Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,643,341 G→A 6.5% intergenic (+75/‑48) dbpA → / → yhiN 23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,643,3410GA 6.5% 273.3 / 5.7 92intergenic (+75/‑48)dbpA/yhiN23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (44/41);  new base A (3/3);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

GGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGTGCTGC  >  NC_002947/5643194‑5643479
                                                                                                                                                   |                                                                                                                                          
ggCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGaa                                                                                                                                          <  1:1263348/150‑1 (MQ=255)
ggCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTaaa                                                                                                                                          <  2:466501/150‑1 (MQ=25)
   cAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAccc                                                                                                                                       >  1:868399/1‑150 (MQ=255)
    aaGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCg                                                                                                                                      >  1:16768/1‑150 (MQ=255)
     aGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                                                                     >  2:1848944/1‑150 (MQ=255)
      gCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCGGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCt                                                                                                                                    >  2:1835771/1‑150 (MQ=255)
          gCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCca                                                                                                                                >  1:878504/1‑150 (MQ=255)
             aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAg                                                                                                                             >  1:334548/1‑150 (MQ=255)
               gCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                                                                           <  2:16768/150‑1 (MQ=255)
                 aGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAATCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                                         <  1:396443/150‑3 (MQ=17)
                  gCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                                        <  2:878504/150‑1 (MQ=255)
                    ggCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAg                                                                                                                      <  1:1581580/150‑1 (MQ=255)
                      cTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCa                                                                                                                    >  1:853947/1‑150 (MQ=255)
                        gAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCAGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                                                                  <  1:613667/150‑1 (MQ=255)
                         aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGTGTCAGCAttt                                                                                                                 <  1:469904/150‑1 (MQ=255)
                         aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                                 <  1:1848944/150‑1 (MQ=255)
                         aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                                 <  2:334548/150‑1 (MQ=255)
                          aCAGCGGCAAGATCAAGGGTCGTGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggg                                                                                                                           >  1:1746838/1‑139 (MQ=14)
                          aCAGCGGCAAGATCAAGGGTCGTGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAggg                                                                                                                           <  2:1746838/139‑1 (MQ=14)
                          aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                                 >  2:1872606/1‑149 (MQ=255)
                          aCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                                                                 <  1:1872606/149‑1 (MQ=255)
                          aCAGCGGCAAGATCAAGGGTCGAGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTTAACCCGCTCCCACAGGGTTCAGCATTTc                                                                                                                <  1:1651321/150‑1 (MQ=255)
                            aGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCaa                                                                                                              >  1:873670/1‑150 (MQ=255)
                              gggCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                            <  2:1755765/149‑1 (MQ=255)
                              cGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATTCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                            <  1:321278/150‑1 (MQ=255)
                                gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAAc                                                                                                                                         >  1:896464/1‑119 (MQ=14)
                                gCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCt                                                                                                          >  1:603392/1‑150 (MQ=255)
                                  aaGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGa                                                                                                        <  1:1066590/150‑1 (MQ=255)
                                    gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAATGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAg                                                                                                      <  1:502558/150‑1 (MQ=255)
                                    gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAACGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAg                                                                                                      <  2:944511/150‑1 (MQ=255)
                                      tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTACCAAAGGGGTCAGTAAGTCTAATCTGATGGt                                                                                                    >  2:902131/1‑150 (MQ=38)
                                       cAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                            >  2:1294955/1‑141 (MQ=255)
                                       cAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAt                                                                                                            <  1:1294955/141‑1 (MQ=255)
                                        aaGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTc                                                                                                  >  2:113195/1‑150 (MQ=255)
                                                cgcCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAATGTTCATACCGTg                                                                                          <  1:1579176/150‑1 (MQ=255)
                                                    cTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACt                                                                                      >  2:1102147/1‑150 (MQ=255)
                                                           tCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                       >  2:1149568/1‑110 (MQ=255)
                                                           tCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCa                                                                                                                       <  1:1149568/110‑1 (MQ=255)
                                                              gCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGt                                                                            >  1:276170/1‑150 (MQ=255)
                                                              gCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGt                                                                            >  1:1868397/1‑150 (MQ=255)
                                                                 ttATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGat                                                                         >  2:1063889/1‑150 (MQ=255)
                                                                     cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATTCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                     <  1:902131/150‑1 (MQ=255)
                                                                     cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                     <  1:1604503/150‑1 (MQ=255)
                                                                     cTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGatcatc                                                                     <  1:113195/150‑1 (MQ=255)
                                                                      tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                                    >  1:618875/1‑150 (MQ=255)
                                                                      tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                                    >  1:827929/1‑150 (MQ=255)
                                                                      tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATcc                                                                    >  2:1468959/1‑150 (MQ=255)
                                                                       aaaGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCt                                                                   >  2:847168/1‑150 (MQ=255)
                                                                        aaGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCtt                                                                  >  2:1624292/1‑150 (MQ=255)
                                                                         aGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                                                                 <  1:1835771/150‑1 (MQ=255)
                                                                          gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTgg                                                                >  1:555802/1‑150 (MQ=255)
                                                                           ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAATGTTCATACCGTGCACTCCACCGACGTGCTCATCCTTggc                                                               >  1:745381/1‑150 (MQ=255)
                                                                           ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggc                                                               >  1:637201/1‑150 (MQ=255)
                                                                           ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACAAAATCATCCTTggc                                                               >  1:298678/1‑150 (MQ=255)
                                                                             aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCGCCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggaga                                                             >  1:1868082/1‑147 (MQ=255)
                                                                               gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                                                              >  1:123515/1‑131 (MQ=255)
                                                                               gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                                                              <  2:123515/131‑1 (MQ=255)
                                                                                 aaaGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggc                                                         >  2:1215974/1‑150 (MQ=255)
                                                                                     cccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGgcgccggcgccg                                                     >  1:1848435/1‑150 (MQ=255)
                                                                                       cccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTCATCATCCTTGGCGCCGGCgccgcc                                                   >  2:954428/1‑150 (MQ=255)
                                                                                         cTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCgg                                                 <  1:319430/150‑1 (MQ=255)
                                                                                          tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCAc                                                                                  >  1:141889/1‑116 (MQ=255)
                                                                                          tGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCAc                                                                                  <  2:141889/116‑1 (MQ=255)
                                                                                            tAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCt                                              <  1:557180/150‑1 (MQ=255)
                                                                                               gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                                           <  2:618875/150‑1 (MQ=255)
                                                                                               gAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAt                                           <  2:78337/150‑1 (MQ=255)
                                                                                                 gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                         <  2:385404/150‑1 (MQ=255)
                                                                                                 gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                         >  2:1454957/1‑150 (MQ=255)
                                                                                                 gCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGAtgt                                         <  1:55852/150‑1 (MQ=255)
                                                                                                     gTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgcgc                                     >  2:114333/1‑150 (MQ=255)
                                                                                                     gTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTgcgc                                     >  2:756625/1‑150 (MQ=255)
                                                                                                      tttACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGcc                                    <  1:1624292/150‑1 (MQ=255)
                                                                                                         aCCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAg                                 <  2:1184825/150‑1 (MQ=255)
                                                                                                           ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                                        <  2:854790/61‑1 (MQ=255)
                                                                                                           ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTc                                                                                                                        >  1:854790/1‑61 (MQ=255)
                                                                                                           ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCt                               >  2:522106/1‑150 (MQ=255)
                                                                                                           ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                                         <  2:1823008/60‑3 (MQ=18)
                                                                                                           ccGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACAggggg                                                                                                                         >  1:1823008/1‑58 (MQ=18)
                                                                                                            cgcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTc                              <  1:1468959/150‑1 (MQ=255)
                                                                                                             gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCa                             <  2:1848435/150‑1 (MQ=255)
                                                                                                             gcgAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTCCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCAGGCCTGATGTGGGCCTAGCTCa                             >  2:1079027/1‑150 (MQ=255)
                                                                                                                     ggCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGt                     >  1:25031/1‑150 (MQ=255)
                                                                                                                      gCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTCCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTa                    <  1:1079027/150‑1 (MQ=255)
                                                                                                                         gTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCg                 <  1:522106/150‑1 (MQ=255)
                                                                                                                           gAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGgc               <  1:1643754/150‑1 (MQ=255)
                                                                                                                            aaTGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGgcc              >  1:1814329/1‑150 (MQ=255)
                                                                                                                                  ccATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCggg        >  2:1586692/1‑150 (MQ=255)
                                                                                                                                   cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGt       <  1:1586692/150‑1 (MQ=255)
                                                                                                                                   cATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCACTGCCCGTAGCGTCCGCCgggg       >  2:1385264/1‑149 (MQ=255)
                                                                                                                                    aTCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGtg      <  1:847168/150‑1 (MQ=255)
                                                                                                                                      cGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGtgct    <  2:868399/150‑1 (MQ=255)
                                                                                                                                                ggTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGtgctgc  <  2:904753/142‑1 (MQ=255)
                                                                                                                                                ggTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGtgctgc  >  1:904753/1‑142 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GGCCAAGCAGGCCATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCTGATGTGCGCCCAGCTCAGTGCCCGTAGCGGCCGCCGGGTGCTGC  >  NC_002947/5643194‑5643479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: