Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,752,784	T→C	7.9%	A232A (GCT→GCC)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,752,784	0	T	C	7.9%	172.4 / 4.2	64	A232A (GCT→GCC)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base C (3/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

GCCGTTCGACCATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTT  >  NC_002947/5752637‑5752933                                                                                                                                                     |                                                                                                                                                       gccgTTCGACCATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCt                                                                                                                                                        <  1:1033643/148‑1 (MQ=255) gccgTTCGACCATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCt                                                                                                                                                        >  2:1033643/1‑148 (MQ=255) gccgTTCGACCACGACCAGGAAACCCGCACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGa                                                                                                                                                      >  2:512992/1‑150 (MQ=255)     ttCGACCATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGaa                                                                                                                                                  <  1:1933206/150‑1 (MQ=255)        gACCATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTa                                                                                                                                               >  2:92364/1‑150 (MQ=255)         aCCATGACCACGAAATCCGTACTGCCAAGTCCAACGCCCTGGAAGAAATTGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTAc                                                                                                                                              <  1:1883508/150‑1 (MQ=255)          ccATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACa                                                                                                                                             <  1:245828/150‑1 (MQ=255)           cATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAg                                                                                                                                            <  1:412585/150‑1 (MQ=255)              gACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTTAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGgcg                                                                                                                                         >  1:1529314/1‑150 (MQ=255)              gACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGgcg                                                                                                                                         >  1:896674/1‑150 (MQ=255)               aCCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCCGACGAAGTACAGgcgc                                                                                                                                        >  1:1721794/1‑150 (MQ=255)                 cACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCACGAACGAACTCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCCGACGAAGTACAGGCGCTg                                                                                                                                      >  2:1815195/1‑150 (MQ=255)                 cACGAAATCCGTACTGCCATGTGCAACGACCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCGAGAAATTATTCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTg                                                                                                                                      >  1:1288543/1‑150 (MQ=255)                        tCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTCCGGGCTCGCAAATA‑cg                                                                                                                              >  2:1019097/1‑150 (MQ=255)                         ccGTACTGCCATGTGCAACTCCCTGGAAGTAATGGGCCCGCCCGTTAAAG‑TCACGCGGCACTTCTTTGCGGCTGCCGGCCAGAACGAAAGCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTTACGAAGTACAGGCGCTGAAATA‑cgt                                                                                                                             <  1:227707/150‑1 (MQ=255)                           gTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTa                                                                                                                                               <  2:193718/131‑1 (MQ=255)                           gTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTa                                                                                                                                               >  1:193718/1‑131 (MQ=255)                            tACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑cgtcgt                                                                                                                          >  2:862177/1‑150 (MQ=255)                               tGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGca                                                                                                                       >  2:1453521/1‑150 (MQ=255)                                      tgCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGtt                                                                                                                <  2:1032033/150‑1 (MQ=255)                                        cAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGc                                                                                                              >  1:1829992/1‑150 (MQ=255)                                        cAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCGGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGc                                                                                                              >  2:1556156/1‑150 (MQ=255)                                          aCGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCt                                                                                                                                                        <  2:1820836/107‑1 (MQ=255)                                          aCGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCt                                                                                                                                                        >  1:1820836/1‑107 (MQ=255)                                          aCGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCg                                                                                                            >  2:1792166/1‑150 (MQ=255)                                                  gaagaaATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTAc                                                                                                    >  1:1687882/1‑150 (MQ=255)                                                            gCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACTCTTACGGCCGTACCg                                                                                          >  1:1360543/1‑150 (MQ=255)                                                             ccAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAgg                                                                                                                                           <  2:774214/101‑1 (MQ=255)                                                             ccAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAgg                                                                                                                                           >  1:774214/1‑101 (MQ=255)                                                                     ttGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCa                                                                                 >  2:776638/1‑150 (MQ=255)                                                                      tGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCAt                                                                                >  1:687674/1‑150 (MQ=255)                                                                        aaGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGc                                                                              >  2:870408/1‑150 (MQ=255)                                                                           ttCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGa                                                                                                           <  2:1455577/118‑1 (MQ=255)                                                                           ttCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGa                                                                                                           >  1:1455577/1‑118 (MQ=255)                                                                            tCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAACTA‑CGCCGTGACCAACGTTGCCGACGCTTACGGCCCTACCGCCGCCTTCACGCCGaa                                                                          <  2:1529314/150‑1 (MQ=255)                                                                            tCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGaa                                                                          <  2:896674/150‑1 (MQ=255)                                                                             cac‑caccacGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAg                                                                         <  1:776638/150‑1 (MQ=255)                                                                              ac‑caccacGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTGAACACCCGGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGCTGCCGCCGCTTACGCCCGCACCCCCCCCTTCATGCCGAAGc                                                                        >  2:360582/1‑150 (MQ=255)                                                                                   ccacGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGc                                                                                                        >  2:22957/1‑114 (MQ=255)                                                                                   ccacGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGc                                                                                                        <  1:22957/114‑1 (MQ=255)                                                                                    cacGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTg                                                                   >  1:467681/1‑150 (MQ=255)                                                                                         aGTGGCGACTGCCGGCCAGAACGAAGTCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACgg                                                              <  1:886711/150‑1 (MQ=255)                                                                                         aGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCCGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGTCGTACCGCCACCTTCATGCCGAAGCCACTGTACgg                                                              <  2:1721794/150‑1 (MQ=255)                                                                                               gACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGTCGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACaa                                                        <  1:512992/150‑1 (MQ=255)                                                                                               gACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACaa                                                        >  2:1339279/1‑150 (MQ=255)                                                                                                aCTGCCGGCAAGAACGAAACCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGCCGCTTCCGGCCGTACCACCACCTTCATGCCGTAGCCACTGTACGGCGACAAc                                                       <  2:1288543/150‑1 (MQ=255)                                                                                                  tGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGc                                                                                                        >  1:1296690/1‑99 (MQ=255)                                                                                                  tGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGc                                                                                                        <  2:1296690/99‑1 (MQ=255)                                                                                                         cAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCgg                                                >  1:1733716/1‑148 (MQ=255)                                                                                                         cAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCgg                                                <  2:1733716/148‑1 (MQ=255)                                                                                                           gAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCAt                                            <  1:870408/150‑1 (MQ=255)                                                                                                           gAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGCCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCAt                                            <  1:360582/150‑1 (MQ=255)                                                                                                            aaCGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATg                                           >  2:1715087/1‑150 (MQ=255)                                                                                                            aaCGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCCGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATg                                           >  1:737964/1‑150 (MQ=255)                                                                                                               gAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCAc                                        >  2:1462050/1‑150 (MQ=255)                                                                                                                  aTCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTg                                     <  1:92364/150‑1 (MQ=255)                                                                                                                   tCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGc                                    >  2:619496/1‑150 (MQ=255)                                                                                                                        gTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATC‑CGGCGTGCACAACGTAGCGAACGCTTAAGGCAAGACCGCCACCTTCATGCCGAAGCCACTGTACGTCAACAACGGCTCGGGCATGCCCGTGCACATg                               >  2:219016/1‑150 (MQ=255)                                                                                                                           aaGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCg                            >  2:1495343/1‑150 (MQ=255)                                                                                                                             gTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACAt                                <  2:1795764/144‑1 (MQ=255)                                                                                                                             gTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACAt                                >  1:1795764/1‑144 (MQ=255)                                                                                                                                cAACACCCTGGTGAAGAAGGCCGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTg                       <  1:1815195/150‑1 (MQ=255)                                                                                                                                 aaCACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTgg                      <  2:687674/150‑1 (MQ=255)                                                                                                                                          gTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTCACGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGc             <  1:1019097/150‑1 (MQ=255)                                                                                                                                            gaagaaGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACTCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCaa           <  2:1360543/150‑1 (MQ=255)                                                                                                                                             aagaagGCTGACGAAGTACAGGCGCTGAAATATCCACAAG‑ACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGCCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAg          <  1:1556156/150‑1 (MQ=255)                                                                                                                                               gaagGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGaa        <  1:619496/150‑1 (MQ=255)                                                                                                                                                  ggCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAacac     <  2:467681/150‑1 (MQ=255)                                                                                                                                                     tGACGAAGTACAGGCGCGGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTGCCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCtt  >  1:384880/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                       GCCGTTCGACCATGACCACGAAATCCGTACTGCCATGTGCAACGCCCTGGAAGAAATGGGCCAGACCGTTGAAGTTCAC‑CACCACGAAGTGGCGACTGCCGGCCAGAACGAAATCGGCGTCAAGTTCAACACCCTGGTGAAGAAGGCTGACGAAGTACAGGCGCTGAAATA‑CGTCGTGCACAACGTTGCCGACGCTTACGGCCGTACCGCCACCTTCATGCCGAAGCCACTGTACGGCGACAACGGCTCGGGCATGCACGTGCACATGTCGATCTGGAAAGACGGCAAGAACACCTT  >  NC_002947/5752637‑5752933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑