| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,951,018 | 0 | C | A | 40.0% | 11.0 / 12.1 | 20 | intergenic (‑123/+34) | fliL/fliK | flagellar protein FliL/flagellar hook‑length control protein FliK |
| Reads supporting (aligned to +/- strand): ref base C (5/7); new base A (5/3); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCC > NC_002947/4950888‑4951154 | ttCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGAGCCCGCgaa < 2:86945/140‑1 (MQ=255)ttCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGAGCCCGCgaa > 1:86945/1‑140 (MQ=255) taatCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGtt < 1:777078/150‑1 (MQ=255) cATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgta < 1:406009/148‑1 (MQ=255) cATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgta > 2:406009/1‑148 (MQ=255) gggTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGTATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGCGATCTCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGa < 1:396609/150‑1 (MQ=255) aaTGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGcc > 2:133086/1‑150 (MQ=255) cttggcttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACaa < 1:133086/150‑1 (MQ=255) ttggcttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAac < 2:185135/150‑1 (MQ=255) ttggcttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGAGCCCGCgaagaa > 1:416707/1‑93 (MQ=255) ttggcttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGAGCCCGCgaagaa < 2:416707/93‑1 (MQ=255) ttgAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGACGACAACCACct > 1:840726/1‑150 (MQ=255) atggatTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgt > 1:192672/1‑85 (MQ=255) atggatTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgt < 2:192672/85‑1 (MQ=255) ttCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGc < 2:840726/150‑1 (MQ=255) ggCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgc > 1:905731/1‑150 (MQ=255) ttcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgcc > 1:709043/1‑150 (MQ=255) ttcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCAGGGAAACATTCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgcc > 2:711345/1‑150 (MQ=255) tcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGc < 2:458716/139‑1 (MQ=255) tcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGc > 1:458716/1‑139 (MQ=255) | TTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCC > NC_002947/4950888‑4951154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |