Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,978,615	(C)8→9	coding (30/696 nt)	flgH ←	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,978,607	1	.	C	100.0%	102.5 / NA	32	A13G (GCG→GGG)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (15/17);  total (15/17)

GCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTG  >  NC_002947/4978465‑4978735                                                                                                                                                |                                                                                                                                  gCTATACAGGTTCAGCTCGAAACCGGCATGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑‑ccccccc                                                                                                                           >  2:13387/1‑150 (MQ=255)   tGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑‑ccccccccc                                                                                                                         >  2:517205/1‑149 (MQ=255)   tGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑‑ccccccccc                                                                                                                         >  2:1160186/1‑149 (MQ=255)     tACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑‑cccccccccag                                                                                                                       >  1:44627/1‑147 (MQ=255)          gTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGa                                                                                                                   >  2:1208341/1‑150 (MQ=255)             cTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGaaaa                                                                                                                >  2:956079/1‑150 (MQ=255)              tGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAAc                                                                                                               <  1:1160186/150‑1 (MQ=255)                cTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGATGAAACCTGCCCGCTACACCGC‑CCCCCCCCAGAGCGAAAACgg                                                                                                             >  2:464277/1‑150 (MQ=255)                        cGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGa                                                                                                     >  1:903690/1‑150 (MQ=255)                             tGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACCGACGAtt                                                                                                >  2:439588/1‑150 (MQ=255)                              ggTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCGGGGCGAAAACGGCCAACAAACGAATc                                                                                               >  2:82556/1‑150 (MQ=255)                                tAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCAt                                                                                             <  2:930873/150‑1 (MQ=255)                                         ccGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTAc                                                                                    <  1:1208341/150‑1 (MQ=255)                                           gttgttGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTAc                                                                                    >  2:973687/1‑149 (MQ=255)                                           gttgttGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTAc                                                                                    <  1:973687/149‑1 (MQ=255)                                              gttgGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCAc                                                                               <  2:44627/150‑1 (MQ=255)                                                        gCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCt                                                                     >  2:24889/1‑150 (MQ=255)                                                            gCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATTACACTTACCTCACGGGCGCAGCTGAGt                                                                 <  1:956079/150‑1 (MQ=255)                                                            gCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                 <  1:24889/150‑1 (MQ=255)                                                            gCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGt                                                                 <  1:517205/150‑1 (MQ=255)                                                                   ggTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAggg                                                          <  2:903690/150‑1 (MQ=255)                                                                                               gggTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGa                                                                                                  <  1:774166/82‑1 (MQ=255)                                                                                               gggTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGa                                                                                                  >  2:774166/1‑82 (MQ=255)                                                                                                   cGTTGGGTTTGGGCGGCGGTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGAGCATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATc                          <  1:82556/150‑1 (MQ=255)                                                                                                        ggctttgtcGTCGCTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTc                     <  1:254157/150‑1 (MQ=255)                                                                                                                  tCGGTGCGACGCAACCTGCCAGCAACACCGCCCCCCCCCCAGGGCGAAAACGGACAACAGACCATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGACGTGGa            >  2:227003/1‑150 (MQ=255)                                                                                                                     gTGCGACGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATg        >  1:364540/1‑150 (MQ=255)                                                                                                                           cGCAACCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTAATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTg  <  1:12751/150‑1 (MQ=255)                                                                                                                              aaCCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGTACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTaga                                                    <  1:27549/97‑1 (MQ=255)                                                                                                                              aaCCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTaga                                                    >  2:27549/1‑97 (MQ=255)                                                                                                                              aaCCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTACCGAACGACAGCATCTGGTCGGCCGTGGAAAt         <  2:756745/140‑1 (MQ=255)                                                                                                                              aaCCTGCCAGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAAt         >  1:756745/1‑140 (MQ=255)                                                                                                                                      aGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGa                                            <  2:416802/97‑1 (MQ=255)                                                                                                                                      aGCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGa                                            >  1:416802/1‑97 (MQ=255)                                                                                                                                       gCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTgg                       <  2:388225/117‑1 (MQ=255)                                                                                                                                       gCAACACCGC‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTgg                       >  1:388225/1‑117 (MQ=255)                                                                                                                                                |                                                                                                                                  GCTGTACAGGTTCTGCTCGAAACCGGCCTGGTAGATCGAACCGTTGTTGGCCGCTGCCGGCAACGGGGTGCGCGGCAACACCGGCGCATAGTACGGGTCGTTGGGCTTGGGCGTCGGTGCGACGCAACCTGCCAGCAACACCG‑‑CCCCCCCCAGGGCGAAAACGGACAACAGACGATTCATGACACTTACCTCACGGGCGCAGCTGAGTGACAGGGCTTAGAGCTGTTGAGTAACGAACGACAGCATCTGGTCGGCCGTGGAAATGACTTTG  >  NC_002947/4978465‑4978735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑