTc                         <  1:1468959/150‑1 (MQ=255)
                                                                                                                               gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCa                        <  2:1848435/150‑1 (MQ=255)
                                                                                                                               gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTCCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCAGGCCTGATGTGGGCCTAGCTCa                        >  2:1079027/1‑150 (MQ=255)
                                                                                                                                       ggCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGt                >  1:25031/1‑150 (MQ=255)
                                                                                                                                        gCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTCCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTa               <  1:1079027/150‑1 (MQ=255)
                                                                                                                                           gTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCg            <  1:522106/150‑1 (MQ=255)
                                                                                                                                             gAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGgc          <  1:1643754/150‑1 (MQ=255)
                                                                                                                                              aaTGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGgcc         >  1:1814329/1‑150 (MQ=255)
                                                                                                                                                    ccATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCggg   >  2:1586692/1‑150 (MQ=255)
                                                                                                                                                     cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGt  <  1:1586692/150‑1 (MQ=255)
                                                                                                                                                     cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCACTGCCCGTAGCGTCCGCCgggg  >  2:1385264/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCCGTGGAGCGGGCGCTGGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGT  >  NC_002947/5643176‑5643474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑