Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,790,037	T→G	6.8%	S380A (TCC→GCC)	ynfM →	efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,790,037	0	T	G	6.8%	250.7 / 9.6	88	S380A (TCC→GCC)	ynfM	efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (45/37);  new base G (3/3);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TCCACTGGTGATGGTGATCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGT  >  NC_002947/5789889‑5790175                                                                                                                                                     |                                                                                                                                            tCCACTGGTGATGGTGATCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTc                                                                                                                                            >  2:1359968/1‑150 (MQ=255)       ggtgatggtgatCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGt                                                                                                                                      <  1:944503/150‑1 (MQ=255)        gtgatggtgatCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTg                                                                                                                                     <  1:737925/150‑1 (MQ=255)          gatggtgatCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGc                                                                                                                                   <  1:1359968/150‑1 (MQ=255)                  tCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTAGGTGAGCCGGCCNACGCTAATCGCATTACCTGAGTCCATGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGACTCCAGCGTGGCCGGTAcgg                                                                                                                           >  1:529273/1‑150 (MQ=255)                          ttGCTCGTGTTCCCTTTGGTCTTCTTTGGTGCGCACTCGGTGGCCTGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGcgggcggg                                                                                                                   <  2:529273/149‑1 (MQ=255)                          aTGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGcgggcggg                                                                                                                   <  2:1447135/150‑1 (MQ=255)                                gtgtTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTcc                                                                                                                                           >  1:1903658/1‑120 (MQ=255)                                gtgtTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTcc                                                                                                                                           <  2:1903658/120‑1 (MQ=255)                                gtgtTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTc                                                                                                             >  2:1572341/1‑150 (MQ=255)                                   ttCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTgg                                                                                                          >  1:976940/1‑150 (MQ=255)                                   ttCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACCACGCTGGAGCCAGCGtagta                                                                                                                                  <  1:13732/126‑5 (MQ=255)                                   ttCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCGGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACCACGCTGGAGCCAGCGtagta                                                                                                                                  >  2:13732/1‑122 (MQ=255)                                        cTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCa                                                                                                     >  1:1251543/1‑150 (MQ=255)                                        cTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCa                                                                                                     >  2:1402836/1‑150 (MQ=255)                                        cTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCa                                                                                                     >  2:492507/1‑150 (MQ=255)                                             gCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTggg                                                                                                >  2:234389/1‑150 (MQ=255)                                              cttcttTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGGTCCAGCGTGGcc                                                                                                                                  <  1:1068557/115‑1 (MQ=255)                                              cttcttTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGcc                                                                                                                                  >  2:1068557/1‑115 (MQ=255)                                               ttcttTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTa                                                                                                                              >  1:1670404/1‑118 (MQ=255)                                               ttcttTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTa                                                                                                                              <  2:1670404/118‑1 (MQ=255)                                               ttcttTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGc                                                                                              >  2:225867/1‑150 (MQ=255)                                                tcttTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAATGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCt                                                                                             <  2:1807801/150‑1 (MQ=255)                                                  tttGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTgg                                                                                           >  2:784072/1‑150 (MQ=255)                                                      gTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACg                                                                                       >  2:301762/1‑150 (MQ=255)                                                       tGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTg                                                                                                                                     >  2:447540/1‑103 (MQ=255)                                                       tGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTg                                                                                                                                     <  1:447540/103‑1 (MQ=255)                                                              tCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGGGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCggg                                                                              >  1:1558208/1‑150 (MQ=255)                                                              tCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTg                                                                                            <  1:1013766/137‑1 (MQ=255)                                                              tCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTg                                                                                            >  2:1013766/1‑137 (MQ=255)                                                              tCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCggg                                                                              >  1:1748450/1‑150 (MQ=255)                                                                 gtcgCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTg                                                                           <  1:573630/147‑1 (MQ=255)                                                                 gTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTg                                                                           <  2:1251543/150‑1 (MQ=255)                                                                     ccAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCa                                                                       >  2:1289799/1‑150 (MQ=255)                                                                              ggATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCt                                                              >  2:343589/1‑150 (MQ=255)                                                                               gATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCtt                                                             <  1:234389/150‑1 (MQ=255)                                                                                 tCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTgg                                                                   >  1:1132160/1‑142 (MQ=255)                                                                                 tCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTgg                                                                   <  2:1132160/142‑1 (MQ=255)                                                                                 tCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGc                                                           >  1:1940441/1‑150 (MQ=255)                                                                                   ggCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTg                                                         <  1:492507/150‑1 (MQ=255)                                                                                   ggCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTg                                                         >  2:1665506/1‑150 (MQ=255)                                                                                   ggCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTg                                                         >  2:1750364/1‑150 (MQ=255)                                                                                        ccgGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGt                                                    <  2:1504677/150‑1 (MQ=255)                                                                                         cgGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTg                                                   >  1:9741/1‑150 (MQ=255)                                                                                           ggCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGc                                                 <  1:1750364/150‑1 (MQ=255)                                                                                                tGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACg                                                                                       <  2:962643/108‑1 (MQ=255)                                                                                                tGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACg                                                                                       >  1:962643/1‑108 (MQ=255)                                                                                                tGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGa                                            >  2:857389/1‑150 (MQ=255)                                                                                                tGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGa                                            >  1:485300/1‑150 (MQ=255)                                                                                                tGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCCGGGTGTTCTGGCACCAGTGGGGACGAAAAGGTGAT‑GTGCGGGGCGGTGGCAGCTTGCTGGCGGTGGACTTGa                                            >  1:1884845/1‑150 (MQ=255)                                                                                                 gAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCgggg                                                                                                                  >  1:745408/1‑80 (MQ=255)                                                                                                 gAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCgggg                                                                                                                  <  2:745408/80‑1 (MQ=255)                                                                                                  aaGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGAtg                                          <  2:1500302/150‑1 (MQ=255)                                                                                                    ggCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGAtggt                                        <  1:784072/150‑1 (MQ=255)                                                                                                      ccAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGAtggttg                                      <  2:485300/150‑3 (MQ=255)                                                                                                       cAAGGTGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGc                                     >  2:25616/1‑150 (MQ=255)                                                                                                       cAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGc                                                                             <  2:1110178/110‑1 (MQ=255)                                                                                                       cAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGc                                                                             >  1:1110178/1‑110 (MQ=255)                                                                                                            ggCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGc                                >  2:1733970/1‑150 (MQ=255)                                                                                                             gCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCATCCACGCTGTGGCCTTGATGGTGGCGTTGCa                               >  1:1457942/1‑150 (MQ=255)                                                                                                              cAGGCGGCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCAc                              <  2:665757/150‑1 (MQ=255)                                                                                                                 gCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTg                           >  2:1903377/1‑150 (MQ=255)                                                                                                                      gtcgCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGt                                   <  2:980337/137‑1 (MQ=255)                                                                                                                      gtcgCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGt                                   >  1:980337/1‑137 (MQ=255)                                                                                                                       tcgCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAg                     <  1:1139718/150‑1 (MQ=255)                                                                                                                       tcgCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAg                     <  1:1402836/150‑1 (MQ=255)                                                                                                                       tcgCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAg                     >  2:1155855/1‑150 (MQ=255)                                                                                                                        cgCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGt                    <  2:976940/150‑1 (MQ=255)                                                                                                                         gCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGtt                   >  1:1563409/1‑150 (MQ=255)                                                                                                                         gCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGtt                   <  1:1572341/150‑1 (MQ=255)                                                                                                                          cTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTg                                             >  1:1855793/1‑123 (MQ=255)                                                                                                                          cTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTg                                             <  2:1855793/123‑1 (MQ=255)                                                                                                                             tACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCa               <  1:1665506/150‑1 (MQ=255)                                                                                                                             tACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCa               <  2:9741/150‑1 (MQ=255)                                                                                                                              aCCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCAc              <  1:1289799/150‑1 (MQ=255)                                                                                                                                cTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCAccc            <  1:225867/150‑1 (MQ=255)                                                                                                                                cTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGGTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCAccc            >  2:283863/1‑150 (MQ=255)                                                                                                                                 tGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCa           >  1:1916458/1‑150 (MQ=255)                                                                                                                                  gTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCaa          >  1:1879850/1‑150 (MQ=255)                                                                                                                                        cggccacGCTGGAGCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCAc                              <  2:335227/119‑1 (MQ=255)                                                                                                                                        cggccacGCTGGAGCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCAc                              >  1:335227/6‑124 (MQ=255)                                                                                                                                         tactacGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGcc                                                >  1:1204461/1‑105 (MQ=255)                                                                                                                                         tactacGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGcc                                                <  2:1204461/105‑1 (MQ=255)                                                                                                                                          actacGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGgtgt  >  2:1561050/1‑150 (MQ=255)                                                                                                                                           ctacGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTgg                                                                   >  1:132799/1‑84 (MQ=255)                                                                                                                                           ctacGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTgg                                                                   <  2:132799/84‑1 (MQ=255)                                                                                                                                           ccacGCTGGAGCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGgt    >  2:1015381/3‑147 (MQ=255)                                                                                                                                           ccacGCTGGAGCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGgt    <  1:1015381/145‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            TCCACTGGTGATGGTGATCGTGGGCATGCTGGTGTTCACCTTCGGCTTCTTTGGTGCGCACTCGGTGGCCAGCAGCTGGATCGGCCGCCGGGCGCTGAAGGCCAAGGGGCAGGCGTCGTCGCTGTACCTGTTCTGCTACTACGCTGGCTCCAGCGTGGCCGGTACGGCGGGCGGGGTGTTCTGGCACCAGTGGGGCTGGAACGG‑GATCGGGCTGTTCATTGGCAGCTTGCTGGCGGTGGCCTTGATGGTGGCGTTGCACCTGAGCAAGTTGCCACCCAAAACGGTGT  >  NC_002947/5789889‑5790175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑