Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 354,892 G→C 5.4% L95V (CTG→GTG)  cbcW ← choline/betaine/carnitine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947354,8920GC 5.4% 236.5 / 3.1 74L95V (CTG→GTG) cbcWcholine/betaine/carnitine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (35/35);  new base C (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTGAAGA  >  NC_002947/354744‑355032
                                                                                                                                                    |                                                                                                                                            
gggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAgg                                                                                                                                             <  1:1191230/150‑1 (MQ=255)
 ggATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGCAGGTCACCTTCGCCAcgg                                                                                                                                            <  1:1273335/150‑1 (MQ=255)
  gATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                                           >  2:1221251/1‑150 (MQ=255)
  gATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGt                                                                                                                                           >  2:1630911/1‑150 (MQ=255)
        gTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCAt                                                                                                                                     >  2:1059188/1‑150 (MQ=255)
         tggACGAATGTGGGCATCGTCTGCATCATGTCGAGCGCCGGGCGCATGGCTGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATg                                                                                                                                    <  2:598821/148‑1 (MQ=255)
              gAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                               <  1:1394760/150‑1 (MQ=255)
              gAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                               >  1:852443/1‑150 (MQ=255)
                  gTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGc                                                                                                                           <  2:343695/150‑1 (MQ=255)
                     ggCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAg                                                                                                                        >  1:252018/1‑150 (MQ=255)
                      gCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGt                                                                                                                       >  1:1916530/1‑150 (MQ=255)
                       cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                               >  2:1829940/1‑141 (MQ=255)
                       cACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTc                                                                                                                               <  1:1829940/141‑1 (MQ=255)
                              tGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAgg                                                                                                               >  1:140149/1‑150 (MQ=255)
                              tGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAgg                                                                                                               <  2:1916530/150‑1 (MQ=255)
                                  tCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGa                                                                                                           <  1:595876/150‑1 (MQ=255)
                                  tCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGa                                                                                                           <  1:1059188/150‑1 (MQ=255)
                                    aGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGGCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAgg                                                                                                         <  2:190991/150‑1 (MQ=255)
                                     ggTCGAGCACCGGGCGCATGGCGGTCTAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCAAGTAACCCAGGTggagga                                                                                                        >  1:646777/1‑150 (MQ=255)
                                         gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACACAGGTTGAGGATcag                                                                                                    >  1:79853/1‑150 (MQ=255)
                                         gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                           >  1:111227/1‑111 (MQ=38)
                                         gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                           >  1:111196/1‑111 (MQ=38)
                                         gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                           <  2:111196/111‑1 (MQ=38)
                                         gAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCCTGGCGCAGGTCACCTTCGCCACGGt                                                                                                                                           <  2:111227/111‑1 (MQ=38)
                                            cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                                 >  2:348774/1‑150 (MQ=255)
                                            cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                                 >  2:1336916/1‑150 (MQ=255)
                                            cACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagcag                                                                                                 <  1:348774/150‑1 (MQ=255)
                                                      aTGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCTTCCCATTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                                       >  1:101525/1‑150 (MQ=255)
                                                           ggTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                                  <  1:1630911/150‑1 (MQ=255)
                                                            gTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                                  <  2:1355364/133‑1 (MQ=255)
                                                            gTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                                  >  1:1355364/1‑133 (MQ=255)
                                                              gtaGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                               >  2:1335014/2‑150 (MQ=255)
                                                               taGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAAt                                                                              <  1:1336916/150‑1 (MQ=255)
                                                                 gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                               <  2:390352/147‑1 (MQ=255)
                                                                 gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACaaa                                                                               >  1:390352/1‑147 (MQ=255)
                                                                 gAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGGTGGGTATCATTAGAAACGACAGGGCGACAAATGc                                                                            >  2:1070172/1‑150 (MQ=255)
                                                                                cgcgGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACtt                                                             <  1:1335014/150‑1 (MQ=255)
                                                                                 gcggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                                  <  2:1130923/128‑1 (MQ=255)
                                                                                 gcggcgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGAc                                                                                  >  1:1130923/1‑128 (MQ=255)
                                                                                     cgAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCt                                                        <  1:219436/150‑1 (MQ=255)
                                                                                      gAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATCGTTTACTGCAAGTAACCCAGGTCGCGGATCACAAGAAACGACACGGCGACAAATGCGGTCAGTGGCCACTTGGGCTg                                                       >  2:118016/1‑150 (MQ=255)
                                                                                         gATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGAt                                                    >  2:1844144/1‑150 (MQ=255)
                                                                                           tGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                                       >  2:951044/1‑113 (MQ=255)
                                                                                           tGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAgg                                                                                       <  1:951044/113‑1 (MQ=255)
                                                                                             cccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGaaa                                                <  2:140149/150‑1 (MQ=255)
                                                                                              ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAAt                                               >  2:393521/1‑150 (MQ=255)
                                                                                              ccAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAAt                                               >  1:622306/1‑150 (MQ=255)
                                                                                                aGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATgc                                             <  1:1844144/150‑1 (MQ=255)
                                                                                                    gCACGCCGATCACCACACATACCACCGTGGCGACGGTCGTCCGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCa                                         <  1:1070172/150‑1 (MQ=255)
                                                                                                    gCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgcgc                                                         >  1:284873/1‑134 (MQ=255)
                                                                                                    gCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTgcgc                                                         <  2:284873/134‑1 (MQ=255)
                                                                                                     cACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAg                                        <  2:808649/150‑1 (MQ=255)
                                                                                                           gATCACCACACATACAACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGATAGGGCGACAAATGCGGTCAGTGCCCACTTGCCCTGGATGAATTGCGCCAGggcggc                                  <  2:79853/150‑1 (MQ=255)
                                                                                                                       tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                             >  2:1843629/1‑79 (MQ=255)
                                                                                                                       tACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAAc                                                                                             <  1:1843629/79‑1 (MQ=255)
                                                                                                                          caccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGGATGGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAg                   >  1:18777/1‑150 (MQ=255)
                                                                                                                            ccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaa                 <  1:1221251/150‑1 (MQ=255)
                                                                                                                            ccGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaa                 <  2:652713/150‑1 (MQ=255)
                                                                                                                               tGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGcc                                                                  >  2:328493/1‑98 (MQ=255)
                                                                                                                               tGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGcc                                                                  <  1:328493/98‑1 (MQ=255)
                                                                                                                                   gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                                             <  1:1908593/83‑1 (MQ=255)
                                                                                                                                   gAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATg                                                                             >  2:1908593/1‑83 (MQ=255)
                                                                                                                                     aGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGt        >  1:204360/1‑150 (MQ=255)
                                                                                                                                      ggTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGAt                      <  2:87974/135‑1 (MQ=255)
                                                                                                                                      ggTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGAt                      >  1:87974/1‑135 (MQ=255)
                                                                                                                                        tGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACATGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTga     <  2:852443/150‑1 (MQ=255)
                                                                                                                                         gACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGGCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTgaa    >  1:619505/1‑150 (MQ=255)
                                                                                                                                         gACCGGCGCGAGGGTCTCAATGGTTTCCTGACAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTgca    <  2:101525/150‑3 (MQ=255)
                                                                                                                                           ccTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                                  >  1:1240004/1‑54 (MQ=255)
                                                                                                                                           ccTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATcagca                                                                                                  <  2:1240004/54‑1 (MQ=255)
                                                                                                                                           ccTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATGAGAACGAACGGGTTgaaga  <  2:622306/150‑1 (MQ=255)
                                                                                                                                           ccTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTgaaga  >  1:1678387/1‑150 (MQ=255)
                                                                                                                                            cTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCa                                         <  1:1755917/110‑1 (MQ=255)
                                                                                                                                            cTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCa                                         >  2:1755917/1‑110 (MQ=255)
                                                                                                                                                 ccAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaacgaa             <  1:1751435/133‑1 (MQ=255)
                                                                                                                                                 ccAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAgaacgaa             >  2:1751435/1‑133 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GGGATCAGGTAGACGAAGGTGGGCACCGTCTGCATCAGGTCGAGCACCGGGCGCATGGCGGTATAGAACATCGGCTTGTGCGCGGCGAGGATGCCCAGTGGCACGCCGATCACCACACATACCACCGTGGCGAAGGTGACCTGCGCCAGGGTCTCCATGGTTTCCTGCCAGTAACCCAGGTTGAGGATCAGCAGGAACGACAGGGCGACAAATGCGGTCAGTGCCCACTTGCGCTGGATGAAATGCGCCAGGGCGGCGAATAGGGCGATAAGAACGAACGGGTTGAAGA  >  NC_002947/354744‑355032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑