Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,851,994	G→T	E346D (GAG→GAT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,994	0	G	T	100.0%	63.8 / NA	20	E346D (GAG→GAT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/9);  total (11/9)

GCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGC  >  NC_002947/1851849‑1852136                                                                                                                                                  |                                                                                                                                               gcgACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGGTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGAGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGc                                                                                                                                            >  2:567454/1‑150 (MQ=255)            acCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTAcc                                                                                                                                 >  1:340983/1‑150 (MQ=255)                       cATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCa                                                                                                                      >  2:607258/1‑150 (MQ=255)                        aTCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGGTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCTGTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCAc                                                                                                                     >  1:653200/1‑150 (MQ=255)                                    ttGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGaa                                                                                                         <  1:259769/150‑1 (MQ=255)                                          cggcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                   <  2:258786/150‑1 (MQ=255)                                              atatTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTAt                                                                                               <  2:40314/150‑1 (MQ=255)                                                                acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACa                                                                             >  2:707355/1‑150 (MQ=255)                                                                 cgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACaa                                                                            <  1:672257/150‑1 (MQ=255)                                                                                    aaGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGccc                                                         >  1:529378/1‑150 (MQ=255)                                                                                     aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCa                                                        >  1:521132/1‑150 (MQ=255)                                                                                      ggAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAg                                                       >  2:354611/1‑150 (MQ=255)                                                                                            cgtctgccGCTCGTTGTACAGTTCTGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCCCCaa                                                 <  2:448245/144‑1 (MQ=255)                                                                                                 aCCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGATCACCGACGTCAAGCCCAGCTCCAACCACt                                            <  1:225633/150‑1 (MQ=255)                                                                                                   cGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTAc                                          >  1:202036/1‑150 (MQ=255)                                                                                                      tCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaa                                       <  2:340983/150‑1 (MQ=255)                                                                                                                                       aaGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGcc                                                          <  2:218754/98‑1 (MQ=255)                                                                                                                                       aaGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGcc                                                          >  1:218754/1‑98 (MQ=255)                                                                                                                                         ggTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg    >  1:531269/1‑150 (MQ=255)                                                                                                                                           tGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGGTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGc  <  2:366194/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               GCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGC  >  NC_002947/1851849‑1852136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑