| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,188,015:1 | +A | 8.8% | intergenic (+76/‑134) | PP_3683 → / → PP_3684 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,188,015 | 1 | . | A | 8.8% | 205.3 / 15.9 | 57 | intergenic (+76/‑134) | PP_3683/PP_3684 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (29/23); new base A (2/3); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
TATCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTGTTTTTC > NC_002947/4187868‑4188162 | tATCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑Gc > 1:356125/1‑150 (MQ=255) aTCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCt > 1:883750/1‑150 (MQ=255) aGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGc > 2:793369/1‑150 (MQ=255) tggttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCtt < 2:1119731/150‑1 (MQ=255) gttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTgg > 1:791708/1‑150 (MQ=255) gttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTgg > 2:568487/1‑150 (MQ=255) ttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGc > 1:1047632/1‑150 (MQ=255) ttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGc > 1:895274/1‑150 (MQ=255) ttgATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGAGGCAGTCTGCGGCGAACCCATCGGCGG‑GCTAAGCGGCTTGGc > 2:140579/1‑150 (MQ=255) ccTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAAc < 2:1047632/150‑1 (MQ=255) tCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCTTg < 1:220934/140‑1 (MQ=255) tCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTTAGC‑AAGCGGCTTg > 2:220934/1‑140 (MQ=255) tGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCt > 1:136431/1‑150 (MQ=255) gCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACgg > 1:765139/1‑138 (MQ=255) gCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACgg < 2:765139/138‑1 (MQ=255) ccGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTcg < 1:793369/150‑1 (MQ=255) cGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTcgc < 2:791708/150‑1 (MQ=255) cGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTcgc < 1:1092871/150‑1 (MQ=255) gATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTcgcg < 1:971852/150‑1 (MQ=255) gATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCg > 2:193094/1‑150 (MQ=255) attaaGACAAGCCTTCCGAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCt < 2:139406/147‑1 (MQ=255) aCAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GACAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTgcgcgc > 1:1175336/1‑150 (MQ=255) aaGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTCGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTc > 2:470297/1‑150 (MQ=255) aaGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCGCATTACGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTc > 2:887736/1‑150 (MQ=255) ccGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCtt < 2:349602/85‑1 (MQ=255) ccGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCtt > 1:349602/1‑85 (MQ=255) gTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCtt < 2:356125/150‑1 (MQ=255) aGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATc > 2:283826/1‑150 (MQ=255) cGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCaaa < 1:568487/150‑1 (MQ=255) cTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGTCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAg < 1:140579/150‑1 (MQ=255) tttCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGc > 1:677948/1‑150 (MQ=255) ttCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCt < 1:283826/150‑1 (MQ=255) aGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTcgc < 2:375841/100‑1 (MQ=255) aGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTcgc > 1:375841/1‑100 (MQ=255) aGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCAtct > 2:101434/1‑150 (MQ=255) tcGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGc > 1:360261/1‑83 (MQ=255) tcGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGc < 2:360261/83‑1 (MQ=255) gCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCCGGAAGTGT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAg > 1:873664/1‑150 (MQ=255) gCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAg < 2:644020/150‑1 (MQ=255) aCCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGccc < 1:648267/67‑1 (MQ=255) aCCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGccc > 2:648267/1‑67 (MQ=255) aCCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTa < 2:136001/140‑1 (MQ=255) aCCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTa > 1:136001/1‑140 (MQ=255) ggCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTc < 2:883750/150‑1 (MQ=255) ggggCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGcc > 1:680050/1‑150 (MQ=255) ggCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAgct < 1:505969/120‑3 (MQ=255) ggCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGAt > 2:505969/1‑120 (MQ=255) gCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTcc > 2:562642/1‑150 (MQ=255) ggCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGAt > 1:364905/1‑150 (MQ=255) cGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGcc < 1:887736/150‑1 (MQ=255) tGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCtt < 1:1274147/150‑1 (MQ=255) ccAAGCCGCTTAGC‑AAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTc < 2:510206/134‑1 (MQ=255) ccAAGCCGCTTAGC‑AAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTc > 1:510206/1‑134 (MQ=255) cAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCt > 1:248006/1‑150 (MQ=255) cAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCt < 1:56686/150‑1 (MQ=255) ccGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTGttt > 1:895956/1‑150 (MQ=255) cGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTc > 2:383260/1‑118 (MQ=255) cGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTc < 1:383260/118‑1 (MQ=255) cTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTGTTTTTc < 1:193094/150‑1 (MQ=255) | TATCTAGCGTTGGTTGATGCCTCATCGGTAGCTGCCGATCACGATGCCAAGGATGAAGACAAGCCGTCCTAGCAGTCGCTTTCTCAGCTCTCGGCGTTACCTCATGGCGCGCCCCTGGCGGGGCCGGCTGGCGTGAACCAAGCCGCTT‑GCTAAGCGGCTTGGCCCTAACGGGCTTTCATCCTCGCGCCTCCGGCCAAGGCCTGCGCGCTCCGCTTGCCAAATCAAAAGCTCATCTCTAGATAGCTCCCAGTACAACGTGTCGCTTCGCCTCCGATCAAGCCTTGCGCTGTTTTTC > NC_002947/4187868‑4188162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |