Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 354,881 G→C 8.2% V98V (GTC→GTG)  cbcW ← choline/betaine/carnitine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947354,8810GC 8.2% 213.8 / 6.3 70V98V (GTC→GTG) cbcWcholine/betaine/carnitine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base C (2/4);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTGAA  >  NC_002947/354733‑355030
                                                                                                                                                    |                                                                                                                                                     
gggtcagggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGa                                                                                                                                                      <  1:916929/150‑1 (MQ=255)
   tcagggtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCt                                                                                                                                                   >  1:808649/1‑150 (MQ=255)
        gtAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGcc                                                                                                                                              >  1:652713/1‑150 (MQ=255)
         tAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCa                                                                                                                                             <  2:731990/150‑1 (MQ=255)
           gggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAgg                                                                                                                                           <  1:1191230/150‑1 (MQ=255)
            ggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGCAGGTCACCTTCGCCAcgg                                                                                                                                          <  1:1273335/150‑1 (MQ=255)
             gATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                                         >  2:1221251/1‑150 (MQ=255)
             gATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                                         >  2:1630911/1‑150 (MQ=255)
                   gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCAt                                                                                                                                   >  2:1059188/1‑150 (MQ=255)
                    tggACGAATGTGGGCATCGTCTGCATCATGTCGAGCGCCGGGCGCATGGCTGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                                                  <  2:598821/148‑1 (MQ=255)
                         gAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                             >  1:852443/1‑150 (MQ=255)
                         gAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                             <  1:1394760/150‑1 (MQ=255)
                             gTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                         <  2:343695/150‑1 (MQ=255)
                                ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAg                                                                                                                      >  1:252018/1‑150 (MQ=255)
                                 gCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGt                                                                                                                     >  1:1916530/1‑150 (MQ=255)
                                  cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                             <  1:1829940/141‑1 (MQ=255)
                                  cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                             >  2:1829940/1‑141 (MQ=255)
                                         tGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAgg                                                                                                             >  1:140149/1‑150 (MQ=255)
                                         tGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAgg                                                                                                             <  2:1916530/150‑1 (MQ=255)
                                             tCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGa                                                                                                         <  1:1059188/150‑1 (MQ=255)
                                             tCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGa                                                                                                         <  1:595876/150‑1 (MQ=255)
                                               aGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGGCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAgg                                                                                                       <  2:190991/150‑1 (MQ=255)
                                                ggTCGAGCACCGGGCGCATGGCGGTCTAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCAAGTAACCCAGGTggagga                                                                                                      >  1:646777/1‑150 (MQ=255)
                                                    gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACACAGGTTGAGGATcag                                                                                                  >  1:79853/1‑150 (MQ=255)
                                                    gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                         <  2:111196/111‑1 (MQ=38)
                                                    gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                         <  2:111227/111‑1 (MQ=38)
                                                    gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                         >  1:111196/1‑111 (MQ=38)
                                                    gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                         >  1:111227/1‑111 (MQ=38)
                                                       cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                               <  1:348774/150‑1 (MQ=255)
                                                       cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                               >  2:1336916/1‑150 (MQ=255)
                                                       cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                               >  2:348774/1‑150 (MQ=255)
                                                                 aTGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCTTCCCATTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                                     >  1:101525/1‑150 (MQ=255)
                                                                      ggTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                                <  1:1630911/150‑1 (MQ=255)
                                                                       gTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                                >  1:1355364/1‑133 (MQ=255)
                                                                       gTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                                <  2:1355364/133‑1 (MQ=255)
                                                                         gtaGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                             >  2:1335014/2‑150 (MQ=255)
                                                                          taGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAAt                                                                            <  1:1336916/150‑1 (MQ=255)
                                                                            gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                             <  2:390352/147‑1 (MQ=255)
                                                                            gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                             >  1:390352/1‑147 (MQ=255)
                                                                            gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGGTGGGTATCATTAGAAACGACAGGGCGACAAATGc                                                                          >  2:1070172/1‑150 (MQ=255)
                                                                                           cgcgGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACtt                                                           <  1:1335014/150‑1 (MQ=255)
                                                                                            gcggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                                <  2:1130923/128‑1 (MQ=255)
                                                                                            gcggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                                >  1:1130923/1‑128 (MQ=255)
                                                                                                cgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCt                                                      <  1:219436/150‑1 (MQ=255)
                                                                                                 gAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATCGTTTACTGCAAGTAACCCAGGTCGCGGATCACAAGAAACGACACGGCGACAAATGCGGTCAGTGGCCACTTGGGCTg                                                     >  2:118016/1‑150 (MQ=255)
                                                                                                    gATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                                                  >  2:1844144/1‑150 (MQ=255)
                                                                                                      tGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                                     >  2:951044/1‑113 (MQ=255)
                                                                                                      tGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                                     <  1:951044/113‑1 (MQ=255)
                                                                                                        cccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGaaa                                              <  2:140149/150‑1 (MQ=255)
                                                                                                         ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAAt                                             >  2:393521/1‑150 (MQ=255)
                                                                                                         ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAAt                                             >  1:622306/1‑150 (MQ=255)
                                                                                                           aGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATgc                                           <  1:1844144/150‑1 (MQ=255)
                                                                                                               gCACGCCGATCACCACACATACCACCGTGGCGACGGTCGTCCGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCa                                       <  1:1070172/150‑1 (MQ=255)
                                                                                                               gCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgcgc                                                       >  1:284873/1‑134 (MQ=255)
                                                                                                               gCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgcgc                                                       <  2:284873/134‑1 (MQ=255)
                                                                                                                cACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAg                                      <  2:808649/150‑1 (MQ=255)
                                                                                                                      gATCACCACACATACAACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGATAGGGCGACAAATGCGGTCAGTGCCCACTTGCCCTGGATGAATTGCGCCAGggcggc                                <  2:79853/150‑1 (MQ=255)
                                                                                                                                  tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                           <  1:1843629/79‑1 (MQ=255)
                                                                                                                                  tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                           >  2:1843629/1‑79 (MQ=255)
                                                                                                                                     caccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGGATGGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAg                 >  1:18777/1‑150 (MQ=255)
                                                                                                                                       ccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaa               <  1:1221251/150‑1 (MQ=255)
                                                                                                                                       ccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaa               <  2:652713/150‑1 (MQ=255)
                                                                                                                                          tGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGcc                                                                <  1:328493/98‑1 (MQ=255)
                                                                                                                                          tGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGcc                                                                >  2:328493/1‑98 (MQ=255)
                                                                                                                                              gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                                           <  1:1908593/83‑1 (MQ=255)
                                                                                                                                              gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                                           >  2:1908593/1‑83 (MQ=255)
                                                                                                                                                aGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGt      >  1:204360/1‑150 (MQ=255)
                                                                                                                                                 ggTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGAt                    >  1:87974/1‑135 (MQ=255)
                                                                                                                                                 ggTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGAt                    <  2:87974/135‑1 (MQ=255)
                                                                                                                                                   tGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACATGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTga   <  2:852443/150‑1 (MQ=255)
                                                                                                                                                    gACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGGCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTgaa  >  1:619505/1‑150 (MQ=255)
                                                                                                                                                    gACCGGCGCGAGGGTCTCAATGGTTTCCTGACAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTgca  <  2:101525/150‑3 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGGTCAGGGTAGGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTGAA  >  NC_002947/354733‑355030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑